Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 May 1998:
Vol. 280. no. 5365, pp. 909 - 912
DOI: 10.1126/science.280.5365.909
Prev | Table of Contents | Next

Reports

Replication Checkpoint Enforced by Kinases Cds1 and Chk1

Michael N. Boddy, Beth Furnari, Odile Mondesert, Paul Russell *

Cdc2, the kinase that induces mitosis, is regulated by checkpoints that couple mitosis to the completion of DNA replication and repair. The repair checkpoint kinase Chk1 regulates Cdc25, a phosphatase that activates Cdc2. Effectors of the replication checkpoint evoked by hydroxyurea (HU) are unknown. Treatment of fission yeast with HU stimulated the kinase Cds1, which appears to phosphorylate the kinase Wee1, an inhibitor of Cdc2. The protein kinase Cds1 was also required for a large HU-induced increase in the amount of Mik1, a second inhibitor of Cdc2. HU-induced arrest of cell division was abolished in cds1 chk1 cells. Thus, Cds1 and Chk1 appear to jointly enforce the replication checkpoint.

Departments of Molecular Biology and Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
*   To whom correspondence should be addressed. E-mail: prussell{at}scripps.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Cds1-Mediated Checkpoint Protects the MBF Activator Rep2 from Ubiquitination by Anaphase-Promoting Complex/Cyclosome-Ste9 at S-Phase Arrest in Fission Yeast.
Z. Chu, M. Eshaghi, S. Y. Poon, and J. Liu (2009)
Mol. Cell. Biol. 29, 4959-4970
   Abstract »    Full Text »    PDF »
Fission yeast Ku protein is required for recovery from DNA replication stress.
T. Miyoshi, J. Kanoh, and F. Ishikawa (2009)
Genes Cells 14, 1091-1103
   Abstract »    Full Text »    PDF »
Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA Repair.
A. Pedram, M. Razandi, A. J. Evinger, E. Lee, and E. R. Levin (2009)
Mol. Biol. Cell 20, 3374-3389
   Abstract »    Full Text »    PDF »
Autoinhibition and Autoactivation of the DNA Replication Checkpoint Kinase Cds1.
Y.-j. Xu and T. J. Kelly (2009)
J. Biol. Chem. 284, 16016-16027
   Abstract »    Full Text »    PDF »
The Role of MRN in the S-Phase DNA Damage Checkpoint Is Independent of Its Ctp1-dependent Roles in Double-Strand Break Repair and Checkpoint Signaling.
M. E. Porter-Goff and N. Rhind (2009)
Mol. Biol. Cell 20, 2096-2107
   Abstract »    Full Text »    PDF »
Mcm4 C-terminal domain of MCM helicase prevents excessive formation of single-stranded DNA at stalled replication forks.
N. Nitani, C. Yadani, H. Yabuuchi, H. Masukata, and T. Nakagawa (2008)
PNAS 105, 12973-12978
   Abstract »    Full Text »    PDF »
DNA replication checkpoint promotes G1-S transcription by inactivating the MBF repressor Nrm1.
R. A. M. de Bruin, T. I. Kalashnikova, A. Aslanian, J. Wohlschlegel, C. Chahwan, J. R. Yates III, P. Russell, and C. Wittenberg (2008)
PNAS 105, 11230-11235
   Abstract »    Full Text »    PDF »
Expanded Roles for Chk1 in Genome Maintenance.
G. H. Enders (2008)
J. Biol. Chem. 283, 17749-17752
   Abstract »    Full Text »    PDF »
Involvement of fission yeast Clr6-HDAC in regulation of the checkpoint kinase Cds1.
T. Kunoh, T. Habu, and T. Matsumoto (2008)
Nucleic Acids Res. 36, 3311-3319
   Abstract »    Full Text »    PDF »
Cds1 Controls the Release of Cdc14-like Phosphatase Flp1 from the Nucleolus to Drive Full Activation of the Checkpoint Response to Replication Stress in Fission Yeast.
