Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 26 June 1998:
Vol. 280. no. 5372, pp. 2114 - 2118
DOI: 10.1126/science.280.5372.2114
Prev | Table of Contents | Next

Reports

Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic Escherichia coli

David Bieber, Sandra W. Ramer, Cheng-Yen Wu, William J. Murray, Toru Tobe, Rosemary Fernandez, Gary K. Schoolnik *

Type IV bundle-forming pili of enteropathogenic Escherichia coli are required for the localized adherence and autoaggregation phenotypes. Whether these pili are also required for virulence was tested in volunteers by inactivating bfpA or bfpT (perA) encoding, respectively, the pilus subunit and the bfp operon transcriptional activator. Both mutants caused significantly less diarrhea. Mutation of the bfpF nucleotide-binding domain caused increased piliation, enhanced localized adherence, and abolished the twitching motility-dispersal phase of the autoaggregation phenotype. The bfpF mutant colonized the human intestine but was about 200-fold less virulent. Thus, BfpF is required for dispersal from the bacterial aggregate and for full virulence.

D. Bieber, S. W. Ramer, C.-Y. Wu, R. Fernandez, G. K. Schoolnik, Department of Medicine, Division of Infectious Diseases and Geographical Medicine, and Department of Microbiology and Immunology, Stanford Program for Vaccine Research, Stanford University Medical Center, Stanford, CA 94305 USA.
W. J. Murray, Department of Biological Sciences, San Jose State University, San Jose, CA 95912 USA.
T. Tobe, Department of Bacteriology, Institute of Medicine, University of Tokyo, Tokyo, Japan.
*   To whom correspondence should be addressed. E-mail: ml.gks{at}forsythe.stanford.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Contribution of Conserved ATP-Dependent Proteases of Campylobacter jejuni to Stress Tolerance and Virulence.
M. T. Cohn, H. Ingmer, F. Mulholland, K. Jorgensen, J. M. Wells, and L. Brondsted (2007)
Appl. Envir. Microbiol. 73, 7803-7813
   Abstract »    Full Text »    PDF »
Effect of Zinc in Enteropathogenic Escherichia coli Infection.
J. K. Crane, T. M. Naeher, I. Shulgina, C. Zhu, and E. C. Boedeker (2007)
Infect. Immun. 75, 5974-5984
   Abstract »    Full Text »    PDF »
Contribution of Moraxella catarrhalis Type IV Pili to Nasopharyngeal Colonization and Biofilm Formation.
N. R. Luke, J. A. Jurcisek, L. O. Bakaletz, and A. A. Campagnari (2007)
Infect. Immun. 75, 5559-5564
   Abstract »    Full Text »    PDF »
3D structure/function analysis of PilX reveals how minor pilins can modulate the virulence properties of type IV pili.
S. Helaine, D. H. Dyer, X. Nassif, V. Pelicic, and K. T. Forest (2007)
PNAS 104, 15888-15893
   Abstract »    Full Text »    PDF »
Functional Consequences of Sequence Variation in Bundlin, the Enteropathogenic Escherichia coli Type IV Pilin Protein.
P. J. Fernandes, Q. Guo, and M. S. Donnenberg (2007)
Infect. Immun. 75, 4687-4696
   Abstract »    Full Text »    PDF »
Enteropathogenic and Enterohemorrhagic Escherichia coli Virulence Gene Regulation.
J. L. Mellies, A. M. S. Barron, and A. M. Carmona (2007)
Infect. Immun. 75, 4199-4210
   Full Text »    PDF »
A Second Large Plasmid Encodes Conjugative Transfer and Antimicrobial Resistance in O119:H2 and Some Typical O111 Enteropathogenic Escherichia coli Strains.
A. I. Nwaneshiudu, T. Mucci, D. J. Pickard, and I. N. Okeke (2007)
J. Bacteriol. 189, 6074-6079
   Abstract »    Full Text »    PDF »
Molecular Evolution of Typical Enteropathogenic Escherichia coli: Clonal Analysis by Multilocus Sequence Typing and Virulence Gene Allelic Profiling.
D. W. Lacher, H. Steinsland, T. E. Blank, M. S. Donnenberg, and T. S. Whittam (2007)
J. Bacteriol. 189, 342-350
   Abstract »    Full Text »    PDF »
MarA, SoxS and Rob function as virulence factors in an Escherichia coli murine model of ascending pyelonephritis.
