Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 3 July 1998:
Vol. 281. no. 5373, pp. 64 - 71
DOI: 10.1126/science.281.5373.64
Prev | Table of Contents | Next

Research Articles

Complete Structure of the 11-Subunit Bovine Mitochondrial Cytochrome bc1 Complex

So Iwata, * Joong W. Lee, Kengo Okada, dagger John Kyongwon Lee, Momi Iwata, Bjarne Rasmussen, Thomas A. Link, S. Ramaswamy, Bing K. Jap *

Mitochondrial cytochrome bc1 complex performs two functions: It is a respiratory multienzyme complex and it recognizes a mitochondrial targeting presequence. Refined crystal structures of the 11-subunit bc1 complex from bovine heart reveal full views of this bifunctional enzyme. The "Rieske" iron-sulfur protein subunit shows significant conformational changes in different crystal forms, suggesting a new electron transport mechanism of the enzyme. The mitochondrial targeting presequence of the "Rieske" protein (subunit 9) is lodged between the two "core" subunits at the matrix side of the complex. These "core" subunits are related to the matrix processing peptidase, and the structure unveils how mitochondrial targeting presequences are recognized.

J. W. Lee, J. K. Lee, and B. K. Jap are in the Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA. S. Iwata, K. Okada, and M. Iwata are in the Department of Biochemistry, Uppsala University, BMC, Box , Uppsala S-75123, Sweden. S. Ramaswamy is in the Department of Molecular Biology, Swedish Agricultural Science University, Box , Biomedical Center, Uppsala S-75124, Sweden. T. A. Link is at Uniklinikum Frankfurt, ZBC, Biochemie I, Molecular Bioenergetics, D-60590 Frankfurt/Main, Germany. B. Rasmussen is at the Grenoble Outstation, European Molecular Biology Laboratory, c/o Institut Laue-Langevin, Boîte Postale 156, F-38042 Grenoble Cedex 9, France.
*   To whom correspondence should be addressed. E-mail: BKJap{at}lbl.gov (B.K.J.) or iwata{at}xray.bmc.uu.se (S.I.)

dagger    Present address: Nara Institute of Science and Technology, Division of Structural Biology, Department of Molecular Biology, Graduate School of Biological Sciences, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mitochondrial Dysfunction Increases Allergic Airway Inflammation.
L. Aguilera-Aguirre, A. Bacsi, A. Saavedra-Molina, A. Kurosky, S. Sur, and I. Boldogh (2009)
J. Immunol. 183, 5379-5387
   Abstract »    Full Text »    PDF »
An N-terminal addressing sequence targets NLRX1 to the mitochondrial matrix.
D. Arnoult, F. Soares, I. Tattoli, C. Castanier, D. J. Philpott, and S. E. Girardin (2009)
J. Cell Sci. 122, 3161-3168
   Abstract »    Full Text »    PDF »
Mitochondrial Dysfunction Contributes to Oncogene-Induced Senescence.
O. Moiseeva, V. Bourdeau, A. Roux, X. Deschenes-Simard, and G. Ferbeyre (2009)
Mol. Cell. Biol. 29, 4495-4507
   Abstract »    Full Text »    PDF »
Stigmatellin Induces Reduction of Iron-Sulfur Protein in the Oxidized Cytochrome bc1 Complex.
B. Gurung, L. Yu, and C.-A. Yu (2008)
J. Biol. Chem. 283, 28087-28094
   Abstract »    Full Text »    PDF »
On the Mechanism of Quinol Oxidation at the QP Site in the Cytochrome bc1 Complex: STUDIED USING MUTANTS LACKING CYTOCHROME bL OR bH.
S. Yang, H.-W. Ma, L. Yu, and C.-A. Yu (2008)
J. Biol. Chem. 283, 28767-28776
   Abstract »    Full Text »    PDF »
Functional genetic screening reveals the role of mitochondrial cytochrome b as a mediator of FAS-induced apoptosis.
A. P. Komarov, O. W. Rokhlin, C.-A. Yu, and A. V. Gudkov (2008)
PNAS 105, 14453-14458
   Abstract »    Full Text »    PDF »
Amino Acid Residues Interacting with Both the Bound Quinone and Coenzyme, Pyrroloquinoline Quinone, in Escherichia coli Membrane-bound Glucose Dehydrogenase.