H. Diaz-Cuervo and A. Bueno (2008)
Mol. Biol. Cell 19, 2488-2499
   Abstract »    Full Text »    PDF »
Human Immunodeficiency Virus Type 1 Vpr Induces Cell Cycle G2 Arrest through Srk1/MK2-Mediated Phosphorylation of Cdc25.
S. Huard, R. T. Elder, D. Liang, G. Li, and R. Y. Zhao (2008)
J. Virol. 82, 2904-2917
   Abstract »    Full Text »    PDF »
Minichromosome Maintenance Proteins Interact with Checkpoint and Recombination Proteins To Promote S-Phase Genome Stability.
J. M. Bailis, D. D. Luche, T. Hunter, and S. L. Forsburg (2008)
Mol. Cell. Biol. 28, 1724-1738
   Abstract »    Full Text »    PDF »
Cleavage of Stalled Forks by Fission Yeast Mus81/Eme1 in Absence of DNA Replication Checkpoint.
B. Froget, J. Blaisonneau, S. Lambert, and G. Baldacci (2008)
Mol. Biol. Cell 19, 445-456
   Abstract »    Full Text »    PDF »
RFCCtf18 and the Swi1-Swi3 Complex Function in Separate and Redundant Pathways Required for the Stabilization of Replication Forks to Facilitate Sister Chromatid Cohesion in Schizosaccharomyces pombe.
A. B. Ansbach, C. Noguchi, I. W. Klansek, M. Heidlebaugh, T. M. Nakamura, and E. Noguchi (2008)
Mol. Biol. Cell 19, 595-607
   Abstract »    Full Text »    PDF »
Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast.
N. Fersht, D. Hermand, J. Hayles, and P. Nurse (2007)
Nucleic Acids Res. 35, 5323-5337
   Abstract »    Full Text »    PDF »
Reducing DNA Polymerase {alpha} in the Absence of Drosophila ATR Leads to P53-Dependent Apoptosis and Developmental Defects.
J. R. LaRocque, D. L. Dougherty, S. K. Hussain, and J. Sekelsky (2007)
Genetics 176, 1441-1451
   Abstract »    Full Text »    PDF »
Modulation of Cell Cycle-specific Gene Expressions at the Onset of S Phase Arrest Contributes to the Robust DNA Replication Checkpoint Response in Fission Yeas.
Z. Chu, J. Li, M. Eshaghi, X. Peng, R. K. M. Karuturi, and J. Liu (2007)
Mol. Biol. Cell 18, 1756-1767
   Abstract »    Full Text »    PDF »
Drosophila ATR in Double-Strand Break Repair.
J. R. LaRocque, B. Jaklevic, T. T. Su, and J. Sekelsky (2007)
Genetics 175, 1023-1033
   Abstract »    Full Text »    PDF »
Caf1 regulates translocation of ribonucleotide reductase by releasing nucleoplasmic Spd1-Suc22 assembly.
S. Takahashi, K. Kontani, Y. Araki, and T. Katada (2007)
Nucleic Acids Res. 35, 1187-1197
   Abstract »    Full Text »    PDF »
Tel2 Is Required for Activation of the Mrc1-mediated Replication Checkpoint.
M. Shikata, F. Ishikawa, and J. Kanoh (2007)
J. Biol. Chem. 282, 5346-5355
   Abstract »    Full Text »    PDF »
Sap1 Promotes the Association of the Replication Fork Protection Complex With Chromatin and Is Involved in the Replication Checkpoint in Schizosaccharomyces pombe.
C. Noguchi and E. Noguchi (2007)
Genetics 175, 553-566
   Abstract »    Full Text »    PDF »
Arabidopsis WEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint.
K. De Schutter, J. Joubes, T. Cools, A. Verkest, F. Corellou, E. Babiychuk, E. Van Der Schueren, T. Beeckman, S. Kushnir, D. Inze, et al. (2007)
PLANT CELL 19, 211-225
   Abstract »    Full Text »    PDF »
Checkpoint proteins control morphogenetic events during DNA replication stress in Saccharomyces cerevisiae.