P. Casaz, L. K. Garrity-Ryan, D. McKenney, C. Jackson, S. B. Levy, S. K. Tanaka, and M. N. Alekshun (2006)
Microbiology 152, 3643-3650
   Abstract »    Full Text »    PDF »
Prebiotic Galactooligosaccharides Reduce Adherence of Enteropathogenic Escherichia coli to Tissue Culture Cells.
K. Shoaf, G. L. Mulvey, G. D. Armstrong, and R. W. Hutkins (2006)
Infect. Immun. 74, 6920-6928
   Abstract »    Full Text »    PDF »
Identification of Virulence Genes Linked with Diarrhea Due to Atypical Enteropathogenic Escherichia coli by DNA Microarray Analysis and PCR..
J. E. Afset, G. Bruant, R. Brousseau, J. Harel, E. Anderssen, L. Bevanger, and K. Bergh (2006)
J. Clin. Microbiol. 44, 3703-3711
   Abstract »    Full Text »    PDF »
Temperature-Regulated Microcolony Formation by Burkholderia pseudomallei Requires pilA and Enhances Association with Cultured Human Cells.
J. A. Boddey, C. P. Flegg, C. J. Day, I. R. Beacham, and I. R. Peak (2006)
Infect. Immun. 74, 5374-5381
   Abstract »    Full Text »    PDF »
Nucleotide Sequence Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid pMAR7.
C. Brinkley, V. Burland, R. Keller, D. J. Rose, A. T. Boutin, S. A. Klink, F. R. Blattner, and J. B. Kaper (2006)
Infect. Immun. 74, 5408-5413
   Abstract »    Full Text »    PDF »
N-Acetyllactosamine Conjugated to Gold Nanoparticles Inhibits Enteropathogenic Escherichia coli Colonization of the Epithelium in Human Intestinal Biopsy Specimens.
R. M. Hyland, P. Beck, G. L. Mulvey, P. I. Kitov, and G. D. Armstrong (2006)
Infect. Immun. 74, 5419-5421
   Abstract »    Full Text »    PDF »
Interaction and localization studies of enteropathogenic Escherichia coli type IV bundle-forming pilus outer membrane components..
A. Daniel, A. Singh, L. J. Crowther, P. J. Fernandes, W. Schreiber, and M. S. Donnenberg (2006)
Microbiology 152, 2405-2420
   Abstract »    Full Text »    PDF »
FppA, a Novel Pseudomonas aeruginosa Prepilin Peptidase Involved in Assembly of Type IVb Pili.
S. de Bentzmann, M. Aurouze, G. Ball, and A. Filloux (2006)
J. Bacteriol. 188, 4851-4860
   Abstract »    Full Text »    PDF »
Antigen-43-mediated autoaggregation impairs motility in Escherichia coli.
G. C. Ulett, R. I. Webb, and M. A. Schembri (2006)
Microbiology 152, 2101-2110
   Abstract »    Full Text »    PDF »
Bundle-Forming Pili and EspA Are Involved in Biofilm Formation by Enteropathogenic Escherichia coli.
C. G. Moreira, K. Palmer, M. Whiteley, M. P. Sircili, L. R. Trabulsi, A. F. P. Castro, and V. Sperandio (2006)
J. Bacteriol. 188, 3952-3961
   Abstract »    Full Text »    PDF »
Basis for N-acetyllactosamine-mediated inhibition of enteropathogenic Escherichia coli localized adherence.
R. M. Hyland, T. P. Griener, G. L. Mulvey, P. I. Kitov, O. P. Srivastava, P. Marcato, and G. D. Armstrong (2006)
J. Med. Microbiol. 55, 669-675
   Abstract »    Full Text »    PDF »
Mutual Enhancement of Virulence by Enterotoxigenic and Enteropathogenic Escherichia coli.
J. K. Crane, S. S. Choudhari, T. M. Naeher, and M. E. Duffey (2006)
Infect. Immun. 74, 1505-1515
   Abstract »    Full Text »    PDF »
AggA is required for aggregation and increased biofilm formation of a hyper-aggregating mutant of Shewanella oneidensis MR-1..
