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Science 12 June 1998:
Vol. 280. no. 5370, pp. 1760 - 1763
DOI: 10.1126/science.280.5370.1760
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Reports

The Ubiquitin-Related Protein RUB1 and Auxin Response in Arabidopsis

J. C. del Pozo, C. Timpte, * S. Tan, J. Callis, M. Estelle dagger

The AXR1 (auxin-resistant) protein, which has features of the ubiquitin-activating enzyme E1, is required for normal response to the plant hormone auxin in Arabidopsis thaliana. ECR1 functions together with AXR1 to activate members of the RUB/NEDD8 family of ubiquitin-related proteins. Extracts from mutant seedlings lacking AXR1 did not promote formation of the RUB-ECR1 thiolester, indicating that AXR1 is the major activity in this tissue. AXR1 was localized primarily to the nucleus of dividing and elongating cells, suggesting that the targets of RUB modification are nuclear. These results indicate that auxin response depends on RUB modification of one or more nuclear proteins.

J. C. del Pozo, C. Timpte, M. Estelle, Department of Biology, Indiana University, Bloomington, IN 47405-6801, USA.
S. Tan and J. Callis, Section of Molecular and Cellular Biology, University of California-Davis, 1 Shields Avenue, Davis, CA 95616, USA.
*   Present address: Department of Biological Sciences, University of New Orleans, New Orleans, LA 70148, USA.

dagger    To whom correspondence should be addressed. E-mail: mestelle{at}bio.indiana.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)