J. M. Enserink, M. B. Smolka, H. Zhou, and R. D. Kolodner (2006)
J. Cell Biol. 175, 729-741
   Abstract »    Full Text »    PDF »
From the Cover: UV irradiation induces a postreplication DNA damage checkpoint.
A. J. Callegari and T. J. Kelly (2006)
PNAS 103, 15877-15882
   Abstract »    Full Text »    PDF »
Regulation of DNA Replication Machinery by Mrc1 in Fission Yeast.
N. Nitani, K.-i. Nakamura, C. Nakagawa, H. Masukata, and T. Nakagawa (2006)
Genetics 174, 155-165
   Abstract »    Full Text »    PDF »
Rad4TopBP1, a Scaffold Protein, Plays Separate Roles in DNA Damage and Replication Checkpoints and DNA Replication.
L. Taricani and T. S.F. Wang (2006)
Mol. Biol. Cell 17, 3456-3468
   Abstract »    Full Text »    PDF »
Two-stage mechanism for activation of the DNA replication checkpoint kinase Cds1 in fission yeast.
Y.-j. Xu, M. Davenport, and T. J. Kelly (2006)
Genes & Dev. 20, 990-1003
   Abstract »    Full Text »    PDF »
Rhp51-Dependent Recombination Intermediates That Do Not Generate Checkpoint Signal Are Accumulated in Schizosaccharomyces pombe rad60 and smc5/6 Mutants after Release from Replication Arrest.
I. Miyabe, T. Morishita, T. Hishida, S. Yonei, and H. Shinagawa (2006)
Mol. Cell. Biol. 26, 343-353
   Abstract »    Full Text »    PDF »
Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.
M. A. Marchetti, M. Weinberger, Y. Murakami, W. C. Burhans, and J. A. Huberman (2006)
J. Cell Sci. 119, 124-131
   Abstract »    Full Text »    PDF »
Protein Phosphatase 5 Is Required for ATR-Mediated Checkpoint Activation.
J. Zhang, S. Bao, R. Furumai, K. S. Kucera, A. Ali, N. M. Dean, and X.-F. Wang (2005)
Mol. Cell. Biol. 25, 9910-9919
   Abstract »    Full Text »    PDF »
The Fission Yeast Crb2/Chk1 Pathway Coordinates the DNA Damage and Spindle Checkpoint in Response to Replication Stress Induced by Topoisomerase I Inhibitor.
A. Collura, J. Blaisonneau, G. Baldacci, and S. Francesconi (2005)
Mol. Cell. Biol. 25, 7889-7899
   Abstract »    Full Text »    PDF »
Regulation of mitosis in response to damaged or incompletely replicated DNA require different levels of Grapes (Drosophila Chk1).
A. Purdy, L. Uyetake, M. G. Cordeiro, and T. T. Su (2005)
J. Cell Sci. 118, 3305-3315
   Abstract »    Full Text »    PDF »
Grp/DChk1 is required for G2-M checkpoint activation in Drosophila S2 cells, whereas Dmnk/DChk2 is dispensable.
H. I. de Vries, L. Uyetake, W. Lemstra, J. F. Brunsting, T. T. Su, H. H. Kampinga, and O. C. M. Sibon (2005)
J. Cell Sci. 118, 1833-1842
   Abstract »    Full Text »    PDF »
Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress.
M. Kai, M. N. Boddy, P. Russell, and T. S.-F. Wang (2005)
Genes & Dev. 19, 919-932
   Abstract »    Full Text »    PDF »
Meiotic S-Phase Damage Activates Recombination without Checkpoint Arrest.
D. G. Pankratz and S. L. Forsburg (2005)
Mol. Biol. Cell 16, 1651-1660
   Abstract »    Full Text »    PDF »
Methylator-induced, Mismatch Repair-dependent G2 Arrest Is Activated through Chk1 and Chk2.
A. W. Adamson, D. I. Beardsley, W.-J. Kim, Y. Gao, R. Baskaran, and K. D. Brown (2005)
Mol. Biol. Cell 16, 1513-1526
   Abstract »    Full Text »    PDF »
Chk1-Dependent S-M Checkpoint Delay in Vertebrate Cells Is Linked to Maintenance of Viable Replication Structures.