W. De Windt, H. Gao, W. Kromer, P. Van Damme, J. Dick, J. Mast, N. Boon, J. Zhou, and W. Verstraete (2006)
Microbiology 152, 721-729
   Abstract »    Full Text »    PDF »
Nanoscale Characterization and Determination of Adhesion Forces of Pseudomonas aeruginosa Pili by Using Atomic Force Microscopy.
A. Touhami, M. H. Jericho, J. M. Boyd, and T. J. Beveridge (2006)
J. Bacteriol. 188, 370-377
   Abstract »    Full Text »    PDF »
The Global Regulator Ler Is Necessary for Enteropathogenic Escherichia coli Colonization of Caenorhabditis elegans.
J. L. Mellies, A. M. S. Barron, K. R. Haack, A. S. Korson, and D. A. Oldridge (2006)
Infect. Immun. 74, 64-72
   Abstract »    Full Text »    PDF »
Structure of the Bundle-forming Pilus from Enteropathogenic Escherichia coli.
S. Ramboarina, P. J. Fernandes, S. Daniell, S. Islam, P. Simpson, G. Frankel, F. Booy, M. S. Donnenberg, and S. Matthews (2005)
J. Biol. Chem. 280, 40252-40260
   Abstract »    Full Text »    PDF »
Aptamers That Preferentially Bind Type IVB Pili and Inhibit Human Monocytic-Cell Invasion by Salmonella enterica Serovar Typhi.
Q. Pan, X.-L. Zhang, H.-Y. Wu, P.-W. He, F. Wang, M.-S. Zhang, J.-M. Hu, B. Xia, and J. Wu (2005)
Antimicrob. Agents Chemother. 49, 4052-4060
   Abstract »    Full Text »    PDF »
Interaction of Enteropathogenic and Shiga Toxin-Producing Escherichia coli and Porcine Intestinal Mucosa: Role of Intimin and Tir in Adherence.
F. Girard, I. Batisson, G. M. Frankel, J. Harel, and J. M. Fairbrother (2005)
Infect. Immun. 73, 6005-6016
   Abstract »    Full Text »    PDF »
Two pathways for ATP release from host cells in enteropathogenic Escherichia coli infection.
J. K. Crane, T. M. Naeher, S. S. Choudhari, and E. M. Giroux (2005)
Am J Physiol Gastrointest Liver Physiol 289, G407-G417
   Abstract »    Full Text »    PDF »
The ATPase Activity of BfpD Is Greatly Enhanced by Zinc and Allosteric Interactions with Other Bfp Proteins.
L. J. Crowther, A. Yamagata, L. Craig, J. A. Tainer, and M. S. Donnenberg (2005)
J. Biol. Chem. 280, 24839-24848
   Abstract »    Full Text »    PDF »
The HtrA Protease of Campylobacter jejuni Is Required for Heat and Oxygen Tolerance and for Optimal Interaction with Human Epithelial Cells.
L. Brondsted, M. T. Andersen, M. Parker, K. Jorgensen, and H. Ingmer (2005)
Appl. Envir. Microbiol. 71, 3205-3212
   Abstract »    Full Text »    PDF »
The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and Biofilm Formation.
O. Sherlock, R. M. Vejborg, and P. Klemm (2005)
Infect. Immun. 73, 1954-1963
   Abstract »    Full Text »    PDF »
The StcE Protease Contributes to Intimate Adherence of Enterohemorrhagic Escherichia coli O157:H7 to Host Cells.
T. E. Grys, M. B. Siegel, W. W. Lathem, and R. A. Welch (2005)
Infect. Immun. 73, 1295-1303
   Abstract »    Full Text »    PDF »
A Vibrio vulnificus Type IV Pilin Contributes to Biofilm Formation, Adherence to Epithelial Cells, and Virulence.
R. N. Paranjpye and M. S. Strom (2005)
Infect. Immun. 73, 1411-1422
   Abstract »    Full Text »    PDF »
Interaction of Enteropathogenic Escherichia coli with Human Intestinal Mucosa: Role of Effector Proteins in Brush Border Remodeling and Formation of Attaching and Effacing Lesions.