G. Mustafa, Y. Ishikawa, K. Kobayashi, C. T. Migita, M. D. Elias, S. Nakamura, S. Tagawa, and M. Yamada (2008)
J. Biol. Chem. 283, 22215-22221
   Abstract »    Full Text »    PDF »
Structure of glycerol-3-phosphate dehydrogenase, an essential monotopic membrane enzyme involved in respiration and metabolism.
J. I. Yeh, U. Chinte, and S. Du (2008)
PNAS 105, 3280-3285
   Abstract »    Full Text »    PDF »
Inhibitor-complexed Structures of the Cytochrome bc1 from the Photosynthetic Bacterium Rhodobacter sphaeroides.
L. Esser, M. Elberry, F. Zhou, C.-A. Yu, L. Yu, and D. Xia (2008)
J. Biol. Chem. 283, 2846-2857
   Abstract »    Full Text »    PDF »
Oxygen sensing in hypoxic pulmonary vasoconstriction: using new tools to answer an age-old question.
G. B. Waypa and P. T. Schumacker (2008)
Exp Physiol 93, 133-138
   Abstract »    Full Text »    PDF »
Functional Characterization of Mia40p, the Central Component of the Disulfide Relay System of the Mitochondrial Intermembrane Space.
B. Grumbt, V. Stroobant, N. Terziyska, L. Israel, and K. Hell (2007)
J. Biol. Chem. 282, 37461-37470
   Abstract »    Full Text »    PDF »
Histidine Cycle Mechanism for the Concerted Proton/Electron Transfer from Ascorbate to the Cytosolic Haem b Centre of Cytochrome b561: A Unique Machinery for the Biological Transmembrane Electron Transfer.
N. Nakanishi, F. Takeuchi, and M. Tsubaki (2007)
J. Biochem. 142, 553-560
   Abstract »    Full Text »    PDF »
Hydrogen Bonds between Nitrogen Donors and the Semiquinone in the Qi-site of the bc1 Complex.
S. A. Dikanov, J. T. Holland, B. Endeward, D. R. J. Kolling, R. I. Samoilova, T. F. Prisner, and A. R. Crofts (2007)
J. Biol. Chem. 282, 25831-25841
   Abstract »    Full Text »    PDF »
Detection of Reactive Oxygen Species-sensitive Thiol Proteins by Redox Difference Gel Electrophoresis: IMPLICATIONS FOR MITOCHONDRIAL REDOX SIGNALING.
T. R. Hurd, T. A. Prime, M. E. Harbour, K. S. Lilley, and M. P. Murphy (2007)
J. Biol. Chem. 282, 22040-22051
   Abstract »    Full Text »    PDF »
Impaired complex III assembly associated with BCS1L gene mutations in isolated mitochondrial encephalopathy.
E. Fernandez-Vizarra, M. Bugiani, P. Goffrini, F. Carrara, L. Farina, E. Procopio, A. Donati, G. Uziel, I. Ferrero, and M. Zeviani (2007)
Hum. Mol. Genet. 16, 1241-1252
   Abstract »    Full Text »    PDF »
A semiquinone intermediate generated at the Qo site of the cytochrome bc1 complex: Importance for the Q-cycle and superoxide production.
J. L. Cape, M. K. Bowman, and D. M. Kramer (2007)
PNAS 104, 7887-7892
   Abstract »    Full Text »    PDF »
Simultaneous reduction of iron-sulfur protein and cytochrome bL during ubiquinol oxidation in cytochrome bc1 complex.
J. Zhu, T. Egawa, S.-R. Yeh, L. Yu, and C.-A. Yu (2007)
PNAS 104, 4864-4869
   Abstract »    Full Text »    PDF »
Inhibition of Reversed Electron Transfer and Proton Transport in the Beef Heart Cytochrome bc1 Complex by Chemical Modification.
T. Miki, M. Umeda, and Y. Harada (2007)
J. Biochem. 141, 377-387
   Abstract »    Full Text »    PDF »
Catch me if you can! Oxidative protein trapping in the intermembrane space of mitochondria.
J. M. Herrmann and R. Kohl (2007)
J. Cell Biol. 176, 559-563
   Abstract »    Full Text »    PDF »
Similar Transition States Mediate the Q-cycle and Superoxide Production by the Cytochrome bc1 Complex.