G. Zachos, M. D. Rainey, and D. A. F. Gillespie (2005)
Mol. Cell. Biol. 25, 563-574
   Abstract »    Full Text »    PDF »
Mcl1p Is a Polymerase {alpha} Replication Accessory Factor Important for S-Phase DNA Damage Survival.
D. R. Williams and J. R. McIntosh (2005)
Eukaryot. Cell 4, 166-177
   Abstract »    Full Text »    PDF »
DNA Binding Domain in the Replication Checkpoint Protein Mrc1 of Schizosaccharomyces pombe.
H. Zhao and P. Russell (2004)
J. Biol. Chem. 279, 53023-53027
   Abstract »    Full Text »    PDF »
DNA Replication Checkpoint Control Mediated by the Spindle Checkpoint Protein Mad2p in Fission Yeast.
I. Sugimoto, H. Murakami, Y. Tonami, A. Moriyama, and M. Nakanishi (2004)
J. Biol. Chem. 279, 47372-47378
   Abstract »    Full Text »    PDF »
Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast.
E. Noguchi, C. Noguchi, W. H. McDonald, J. R. Yates III, and P. Russell (2004)
Mol. Cell. Biol. 24, 8342-8355
   Abstract »    Full Text »    PDF »
A Role for Saccharomyces cerevisiae Chk1p in the Response to Replication Blocks.
K. L. Schollaert, J. M. Poisson, J. S. Searle, J. A. Schwanekamp, C. R. Tomlinson, and Y. Sanchez (2004)
Mol. Biol. Cell 15, 4051-4063
   Abstract »    Full Text »    PDF »
Cds1 Phosphorylation by Rad3-Rad26 Kinase Is Mediated by Forkhead-associated Domain Interaction with Mrc1.
K. Tanaka and P. Russell (2004)
J. Biol. Chem. 279, 32079-32086
   Abstract »    Full Text »    PDF »
DNA damage responses triggered by a highly cytotoxic monofunctional DNA alkylator, hedamycin, a pluramycin antitumor antibiotic.
L. C. Tu, T. Melendy, and T. A. Beerman (2004)
Mol. Cancer Ther. 3, 577-586
   Abstract »    Full Text »    PDF »
M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCF{beta}-TrCP.
N. Watanabe, H. Arai, Y. Nishihara, M. Taniguchi, N. Watanabe, T. Hunter, and H. Osada (2004)
PNAS 101, 4419-4424
   Abstract »    Full Text »    PDF »
Wee1-dependent mechanisms required for coordination of cell growth and cell division.
D. R. Kellogg (2003)
J. Cell Sci. 116, 4883-4890
   Abstract »    Full Text »    PDF »
Stabilization of stalled DNA replication forks by the BRCA2 breast cancer susceptibility protein.
M. Lomonosov, S. Anand, M. Sangrithi, R. Davies, and A. R. Venkitaraman (2003)
Genes & Dev. 17, 3017-3022
   Abstract »    Full Text »    PDF »
Induction of S. cerevisiae Filamentous Differentiation by Slowed DNA Synthesis Involves Mec1, Rad53 and Swe1 Checkpoint Proteins.
Y. W. Jiang and C. M. Kang (2003)
Mol. Biol. Cell 14, 5116-5124
   Abstract »    Full Text »    PDF »
Swi1 Prevents Replication Fork Collapse and Controls Checkpoint Kinase Cds1.
E. Noguchi, C. Noguchi, L.-L. Du, and P. Russell (2003)
Mol. Cell. Biol. 23, 7861-7874
   Abstract »    Full Text »    PDF »
Schizosaccharomyces pombe Ddb1 Is functionally Linked to the Replication Checkpoint Pathway.
T. Bondar, E. V. Mirkin, D. S. Ucker, W. E. Walden, S. M. Mirkin, and P. Raychaudhuri (2003)
J. Biol. Chem. 278, 37006-37014
   Abstract »    Full Text »    PDF »
Molecular Characterization of the Schizosaccharomyces pombe nbs1+ Gene Involved in DNA Repair and Telomere Maintenance.
