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Science 3 July 1998: Vol. 281. no. 5373, pp. 78 - 81 DOI: 10.1126/science.281.5373.78
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Reports
An Inverted Hexagonal Phase of Cationic Liposome-DNA Complexes Related to DNA Release and Delivery
Ilya Koltover,
Tim Salditt,
*
Joachim O. Rädler,
Cyrus R. Safinya
A two-dimensional columnar phase in mixtures of DNA
complexed with cationic liposomes has been found in the lipid
composition regime known to be significantly more efficient at
transfecting mammalian cells in culture compared to the lamellar
(L C) structure of cationic liposome-DNA complexes.
The structure, derived from synchrotron x-ray diffraction, consists of
DNA coated by cationic lipid monolayers and arranged on a
two-dimensional hexagonal lattice (HIIC). Two
membrane-altering pathways induce the L C HIIC transition: one where the spontaneous curvature of
the lipid monolayer is driven negative, and another where the membrane
bending rigidity is lowered with a new class of helper-lipids. Optical microscopy revealed that the L C complexes bind
stably to anionic vesicles (models of cellular membranes), whereas the
more transfectant HIIC complexes are unstable and
rapidly fuse and release DNA upon adhering to anionic vesicles.
Materials Department, Physics Department, and Biochemistry and
Molecular Biology Program, University of California, Santa Barbara, CA
93106, USA.
*
Present address: Sektion Physik der
Ludwig-Maximilians-Universität München,
Geschwister-Scholl-Platz 1, 80539 München, Germany.
Present address: Physikdepartment, Technische
Universität München, Institut für Biophysik (E22),
James Franck-Strasse, 85747 Garching, Germany.
To whom correspondence should be addressed.
Read the Full Text
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