R. K Shaw, J. Cleary, M. S. Murphy, G. Frankel, and S. Knutton (2005)
Infect. Immun. 73, 1243-1251
   Abstract »    Full Text »    PDF »
Functional Dissection of a Conserved Motif within the Pilus Retraction Protein PilT.
K. G. Aukema, E. M. Kron, T. J. Herdendorf, and K. T. Forest (2005)
J. Bacteriol. 187, 611-618
   Abstract »    Full Text »    PDF »
The Cpx Envelope Stress Response Affects Expression of the Type IV Bundle-Forming Pili of Enteropathogenic Escherichia coli.
A. Z. Nevesinjac and T. L. Raivio (2005)
J. Bacteriol. 187, 672-686
   Abstract »    Full Text »    PDF »
Adherence of Diarrheagenic Escherichia coli Strains to Epithelial Cells.
A. G. Torres, X. Zhou, and J. B. Kaper (2005)
Infect. Immun. 73, 18-29
   Full Text »    PDF »
Inactivation of Escherichia coli O157:H7 in Simulated Human Gastric Fluid.
M. L. Tamplin (2005)
Appl. Envir. Microbiol. 71, 320-325
   Abstract »    Full Text »    PDF »
Novel Roles for the AIDA Adhesin from Diarrheagenic Escherichia coli: Cell Aggregation and Biofilm Formation.
O. Sherlock, M. A. Schembri, A. Reisner, and P. Klemm (2004)
J. Bacteriol. 186, 8058-8065
   Abstract »    Full Text »    PDF »
Association of atypical enteropathogenic Escherichia coli (EPEC) with prolonged diarrhoea.
J. E Afset, L. Bevanger, P. Romundstad, and K. Bergh (2004)
J. Med. Microbiol. 53, 1137-1144
   Abstract »    Full Text »    PDF »
Two Morphological Types of Cell Appendages on a Strongly Adhesive Bacterium, Acinetobacter sp. Strain Tol 5.
S. Ishii, J. Koki, H. Unno, and K. Hori (2004)
Appl. Envir. Microbiol. 70, 5026-5029
   Abstract »    Full Text »    PDF »
A force-dependent switch reverses type IV pilus retraction.
B. Maier, M. Koomey, and M. P. Sheetz (2004)
PNAS 101, 10961-10966
   Abstract »    Full Text »    PDF »
NMR Structure of a Type IVb Pilin from Salmonella typhi and Its Assembly into Pilus.
X.-F. Xu, Y.-W. Tan, L. Lam, J. Hackett, M. Zhang, and Y.-K. Mok (2004)
J. Biol. Chem. 279, 31599-31605
   Abstract »    Full Text »    PDF »
Contribution of Long Polar Fimbriae to the Virulence of Rabbit-Specific Enteropathogenic Escherichia coli.
H. J. Newton, J. Sloan, V. Bennett-Wood, L. M. Adams, R. M. Robins-Browne, and E. L. Hartland (2004)
Infect. Immun. 72, 1230-1239
   Abstract »    Full Text »    PDF »
Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal epithelial cells: role of bundle-forming pili (BFP), EspA filaments and intimin.
J. Cleary, L.-C. Lai, R. K. Shaw, A. Straatman-Iwanowska, M. S. Donnenberg, G. Frankel, and S. Knutton (2004)
Microbiology 150, 527-538
   Abstract »    Full Text »    PDF »
Legionella pneumophila Type II Protein Secretion Promotes Virulence in the A/J Mouse Model of Legionnaires' Disease Pneumonia.
O. Rossier, S. R. Starkenburg, and N. P. Cianciotto (2004)
Infect. Immun. 72, 310-321
   Abstract »    Full Text »    PDF »
Structural and Topographical Studies of the Type IV Bundle-Forming Pilus Assembly Complex of Enteropathogenic Escherichia coli.
J. Hwang, D. Bieber, S. W. Ramer, C.-Y. Wu, and G. K. Schoolnik (2003)
J. Bacteriol. 185, 6695-6701
   Abstract »    Full Text »    PDF »
Virulence of Enteropathogenic Escherichia coli, a Global Pathogen.
S. C. Clarke, R. D. Haigh, P. P. E. Freestone, and P. H. Williams (2003)
Clin. Microbiol. Rev. 16, 365-378
   Abstract »    Full Text »    PDF »
Identification of the DNA Binding Sites of PerA, the Transcriptional Activator of the bfp and per Operons in Enteropathogenic Escherichia coli.