M. Ueno, T. Nakazaki, Y. Akamatsu, K. Watanabe, K. Tomita, H. D. Lindsay, H. Shinagawa, and H. Iwasaki (2003)
Mol. Cell. Biol. 23, 6553-6563
   Abstract »    Full Text »    PDF »
The Fission Yeast Rad32 (Mre11)-Rad50-Nbs1 Complex Is Required for the S-Phase DNA Damage Checkpoint.
C. Chahwan, T. M. Nakamura, S. Sivakumar, P. Russell, and N. Rhind (2003)
Mol. Cell. Biol. 23, 6564-6573
   Abstract »    Full Text »    PDF »
Replication Checkpoint Kinase Cds1 Regulates Recombinational Repair Protein Rad60.
M. N. Boddy, P. Shanahan, W. H. McDonald, A. Lopez-Girona, E. Noguchi, J. R. Yates III, and P. Russell (2003)
Mol. Cell. Biol. 23, 5939-5946
   Abstract »    Full Text »    PDF »
Genetic Models in Applied Physiology: Invited Review: Effect of oxygen deprivation on cell cycle activity: a profile of delay and arrest.
R. M. Douglas and G. G. Haddad (2003)
J Appl Physiol 94, 2068-2083
   Abstract »    Full Text »    PDF »
Essential and dispensable roles of ATR in cell cycle arrest and genome maintenance.
E. J. Brown and D. Baltimore (2003)
Genes & Dev. 17, 615-628
   Abstract »    Full Text »    PDF »
Regulation of meiotic progression by the meiosis-specific checkpoint kinase Mek1 in fission yeast.
L. Perez-Hidalgo, S. Moreno, and P. A. San-Segundo (2003)
J. Cell Sci. 116, 259-271
   Abstract »    Full Text »    PDF »
Disruption of the checkpoint kinase 1/cell division cycle 25A pathway abrogates ionizing radiation-induced S and G2 checkpoints.
H. Zhao, J. L. Watkins, and H. Piwnica-Worms (2002)
PNAS 99, 14795-14800
   Abstract »    Full Text »    PDF »
Characterization of a Schizosaccharomyces pombe Strain Deleted for a Sequence Homologue of the Human Damaged DNA Binding 1 (DDB1) Gene.
F. Zolezzi, J. Fuss, S. Uzawa, and S. Linn (2002)
J. Biol. Chem. 277, 41183-41191
   Abstract »    Full Text »    PDF »
mcl1+, the Schizosaccharomyces pombe Homologue of CTF4, Is Important for Chromosome Replication, Cohesion, and Segregation.
D. R. Williams and J. R. McIntosh (2002)
Eukaryot. Cell 1, 758-773
   Abstract »    Full Text »    PDF »
Telomere Binding of Checkpoint Sensor and DNA Repair Proteins Contributes to Maintenance of Functional Fission Yeast Telomeres.
T. M. Nakamura, B. A. Moser, and P. Russell (2002)
Genetics 161, 1437-1452
   Abstract »    Full Text »    PDF »
14-3-3 Binding Regulates Catalytic Activity of Human Wee1 Kinase.
C. J. Rothblum-Oviatt, C. E. Ryan, and H. Piwnica-Worms (2001)
Cell Growth Differ. 12, 581-589
   Abstract »    Full Text »    PDF »
Involvement of the Interaction between p21 and Proliferating Cell Nuclear Antigen for the Maintenance of G2/M Arrest after DNA Damage.
T. Ando, T. Kawabe, H. Ohara, B. Ducommun, M. Itoh, and T. Okamoto (2001)
J. Biol. Chem. 276, 42971-42977
   Abstract »    Full Text »    PDF »
Characterization of Schizosaccharomyces pombe mcm7+ and cdc23+ (MCM10) and Interactions With Replication Checkpoints.
D. T. Liang and S. L. Forsburg (2001)
Genetics 159, 471-486
   Abstract »    Full Text »    PDF »
Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing.
