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Science 26 June 1998:
Vol. 280. no. 5372, pp. 2114 - 2118
DOI: 10.1126/science.280.5372.2114
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Reports

Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic Escherichia coli

David Bieber, Sandra W. Ramer, Cheng-Yen Wu, William J. Murray, Toru Tobe, Rosemary Fernandez, Gary K. Schoolnik *

Type IV bundle-forming pili of enteropathogenic Escherichia coli are required for the localized adherence and autoaggregation phenotypes. Whether these pili are also required for virulence was tested in volunteers by inactivating bfpA or bfpT (perA) encoding, respectively, the pilus subunit and the bfp operon transcriptional activator. Both mutants caused significantly less diarrhea. Mutation of the bfpF nucleotide-binding domain caused increased piliation, enhanced localized adherence, and abolished the twitching motility-dispersal phase of the autoaggregation phenotype. The bfpF mutant colonized the human intestine but was about 200-fold less virulent. Thus, BfpF is required for dispersal from the bacterial aggregate and for full virulence.

D. Bieber, S. W. Ramer, C.-Y. Wu, R. Fernandez, G. K. Schoolnik, Department of Medicine, Division of Infectious Diseases and Geographical Medicine, and Department of Microbiology and Immunology, Stanford Program for Vaccine Research, Stanford University Medical Center, Stanford, CA 94305 USA.
W. J. Murray, Department of Biological Sciences, San Jose State University, San Jose, CA 95912 USA.
T. Tobe, Department of Bacteriology, Institute of Medicine, University of Tokyo, Tokyo, Japan.
*   To whom correspondence should be addressed. E-mail: ml.gks{at}forsythe.stanford.edu

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Infect. Immun. 68, 6472-6477
   Abstract »    Full Text »    PDF »
Saccharomyces boulardii Preserves the Barrier Function and Modulates the Signal Transduction Pathway Induced in Enteropathogenic Escherichia coli-Infected T84 Cells.
D. Czerucka, S. Dahan, B. Mograbi, B. Rossi, and P. Rampal (2000)
Infect. Immun. 68, 5998-6004
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Recognition of Enteropathogenic Escherichia coli Virulence Determinants by Human Colostrum and Serum Antibodies.
A. Parissi-Crivelli, J. M. Parissi-Crivelli, and J. A. Girón (2000)
J. Clin. Microbiol. 38, 2696-2700
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Salmonella enterica Serovar Typhi Uses Type IVB Pili To Enter Human Intestinal Epithelial Cells.
X.-L. Zhang, I. S. M. Tsui, C. M. C. Yip, A. W. Y. Fung, D. K.-H. Wong, X. Dai, Y. Yang, J. Hackett, and C. Morris (2000)
Infect. Immun. 68, 3067-3073
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Role of EspB in Experimental Human Enteropathogenic Escherichia coli Infection.
C. O. Tacket, M. B. Sztein, G. Losonsky, A. Abe, B. B. Finlay, B. P. McNamara, G. T. Fantry, S. P. James, J. P. Nataro, M. M. Levine, et al. (2000)
Infect. Immun. 68, 3689-3695
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Curli Loci of Shigella spp..
H. Sakellaris, N. K. Hannink, K. Rajakumar, D. Bulach, M. Hunt, C. Sasakawa, and B. Adler (2000)
Infect. Immun. 68, 3780-3783
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Effects of bfp Mutations on Biogenesis of Functional Enteropathogenic Escherichia coli Type IV Pili.
R. P. Anantha, K. D. Stone, and M. S. Donnenberg (2000)
J. Bacteriol. 182, 2498-2506
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Role of Tir and Intimin in the Virulence of Rabbit Enteropathogenic Escherichia coli Serotype O103:H2.
O. Marches, J.-P. Nougayrede, S. Boullier, J. Mainil, G. Charlier, I. Raymond, P. Pohl, M. Boury, J. De Rycke, A. Milon, et al. (2000)
Infect. Immun. 68, 2171-2182
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Evidence for specificity in type 4 pilus biogenesis by enteropathogenic Escherichia coli.
B. P. McNamara and M. S. Donnenberg (2000)
Microbiology 146, 719-729
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PpdD Type IV Pilin of Escherichia coli K-12 Can Be Assembled into Pili in Pseudomonas aeruginosa.
N. Sauvonnet, P. Gounon, and A. P. Pugsley (2000)
J. Bacteriol. 182, 848-854
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The Type 4 Prepilin Peptidases Comprise a Novel Family of Aspartic Acid Proteases.
C. F. LaPointe and R. K. Taylor (2000)
J. Biol. Chem. 275, 1502-1510
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Citrobacter rodentium espB Is Necessary for Signal Transduction and for Infection of Laboratory Mice.
J. V. Newman, B. A. Zabel, S. S. Jha, and D. B. Schauer (1999)
Infect. Immun. 67, 6019-6025
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Complete DNA Sequence and Structural Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid.
T. Tobe, T. Hayashi, C.-G. Han, G. K. Schoolnik, E. Ohtsubo, and C. Sasakawa (1999)
Infect. Immun. 67, 5455-5462
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The 102-Kilobase pgm Locus of Yersinia pestis: Sequence Analysis and Comparison of Selected Regions among Different Yersinia pestis and Yersinia pseudotuberculosis Strains.
C. Buchrieser, C. Rusniok, L. Frangeul, E. Couve, A. Billault, F. Kunst, E. Carniel, and P. Glaser (1999)
Infect. Immun. 67, 4851-4861
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Eikenella corrodens Phase Variation Involves a Posttranslational Event in Pilus Formation.
M. T. Villar, J. T. Helber, B. Hood, M. R. Schaefer, and R. L. Hirschberg (1999)
J. Bacteriol. 181, 4154-4160
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Pili Binding to Asialo-GM1 on Epithelial Cells Can Mediate Cytotoxicity or Bacterial Internalization by Pseudomonas aeruginosa.
J. C. Comolli, L. L. Waite, K. E. Mostov, and J. N. Engel (1999)
Infect. Immun. 67, 3207-3214
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Pseudomonas aeruginosa Gene Products PilT and PilU Are Required for Cytotoxicity In Vitro and Virulence in a Mouse Model of Acute Pneumonia.
J. C. Comolli, A. R. Hauser, L. Waite, C. B. Whitchurch, J. S. Mattick, and J. N. Engel (1999)
Infect. Immun. 67, 3625-3630
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