I. Forquer, R. Covian, M. K. Bowman, B. L. Trumpower, and D. M. Kramer (2006)
J. Biol. Chem. 281, 38459-38465
   Abstract »    Full Text »    PDF »
Physiological diversity of mitochondrial oxidative phosphorylation.
G. Benard, B. Faustin, E. Passerieux, A. Galinier, C. Rocher, N. Bellance, J.-P. Delage, L. Casteilla, T. Letellier, and R. Rossignol (2006)
Am J Physiol Cell Physiol 291, C1172-C1182
   Abstract »    Full Text »    PDF »
Modeling mitochondrial function.
R. S. Balaban (2006)
Am J Physiol Cell Physiol 291, C1107-C1113
   Abstract »    Full Text »    PDF »
Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool.
J. Zhang, F. E. Frerman, and J.-J. P. Kim (2006)
PNAS 103, 16212-16217
   Abstract »    Full Text »    PDF »
Definition of the mitochondrial proteome by measurement of molecular masses of membrane proteins.
J. Carroll, I. M. Fearnley, and J. E. Walker (2006)
PNAS 103, 16170-16175
   Abstract »    Full Text »    PDF »
Oxygen sensing by mitochondria at complex III: the paradox of increased reactive oxygen species during hypoxia.
R. D. Guzy and P. T. Schumacker (2006)
Exp Physiol 91, 807-819
   Abstract »    Full Text »    PDF »
Gene expression analysis exposes mitochondrial abnormalities in a mouse model of rett syndrome..
S. Kriaucionis, A. Paterson, J. Curtis, J. Guy, N. MacLeod, and A. Bird (2006)
Mol. Cell. Biol. 26, 5033-5042
   Abstract »    Full Text »    PDF »
Deleterious Epistatic Interactions Between Electron Transport System Protein-Coding Loci in the Copepod Tigriopus californicus.
C. S. Willett (2006)
Genetics 173, 1465-1477
   Abstract »    Full Text »    PDF »
Architecture of Active Mammalian Respiratory Chain Supercomplexes.
E. Schafer, H. Seelert, N. H. Reifschneider, F. Krause, N. A. Dencher, and J. Vonck (2006)
J. Biol. Chem. 281, 15370-15375
   Abstract »    Full Text »    PDF »
Reactive oxygen species production by mitochondria in endothelial cells exposed to reoxygenation after hypoxia and glucose depletion is mediated by ceramide.
S. Therade-Matharan, E. Laemmel, S. Carpentier, Y. Obata, T. Levade, J. Duranteau, and E. Vicaut (2005)
Am J Physiol Regulatory Integrative Comp Physiol 289, R1756-R1762
   Abstract »    Full Text »    PDF »
The Iron-Sulfur Cluster of the Rieske Iron-Sulfur Protein Functions as a Proton-exiting Gate in the Cytochrome bc1 Complex.
B. Gurung, L. Yu, D. Xia, and C.-A. Yu (2005)
J. Biol. Chem. 280, 24895-24902
   Abstract »    Full Text »    PDF »
Structure of the Intermolecular Complex between Plastocyanin and Cytochrome f from Spinach.
F. Musiani, A. Dikiy, A. Y. Semenov, and S. Ciurli (2005)
J. Biol. Chem. 280, 18833-18841
   Abstract »    Full Text »    PDF »
Cytochrome b6 Arginine 214 of Synechococcus sp. PCC 7002, a Key Residue for Quinone-reductase Site Function and Turnover of the Cytochrome bf Complex.
M. E. Nelson, G. Finazzi, Q. J. Wang, K. A. Middleton-Zarka, J. Whitmarsh, and T. Kallas (2005)
J. Biol. Chem. 280, 10395-10402
   Abstract »    Full Text »    PDF »
Evidence for Electron Equilibrium between the Two Hemes bL in the Dimeric Cytochrome bc1 Complex.
X. Gong, L. Yu, D. Xia, and C.-A. Yu (2005)
J. Biol. Chem. 280, 9251-9257
   Abstract »    Full Text »    PDF »
Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III.
N. V. Dudkina, H. Eubel, W. Keegstra, E. J. Boekema, and H.-P. Braun (2005)
PNAS 102, 3225-3229
   Abstract »    Full Text »    PDF »
Physicochemical Evolution and Molecular Adaptation of the Cetacean and Artiodactyl Cytochrome b Proteins.