C. Feijoo, C. Hall-Jackson, R. Wu, D. Jenkins, J. Leitch, D. M. Gilbert, and C. Smythe (2001)
J. Cell Biol. 154, 913-924
   Abstract »    Full Text »    PDF »
Schizosaccharomyces pombe Cells Lacking the Amino-Terminal Catalytic Domains of DNA Polymerase Epsilon Are Viable but Require the DNA Damage Checkpoint Control.
W. Feng and G. D'Urso (2001)
Mol. Cell. Biol. 21, 4495-4504
   Abstract »    Full Text »    PDF »
ATR-Mediated Checkpoint Pathways Regulate Phosphorylation and Activation of Human Chk1.
H. Zhao and H. Piwnica-Worms (2001)
Mol. Cell. Biol. 21, 4129-4139
   Abstract »    Full Text »    PDF »
Fission Yeast Rad17 Associates with Chromatin in Response to Aberrant Genomic Structures.
M. Kai, H. Tanaka, and T. S.-F. Wang (2001)
Mol. Cell. Biol. 21, 3289-3301
   Abstract »    Full Text »
Threonine-11, Phosphorylated by Rad3 and ATM In Vitro, Is Required for Activation of Fission Yeast Checkpoint Kinase Cds1.
K. Tanaka, M. N. Boddy, X.-B. Chen, C. H. McGowan, and P. Russell (2001)
Mol. Cell. Biol. 21, 3398-3404
   Abstract »    Full Text »
Regulation of Initiation of S Phase, Replication Checkpoint Signaling, and Maintenance of Mitotic Chromosome Structures during S Phase by Hsk1 Kinase in the Fission Yeast.
T. Takeda, K. Ogino, K. Tatebayashi, H. Ikeda, K.-i. Arai, and H. Masai (2001)
Mol. Biol. Cell 12, 1257-1274
   Abstract »    Full Text »
Roles of the Mitotic Inhibitors Wee1 and Mik1 in the G2 DNA Damage and Replication Checkpoints.
N. Rhind and P. Russell (2001)
Mol. Cell. Biol. 21, 1499-1508
   Abstract »    Full Text »
Positive Regulation of Wee1 by Chk1 and 14-3-3 Proteins.
J. Lee, A. Kumagai, and W. G. Dunphy (2001)
Mol. Biol. Cell 12, 551-563
   Abstract »    Full Text »
S-Phase Arrest by Nucleoside Analogues and Abrogation of Survival without Cell Cycle Progression by 7-Hydroxystaurosporine.
Z. Shi, A. Azuma, D. Sampath, Y.-X. Li, P. Huang, and W. Plunkett (2001)
Cancer Res. 61, 1065-1072
   Abstract »    Full Text »
Role of Fission Yeast Primase Catalytic Subunit in the Replication Checkpoint.
D. J. F. Griffiths, V. F. Liu, P. Nurse, and T. S.-F. Wang (2001)
Mol. Biol. Cell 12, 115-128
   Abstract »    Full Text »
Damage Tolerance Protein Mus81 Associates with the FHA1 Domain of Checkpoint Kinase Cds1.
M. N. Boddy, A. Lopez-Girona, P. Shanahan, H. Interthal, W.-D. Heyer, and P. Russell (2000)
Mol. Cell. Biol. 20, 8758-8766
   Abstract »    Full Text »
Analysis of Fission Yeast Primase Defines the Checkpoint Responses to Aberrant S Phase Initiation.
S. Tan and T. S.-F. Wang (2000)
Mol. Cell. Biol. 20, 7853-7866
   Abstract »    Full Text »
Bypass of a Meiotic Checkpoint by Overproduction of Meiotic Chromosomal Proteins.
J. M. Bailis, A. V. Smith, and G. S. Roeder (2000)
Mol. Cell. Biol. 20, 4838-4848
   Abstract »    Full Text »
Mechanism of Caffeine-Induced Checkpoint Override in Fission Yeast.