J. A. Ibarra, M. I. Villalba, and J. L. Puente (2003)
J. Bacteriol. 185, 2835-2847
   Abstract »    Full Text »    PDF »
Highly adherent small-colony variants of Pseudomonas aeruginosa in cystic fibrosis lung infection.
S. Haussler, I. Ziegler, A. Lottel, F. v. Gotz, M. Rohde, D. Wehmhohner, S. Saravanamuthu, B. Tummler, and I. Steinmetz (2003)
J. Med. Microbiol. 52, 295-301
   Abstract »    Full Text »    PDF »
Role of Adhesin Release for Mucosal Colonization by a Bacterial Pathogen.
L. Coutte, S. Alonso, N. Reveneau, E. Willery, B. Quatannens, C. Locht, and F. Jacob-Dubuisson (2003)
J. Exp. Med. 197, 735-742
   Abstract »    Full Text »    PDF »
The Shufflon of Salmonella enterica Serovar Typhi Regulates Type IVB Pilus-Mediated Bacterial Self-Association.
C. Morris, C. M. C. Yip, I. S. M. Tsui, D. K.-H. Wong, and J. Hackett (2003)
Infect. Immun. 71, 1141-1146
   Abstract »    Full Text »    PDF »
DsbA of Pseudomonas aeruginosa Is Essential for Multiple Virulence Factors.
U.-H. Ha, Y. Wang, and S. Jin (2003)
Infect. Immun. 71, 1590-1595
   Abstract »    Full Text »    PDF »
A novel ColV plasmid encoding type IV pili.
U. Gophna, A. Parket, J. Hacker, and E. Z. Ron (2003)
Microbiology 149, 177-184
   Abstract »    Full Text »    PDF »
Yersinia pseudotuberculosis Harbors a Type IV Pilus Gene Cluster That Contributes to Pathogenicity.
F. Collyn, M.-A. Lety, S. Nair, V. Escuyer, A. Ben Younes, M. Simonet, and M. Marceau (2002)
Infect. Immun. 70, 6196-6205
   Abstract »    Full Text »    PDF »
Identification and Characterization of lpfABCC'DE, a Fimbrial Operon of Enterohemorrhagic Escherichia coli O157:H7.
A. G. Torres, J. A. Giron, N. T. Perna, V. Burland, F. R. Blattner, F. Avelino-Flores, and J. B. Kaper (2002)
Infect. Immun. 70, 5416-5427
   Abstract »    Full Text »    PDF »
Modification of Type IV Pilus-Associated Epithelial Cell Adherence and Multicellular Behavior by the PilU Protein of Neisseria gonorrhoeae.
H.-S. M. Park, M. Wolfgang, and M. Koomey (2002)
Infect. Immun. 70, 3891-3903
   Abstract »    Full Text »    PDF »
The Type IV Pilus Assembly Complex: Biogenic Interactions among the Bundle-Forming Pilus Proteins of Enteropathogenic Escherichia coli.
S. W. Ramer, G. K. Schoolnik, C.-Y. Wu, J. Hwang, S. A. Schmidt, and D. Bieber (2002)
J. Bacteriol. 184, 3457-3465
   Abstract »    Full Text »    PDF »
Release of ATP during host cell killing by enteropathogenic E. coli and its role as a secretory mediator.
J. K. Crane, R. A. Olson, H. M. Jones, and M. E. Duffey (2002)
Am J Physiol Gastrointest Liver Physiol 283, G74-G86
   Abstract »    Full Text »    PDF »
Characterization of a Novel Type IV Pilus Locus Encoded on the Large Plasmid of Locus of Enterocyte Effacement-Negative Shiga-Toxigenic Escherichia coli Strains That Are Virulent for Humans.
P. Srimanote, A. W. Paton, and J. C. Paton (2002)
Infect. Immun. 70, 3094-3100
   Abstract »    Full Text »    PDF »
Expression of the Bundle-Forming Pilus by Enteropathogenic Escherichia coli Strains of Heterologous Serotypes.