D. A. McClellan, E. J. Palfreyman, M. J. Smith, J. L. Moss, R. G. Christensen, and J. K. Sailsbery (2005)
Mol. Biol. Evol. 22, 437-455
   Abstract »    Full Text »    PDF »
The Mitochondrial Oxidative Phosphorylation Proteome of Chlamydomonas reinhardtii Deduced from the Genome Sequencing Project.
P. Cardol, D. Gonzalez-Halphen, A. Reyes-Prieto, D. Baurain, R. F. Matagne, and C. Remacle (2005)
Plant Physiology 137, 447-459
   Full Text »    PDF »
Hypoxic pulmonary vasoconstriction: redox events in oxygen sensing.
G. B. Waypa and P. T. Schumacker (2005)
J Appl Physiol 98, 404-414
   Abstract »    Full Text »    PDF »
Mitochondrial cytochrome c1 is a collapsed di-heme cytochrome.
F. Baymann, E. Lebrun, and W. Nitschke (2004)
PNAS 101, 17737-17740
   Abstract »    Full Text »    PDF »
Complex III Releases Superoxide to Both Sides of the Inner Mitochondrial Membrane.
F. L. Muller, Y. Liu, and H. Van Remmen (2004)
J. Biol. Chem. 279, 49064-49073
   Abstract »    Full Text »    PDF »
Reoxygenation after hypoxia and glucose depletion causes reactive oxygen species production by mitochondria in HUVEC.
S. Therade-Matharan, E. Laemmel, J. Duranteau, and E. Vicaut (2004)
Am J Physiol Regulatory Integrative Comp Physiol 287, R1037-R1043
   Abstract »    Full Text »    PDF »
The Chloroplast Rieske Iron-Sulfur Protein: AT THE CROSSROAD OF ELECTRON TRANSPORT AND SIGNAL TRANSDUCTION.
C. de Vitry, Y. Ouyang, G. Finazzi, F.-A. Wollman, and T. Kallas (2004)
J. Biol. Chem. 279, 44621-44627
   Abstract »    Full Text »    PDF »
The Vaccinia Virus G1L Putative Metalloproteinase Is Essential for Viral Replication In Vivo.
M. Hedengren-Olcott, C. M. Byrd, J. Watson, and D. E. Hruby (2004)
J. Virol. 78, 9947-9953
   Abstract »    Full Text »    PDF »
Computational analysis of {alpha}-helical membrane protein structure: implications for the prediction of 3D structural models.
T. A. Eyre, L. Partridge, and J. M. Thornton (2004)
Protein Eng. Des. Sel. 17, 613-624
   Abstract »    Full Text »    PDF »
QO Site Deficiency Can Be Compensated by Extragenic Mutations in the Hinge Region of the Iron-Sulfur Protein in the bc1 Complex of Saccharomyces cerevisiae.
G. Brasseur, D. Lemesle-Meunier, F. Reinaud, and B. Meunier (2004)
J. Biol. Chem. 279, 24203-24211
   Abstract »    Full Text »    PDF »
Proteomic Identification of Annexins, Calcium-Dependent Membrane Binding Proteins That Mediate Osmotic Stress and Abscisic Acid Signal Transduction in Arabidopsis.
S. Lee, E. J. Lee, E. J. Yang, J. E. Lee, A. R. Park, W. H. Song, and O. K. Park (2004)
PLANT CELL 16, 1378-1391
   Abstract »    Full Text »    PDF »
Structural Basis of Redox-coupled Protein Substrate Selection by the Cytochrome c Biosynthesis Protein ResA.
A. Crow, R. M. Acheson, N. E. Le Brun, and A. Oubrie (2004)
J. Biol. Chem. 279, 23654-23660
   Abstract »    Full Text »    PDF »
Engineering a Three-cysteine, One-histidine Ligand Environment into a New Hyperthermophilic Archaeal Rieske-type [2Fe-2S] Ferredoxin from Sulfolobus solfataricus.
A. Kounosu, Z. Li, N. J. Cosper, J. E. Shokes, R. A. Scott, T. Imai, A. Urushiyama, and T. Iwasaki (2004)
J. Biol. Chem. 279, 12519-12528
   Abstract »    Full Text »    PDF »
Transmembrane protein domains rarely use covalent domain recombination as an evolutionary mechanism.