J. Gismero-Ordonez, M. Dall'Agnol, L. R. Trabulsi, and J. A. Giron (2002)
J. Clin. Microbiol. 40, 2291-2296
   Abstract »    Full Text »    PDF »
Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli.
R. Keller, J. G. Ordonez, R. R. de Oliveira, L. R. Trabulsi, T. J. Baldwin, and S. Knutton (2002)
Infect. Immun. 70, 2681-2689
   Abstract »    Full Text »    PDF »
Type IV Pilus-Dependent Motility and Its Possible Role in Bacterial Pathogenesis.
W. Shi and H. Sun (2002)
Infect. Immun. 70, 1-4
   Full Text »    PDF »
Neisseria gonorrhoeae PilV, a type IV pilus-associated protein essential to human epithelial cell adherence.
H. C. Winther-Larsen, F. T. Hegge, M. Wolfgang, S. F. Hayes, J. P. M. van Putten, and M. Koomey (2001)
PNAS 98, 15276-15281
   Abstract »    Full Text »    PDF »
Induction of Epithelial Cell Death Including Apoptosis by Enteropathogenic Escherichia coli Expressing Bundle-Forming Pili.
M. Abul-Milh, Y. Wu, B. Lau, C. A. Lingwood, and D. B. Foster (2001)
Infect. Immun. 69, 7356-7364
   Abstract »    Full Text »    PDF »
Enteropathogenic Escherichiacoli Virulence Factor Bundle-Forming Pilus Has a Binding Specificity for Phosphatidylethanolamine.
C. Khursigara, M. Abul-Milh, B. Lau, J. A. Giron, C. A. Lingwood, and D. E. B. Foster (2001)
Infect. Immun. 69, 6573-6579
   Abstract »    Full Text »    PDF »
Locus of Enterocyte Effacement from Citrobacter rodentium: Sequence Analysis and Evidence for Horizontal Transfer among Attaching and Effacing Pathogens.
W. Deng, Y. Li, B. A. Vallance, and B. B. Finlay (2001)
Infect. Immun. 69, 6323-6335
   Abstract »    Full Text »    PDF »
Comparative Sequence Analysis of the Plasmid-Encoded Regulator of Enteropathogenic Escherichia coli Strains.
I. N. Okeke, J. A. Borneman, S. Shin, J. L. Mellies, L. E. Quinn, and J. B. Kaper (2001)
Infect. Immun. 69, 5553-5564
   Abstract »    Full Text »    PDF »
Requirement of Novel Competence Genes pilT and pilU of Pseudomonas stutzeri for Natural Transformation and Suppression of pilT Deficiency by a Hexahistidine Tag on the Type IV Pilus Protein PilAI.
S. Graupner, N. Weger, M. Sohni, and W. Wackernagel (2001)
J. Bacteriol. 183, 4694-4701
   Abstract »    Full Text »    PDF »
Structure-Function Analysis of BfpB, a Secretin-Like Protein Encoded by the Bundle-Forming-Pilus Operon of Enteropathogenic Escherichia coli.
S. A. Schmidt, D. Bieber, S. W. Ramer, J. Hwang, C.-Y. Wu, and G. Schoolnik (2001)
J. Bacteriol. 183, 4848-4859
   Abstract »    Full Text »    PDF »
Novel Topology of BfpE, a Cytoplasmic Membrane Protein Required for Type IV Fimbrial Biogenesis in Enteropathogenic Escherichia coli.
T. E. Blank and M. S. Donnenberg (2001)
J. Bacteriol. 183, 4435-4450
   Abstract »    Full Text »    PDF »
Light regulation of type IV pilus-dependent motility by chemosensor-like elements in Synechocystis PCC6803.
D. Bhaya, A. Takahashi, and A. R. Grossman (2001)
PNAS
   Abstract »    Full Text »    PDF »
Pseudomonas aeruginosa Exhibits Directed Twitching Motility Up Phosphatidylethanolamine Gradients.
D. B. Kearns, J. Robinson, and L. J. Shimkets (2001)
J. Bacteriol. 183, 763-767
   Abstract »    Full Text »
Role of the Eikenella corrodens pilA Locus in Pilus Function and Phase Variation.
M. T. Villar, R. L. Hirschberg, and M. R. Schaefer (2001)
J. Bacteriol. 183, 55-62
   Abstract »    Full Text »
Molecular Variation among Type IV Pilin (bfpA) Genes from Diverse Enteropathogenic Escherichia coli Strains.