Y. Liu, M. Gerstein, and D. M. Engelman (2004)
PNAS 101, 3495-3497
   Abstract »    Full Text »    PDF »
Inhibition of the Yeast Cytochrome bc1 Complex by Ilicicolin H, a Novel Inhibitor That Acts at the Qn Site of the bc1 Complex.
E. B. Gutierrez-Cirlos, T. Merbitz-Zahradnik, and B. L. Trumpower (2004)
J. Biol. Chem. 279, 8708-8714
   Abstract »    Full Text »    PDF »
Evolution of Interacting Proteins in the Mitochondrial Electron Transport System in a Marine Copepod.
C. S. Willett and R. S. Burton (2004)
Mol. Biol. Evol. 21, 443-453
   Abstract »    Full Text »    PDF »
Assembly of Respiratory Complexes I, III, and IV into NADH Oxidase Supercomplex Stabilizes Complex I in Paracoccus denitrificans.
A. Stroh, O. Anderka, K. Pfeiffer, T. Yagi, M. Finel, B. Ludwig, and H. Schagger (2004)
J. Biol. Chem. 279, 5000-5007
   Abstract »    Full Text »    PDF »
SCOP database in 2004: refinements integrate structure and sequence family data.
A. Andreeva, D. Howorth, S. E. Brenner, T. J. P. Hubbard, C. Chothia, and A. G. Murzin (2004)
Nucleic Acids Res. 32, D226-229
   Abstract »    Full Text »    PDF »
Crystal Structure of the RC-LH1 Core Complex from Rhodopseudomonas palustris.
A. W. Roszak, T. D. Howard, J. Southall, A. T. Gardiner, C. J. Law, N. W. Isaacs, and R. J. Cogdell (2003)
Science 302, 1969-1972
   Abstract »    Full Text »    PDF »
Structure of the Cytochrome b6f Complex of Oxygenic Photosynthesis: Tuning the Cavity.
G. Kurisu, H. Zhang, J. L. Smith, and W. A. Cramer (2003)
Science 302, 1009-1014
   Abstract »    Full Text »    PDF »
Effect of Glutathione Depletion on Sites and Topology of Superoxide and Hydrogen Peroxide Production in Mitochondria.
D. Han, R. Canali, D. Rettori, and N. Kaplowitz (2003)
Mol. Pharmacol. 64, 1136-1144
   Abstract »    Full Text »    PDF »
Exploration of Ligands to the Qi Site Semiquinone in the bc1 Complex Using High-resolution EPR.
D. R. J. Kolling, R. I. Samoilova, J. T. Holland, E. A. Berry, S. A. Dikanov, and A. R. Crofts (2003)
J. Biol. Chem. 278, 39747-39754
   Abstract »    Full Text »    PDF »
Production of Reactive Oxygen Species by Mitochondria: CENTRAL ROLE OF COMPLEX III.
Q. Chen, E. J. Vazquez, S. Moghaddas, C. L. Hoppel, and E. J. Lesnefsky (2003)
J. Biol. Chem. 278, 36027-36031
   Abstract »    Full Text »    PDF »
The Modified Q-cycle Explains the Apparent Mismatch between the Kinetics of Reduction of Cytochromes c1 and bH in the bc1 Complex.
A. R. Crofts, V. P. Shinkarev, D. R. J. Kolling, and S. Hong (2003)
J. Biol. Chem. 278, 36191-36201
   Abstract »    Full Text »    PDF »
Transcript Mapping and Genome Annotation of Ascidian mtDNA Using EST Data.
C. Gissi and G. Pesole (2003)
Genome Res. 13, 2203-2212
   Abstract »    Full Text »    PDF »
Structure of the Yeast Cytochrome bc1 Complex with a Hydroxyquinone Anion Qo Site Inhibitor Bound.
H. Palsdottir, C. G. Lojero, B. L. Trumpower, and C. Hunte (2003)
J. Biol. Chem. 278, 31303-31311
   Abstract »    Full Text »    PDF »
"Ping-Pong" Interactions between Mitochondrial tRNA Import Receptors within a Multiprotein Complex.
S. N. Bhattacharyya, S. Chatterjee, S. Goswami, G. Tripathi, S. N. Dey, and S. Adhya (2003)
Mol. Cell. Biol. 23, 5217-5224
   Abstract »    Full Text »    PDF »
Functional Respiratory Chain Analyses in Murid Xenomitochondrial Cybrids Expose Coevolutionary Constraints of Cytochrome b and Nuclear Subunits of Complex III.
M. McKenzie, M. Chiotis, C. A. Pinkert, and I. A. Trounce (2003)
Mol. Biol. Evol. 20, 1117-1124
   Abstract »    Full Text »    PDF »
Functional Insensitivity of the Cytochrome b6f Complex to Structure Changes in the Hinge Region of the Rieske Iron-Sulfur Protein.
J. Yan and W. A. Cramer (2003)
J. Biol. Chem. 278, 20925-20933
   Abstract »    Full Text »    PDF »
A defined protein-detergent-lipid complex for crystallization of integral membrane proteins: The cytochrome b6f complex of oxygenic photosynthesis.
H. Zhang, G. Kurisu, J. L. Smith, and W. A. Cramer (2003)
PNAS 100, 5160-5163
   Abstract »    Full Text »    PDF »
Effect of Famoxadone on Photoinduced Electron Transfer between the Iron-Sulfur Center and Cytochrome c1 in the Cytochrome bc1 Complex.
K. Xiao, G. Engstrom, S. Rajagukguk, C.-A. Yu, L. Yu, B. Durham, and F. Millett (2003)
J. Biol. Chem. 278, 11419-11426
   Abstract »    Full Text »    PDF »
Structure of the bc1 Complex from Seculamonas ecuadoriensis, a Jakobid Flagellate with an Ancestral Mitochondrial Genome.
S. Marx, M. Baumgartner, S. Kunnan, H.-P. Braun, B. F. Lang, and G. Burger (2003)
Mol. Biol. Evol. 20, 145-153
   Abstract »    Full Text »    PDF »
Failure to Insert the Iron-Sulfur Cluster into the Rieske Iron-Sulfur Protein Impairs Both Center N and Center P of the Cytochrome bc1 Complex.
E. B. Gutierrez-Cirlos, T. Merbitz-Zahradnik, and B. L. Trumpower (2002)
J. Biol. Chem. 277, 50703-50709
   Abstract »    Full Text »    PDF »
A Pathogenic Cytochrome b Mutation Reveals New Interactions between Subunits of the Mitochondrial bc1 Complex.
Y. Saint-Georges, N. Bonnefoy, J. P. di Rago, S. Chiron, and G. Dujardin (2002)
J. Biol. Chem. 277, 49397-49402
   Abstract »    Full Text »    PDF »
Topology of Superoxide Production from Different Sites in the Mitochondrial Electron Transport Chain.
J. St-Pierre, J. A. Buckingham, S. J. Roebuck, and M. D. Brand (2002)
J. Biol. Chem. 277, 44784-44790
   Abstract »    Full Text »    PDF »
Electron Transfer from the Rieske Iron-Sulfur Protein (ISP) to Cytochrome f in Vitro. IS A GUIDED TRAJECTORY OF THE ISP NECESSARY FOR COMPETENT DOCKING?.
G. M. Soriano, L.-W. Guo, C. de Vitry, T. Kallas, and W. A. Cramer (2002)
J. Biol. Chem. 277, 41865-41871
   Abstract »    Full Text »    PDF »
Photoinduced Electron Transfer between the Rieske Iron-Sulfur Protein and Cytochrome c1 in the Rhodobacter sphaeroides Cytochrome bc1 Complex. EFFECTS OF pH, TEMPERATURE, AND DRIVING FORCE.
G. Engstrom, K. Xiao, C.-A. Yu, L. Yu, B. Durham, and F. Millett (2002)
J. Biol. Chem. 277, 31072-31078
   Abstract »    Full Text »    PDF »
Protein Oxidation of Cytochrome c by Reactive Halogen Species Enhances Its Peroxidase Activity.
Y.-R. Chen, L. J. Deterding, B. E. Sturgeon, K. B. Tomer, and R. P. Mason (2002)
J. Biol. Chem. 277, 29781-29791
   Abstract »    Full Text »    PDF »
Natural Resistance to Inhibitors of the Ubiquinol Cytochrome c Oxidoreductase of Rubrivivax gelatinosus: Sequence and Functional Analysis of the Cytochrome bc1 Complex.
S. Ouchane, I. Agalidis, and C. Astier (2002)
J. Bacteriol. 184, 3815-3822
   Abstract »    Full Text »    PDF »
Crystallographic Studies of the Escherichia coli Quinol-Fumarate Reductase with Inhibitors Bound to the Quinol-binding Site.