T. E. Blank, H. Zhong, A. L. Bell, T. S. Whittam, and M. S. Donnenberg (2000)
Infect. Immun. 68, 7028-7038
   Abstract »    Full Text »    PDF »
The Locus of Enterocyte Effacement (LEE)-Encoded Regulator Controls Expression of Both LEE- and Non-LEE-Encoded Virulence Factors in Enteropathogenic and Enterohemorrhagic Escherichia coli.
S. J. Elliott, V. Sperandio, J. A. Giron, S. Shin, J. L. Mellies, L. Wainwright, S. W. Hutcheson, T. K. McDaniel, and J. B. Kaper (2000)
Infect. Immun. 68, 6115-6126
   Abstract »    Full Text »    PDF »
Contribution of Plasmid-Encoded Fimbriae and Intimin to Capacity of Rabbit-Specific Enteropathogenic Escherichia coli To Attach to and Colonize Rabbit Intestine.
E. O. Krejany, T. H. Grant, V. Bennett-Wood, L. M. Adams, and R. M. Robins-Browne (2000)
Infect. Immun. 68, 6472-6477
   Abstract »    Full Text »    PDF »
Saccharomyces boulardii Preserves the Barrier Function and Modulates the Signal Transduction Pathway Induced in Enteropathogenic Escherichia coli-Infected T84 Cells.
D. Czerucka, S. Dahan, B. Mograbi, B. Rossi, and P. Rampal (2000)
Infect. Immun. 68, 5998-6004
   Abstract »    Full Text »    PDF »
Recognition of Enteropathogenic Escherichia coli Virulence Determinants by Human Colostrum and Serum Antibodies.
A. Parissi-Crivelli, J. M. Parissi-Crivelli, and J. A. Girón (2000)
J. Clin. Microbiol. 38, 2696-2700
   Abstract »    Full Text »
Salmonella enterica Serovar Typhi Uses Type IVB Pili To Enter Human Intestinal Epithelial Cells.
X.-L. Zhang, I. S. M. Tsui, C. M. C. Yip, A. W. Y. Fung, D. K.-H. Wong, X. Dai, Y. Yang, J. Hackett, and C. Morris (2000)
Infect. Immun. 68, 3067-3073
   Abstract »    Full Text »    PDF »
Role of EspB in Experimental Human Enteropathogenic Escherichia coli Infection.
C. O. Tacket, M. B. Sztein, G. Losonsky, A. Abe, B. B. Finlay, B. P. McNamara, G. T. Fantry, S. P. James, J. P. Nataro, M. M. Levine, et al. (2000)
Infect. Immun. 68, 3689-3695
   Abstract »    Full Text »    PDF »
Curli Loci of Shigella spp..
H. Sakellaris, N. K. Hannink, K. Rajakumar, D. Bulach, M. Hunt, C. Sasakawa, and B. Adler (2000)
Infect. Immun. 68, 3780-3783
   Abstract »    Full Text »    PDF »
Effects of bfp Mutations on Biogenesis of Functional Enteropathogenic Escherichia coli Type IV Pili.
R. P. Anantha, K. D. Stone, and M. S. Donnenberg (2000)
J. Bacteriol. 182, 2498-2506
   Abstract »    Full Text »
Role of Tir and Intimin in the Virulence of Rabbit Enteropathogenic Escherichia coli Serotype O103:H2.
O. Marches, J.-P. Nougayrede, S. Boullier, J. Mainil, G. Charlier, I. Raymond, P. Pohl, M. Boury, J. De Rycke, A. Milon, et al. (2000)
Infect. Immun. 68, 2171-2182
   Abstract »    Full Text »    PDF »
Evidence for specificity in type 4 pilus biogenesis by enteropathogenic Escherichia coli.
B. P. McNamara and M. S. Donnenberg (2000)
Microbiology 146, 719-729
   Abstract »    Full Text »
PpdD Type IV Pilin of Escherichia coli K-12 Can Be Assembled into Pili in Pseudomonas aeruginosa.
N. Sauvonnet, P. Gounon, and A. P. Pugsley (2000)
J. Bacteriol. 182, 848-