T. M. Iverson, C. Luna-Chavez, L. R. Croal, G. Cecchini, and D. C. Rees (2002)
J. Biol. Chem. 277, 16124-16130
   Abstract »    Full Text »    PDF »
Chimeric Fusions of Subunit IV and PetL in the b6f Complex of Chlamydomonas reinhardtii. STRUCTURAL IMPLICATIONS AND CONSEQUENCES ON STATE TRANSITIONS.
F. Zito, J. Vinh, J.-L. Popot, and G. Finazzi (2002)
J. Biol. Chem. 277, 12446-12455
   Abstract »    Full Text »    PDF »
Molecular Basis of Proton Motive Force Generation: Structure of Formate Dehydrogenase-N.
M. Jormakka, S. Tornroth, B. Byrne, and S. Iwata (2002)
Science 295, 1863-1868
   Abstract »    Full Text »    PDF »
From the Cover: Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c.
C. Lange and C. Hunte (2002)
PNAS 99, 2800-2805
   Abstract »    Full Text »    PDF »
The Interaction of the Rieske Iron-Sulfur Protein with Occupants of the Qo-site of the bc1 Complex, Probed by Electron Spin Echo Envelope Modulation.
R. I. Samoilova, D. Kolling, T. Uzawa, T. Iwasaki, A. R. Crofts, and S. A. Dikanov (2002)
J. Biol. Chem. 277, 4605-4608
   Abstract »    Full Text »    PDF »
C-terminal Periplasmic Domain of Escherichia coli Quinoprotein Glucose Dehydrogenase Transfers Electrons to Ubiquinone.
MD. Elias, M. Tanaka, M. Sakai, H. Toyama, K. Matsushita, O. Adachi, and M. Yamada (2001)
J. Biol. Chem. 276, 48356-48361
   Abstract »    Full Text »    PDF »
Evidence for the Intertwined Dimer of the Cytochrome bc1 Complex in Solution.
K. Xiao, A. Chandrasekaran, L. Yu, and C.-A. Yu (2001)
J. Biol. Chem. 276, 46125-46131
   Abstract »    Full Text »    PDF »
The nitrite reductase from Pseudomonas aeruginosa: Essential role of two active-site histidines in the catalytic and structural properties.
F. Cutruzzola, K. Brown, E. K. Wilson, A. Bellelli, M. Arese, M. Tegoni, C. Cambillau, and M. Brunori (2001)
PNAS 98, 2232-2237
   Abstract »    Full Text »    PDF »
Mutations in Plasmodium falciparum Cytochrome b That Are Associated with Atovaquone Resistance Are Located at a Putative Drug-Binding Site.
M. Korsinczky, N. Chen, B. Kotecka, A. Saul, K. Rieckmann, and Q. Cheng (2000)
Antimicrob. Agents Chemother. 44, 2100-2108
   Abstract »    Full Text »
Dynamic Interaction of Plastocyanin with the Cytochrome bf Complex.
J. Illerhaus, L. Altschmied, J. Reichert, E. Zak, R. G. Herrmann, and W. Haehnel (2000)
J. Biol. Chem. 275, 17590-17595
   Abstract »    Full Text »    PDF »
Mutational Analysis of the sbo-alb Locus of Bacillus subtilis: Identification of Genes Required for Subtilosin Production and Immunity.
G. Zheng, R. Hehn, and P. Zuber (2000)
J. Bacteriol. 182, 3266-3273
   Abstract »    Full Text »
Internal packing of helical membrane proteins.
M. Eilers, S. C. Shekar, T. Shieh, S. O. Smith, and P. J. Fleming (2000)
PNAS 97, 5796-5801
   Abstract »    Full Text »    PDF »
Conformational Changes in the Cytochrome b6f Complex Induced by Inhibitor Binding.
C. Breyton (2000)
J. Biol. Chem. 275, 13195-13201
   Abstract »    Full Text »    PDF »
Uncovering the [2Fe2S] domain movement in cytochrome bc1 and its implications for energy conversion.
E. Darrouzet, M. Valkova-Valchanova, C. C. Moser, P. L. Dutton, and F. Daldal (2000)
PNAS 97, 4567-4572
   Abstract »    Full Text »    PDF »
Definition of the Interaction Domain for Cytochrome c on the Cytochrome bc1 Complex. STEADY-STATE AND RAPID KINETIC ANALYSIS OF ELECTRON TRANSFER BETWEEN CYTOCHROME c AND RHODOBACTER SPHAEROIDES CYTOCHROME bc1 SURFACE MUTANTS.
H. Tian, R. Sadoski, L. Zhang, C.-A. Yu, L. Yu, B. Durham, and F. Millett (2000)
J. Biol. Chem. 275, 9587-9595
   Abstract »    Full Text »    PDF »
pH-induced Intramolecular Electron Transfer between the Iron-Sulfur Protein and Cytochrome c1 in Bovine Cytochrome bc1 Complex.
L. Zhang, C.-H. Tai, L. Yu, and C.-A. Yu (2000)
J. Biol. Chem. 275, 7656-7661
   Abstract »    Full Text »    PDF »
Membrane Topology and Insertion of Membrane Proteins: Search for Topogenic Signals.
M. van Geest and J. S. Lolkema (2000)
Microbiol. Mol. Biol. Rev. 64, 13-33
   Abstract »    Full Text »    PDF »
On the Spatial Organization of Hemes and Chlorophyll in Cytochrome b6 f. A LINEAR AND CIRCULAR DICHROISM STUDY.
B. Schoepp, E. Chabaud, C. Breyton, A. Vermeglio, and J.-L. Popot (2000)
J. Biol. Chem. 275, 5275-5283
   Abstract »    Full Text »    PDF »
Relative Orientation of the Hemes of the Cytochrome bc1 Complexes from Rhodobacter sphaeroides, Rhodospirillum rubrum, and Beef Heart Mitochondria. A LINEAR DICHROISM STUDY.
B. Schoepp, J. Breton, P. Parot, and A. Vermeglio (2000)
J. Biol. Chem. 275, 5284-5290
   Abstract »    Full Text »    PDF »
Structural details of an interaction between cardiolipin and an integral membrane protein.
K. E. McAuley, P. K. Fyfe, J. P. Ridge, N. W. Isaacs, R. J. Cogdell, and M. R. Jones (1999)
PNAS 96, 14706-14711
   Abstract »    Full Text »    PDF »
The FMN to Heme Electron Transfer in Cytochrome P450BM-3. EFFECT OF CHEMICAL MODIFICATION OF CYSTEINES ENGINEERED AT THE FMN-HEME DOMAIN INTERACTION SITE.
I. F. Sevrioukova, J. T. Hazzard, G. Tollin, and T. L. Poulos (1999)
J. Biol. Chem. 274, 36097-36106
   Abstract »    Full Text »    PDF »
Genes of the sbo-alb Locus of Bacillus subtilis Are Required for Production of the Antilisterial Bacteriocin Subtilosin.
G. Zheng, L. Z. Yan, J. C. Vederas, and P. Zuber (1999)
J. Bacteriol. 181, 7346-7355
   Abstract »    Full Text »
The Energy Landscape for Ubihydroquinone Oxidation at the Qo Site of the bc1 Complex in Rhodobacter sphaeroides.
S. Hong, N. Ugulava, M. Guergova-Kuras, and A. R. Crofts (1999)
J. Biol. Chem. 274, 33931-33944
   Abstract »    Full Text »    PDF »
H+-translocating NADH-Quinone Oxidoreductase (NDH-1) of Paracoccus denitrificans. STUDIES ON TOPOLOGY AND STOICHIOMETRY OF THE PERIPHERAL SUBUNITS.
T. Yano and T. Yagi (1999)
J. Biol. Chem. 274, 28606-28611
   Abstract »    Full Text »    PDF »
Analysis of the Nucleus-Encoded and Chloroplast-Targeted Rieske Protein by Classic and Site-Directed Mutagenesis of Chlamydomonas.
C. de Vitry, G. Finazzi, F. Baymann, and T. Kallas (1999)
PLANT CELL 11, 2031-2044
   Abstract »    Full Text »
Exercise Intolerance Due to Mutations in the Cytochrome b Gene of Mitochondrial DNA.
A. L. Andreu, M. G. Hanna, H. Reichmann, C. Bruno, A. S. Penn, K. Tanji, F. Pallotti, S. Iwata, E. Bonilla, B. Lach, et al. (1999)
N. Engl. J. Med. 341, 1037-1044
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)