B. A. Moser, J.-M. Brondello, B. Baber-Furnari, and P. Russell (2000)
Mol. Cell. Biol. 20, 4288-4294
   Abstract »    Full Text »
Chk1 is an essential kinase that is regulated by Atr and required for the G2/M DNA damage checkpoint.
Q. Liu, S. Guntuku, X.-S. Cui, S. Matsuoka, D. Cortez, K. Tamai, G. Luo, S. Carattini-Rivera, F. DeMayo, A. Bradley, et al. (2000)
Genes & Dev. 14, 1448-1459
   Abstract »    Full Text »
Slm9, a Novel Nuclear Protein Involved in Mitotic Control in Fission Yeast.
J. Kanoh and P. Russell (2000)
Genetics 155, 623-631
   Abstract »    Full Text »
Cid1, a Fission Yeast Protein Required for S-M Checkpoint Control when DNA Polymerase delta or varepsilon Is Inactivated.
S.-W. Wang, T. Toda, R. MacCallum, A. L. Harris, and C. Norbury (2000)
Mol. Cell. Biol. 20, 3234-3244
   Abstract »    Full Text »
Response of Xenopus Cds1 in Cell-free Extracts to DNA Templates with Double-stranded Ends.
Z. Guo and W. G. Dunphy (2000)
Mol. Biol. Cell 11, 1535-1546
   Abstract »    Full Text »
Binding of 14-3-3{beta} to the Carboxyl Terminus of Wee1 Increases Wee1 Stability, Kinase Activity, and G2-M Cell Population.
Y. Wang, C. Jacobs, K. E. Hook, H. Duan, R. N. Booher, and Y. Sun (2000)
Cell Growth Differ. 11, 211-219
   Abstract »    Full Text »
Genetic Studies with the Fission Yeast Schizosaccharomyces pombe Suggest Involvement of Wee1, Ppa2, and Rad24 in Induction of Cell Cycle Arrest by Human Immunodeficiency Virus Type 1 Vpr.
M. Masuda, Y. Nagai, N. Oshima, K. Tanaka, H. Murakami, H. Igarashi, and H. Okayama (2000)
J. Virol. 74, 2636-2646
   Abstract »    Full Text »
Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis.
P. U. Christensen, N. J. Bentley, R. G. Martinho, O. Nielsen, and A. M. Carr (2000)
PNAS 97, 2579-2584
   Abstract »    Full Text »    PDF »
The Chk1 Protein Kinase and the Cdc25C Regulatory Pathways Are Targets of the Anticancer Agent UCN-01.
P. R. Graves, L. Yu, J. K. Schwarz, J. Gales, E. A. Sausville, P. M. O'Connor, and H. Piwnica-Worms (2000)
J. Biol. Chem. 275, 5600-5605
   Abstract »    Full Text »    PDF »
Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9.
T. Caspari, M. Dahlen, G. Kanter-Smoler, H. D. Lindsay, K. Hofmann, K. Papadimitriou, P. Sunnerhagen, and A. M. Carr (2000)
Mol. Cell. Biol. 20, 1254-1262
   Abstract »    Full Text »
An Indolocarbazole Inhibitor of Human Checkpoint Kinase (Chk1) Abrogates Cell Cycle Arrest Caused by DNA Damage.
J. R. Jackson, A. Gilmartin, C. Imburgia, J. D. Winkler, L. A. Marshall, and A. Roshak (2000)
Cancer Res. 60, 566-572
   Abstract »    Full Text »
Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53.
N. H. Chehab, A. Malikzay, M. Appel, and T. D. Halazonetis (2000)
Genes & Dev. 14, 278-288
   Abstract »    Full Text »
The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast.
A Borgne and P Nurse (2000)
J. Cell Sci. 113, 4341-4350
   Abstract »    PDF »
Chk1 and Cds1: linchpins of the DNA damage and replication checkpoint pathways.
N Rhind and P Russell (2000)
J. Cell Sci. 113, 3889-3896
   Abstract »    PDF »
A S/M DNA replication checkpoint prevents nuclear and cytoplasmic events of cell division including centrosomal axis alignment and inhibits activation of cyclin-dependent kinase-like proteins in fucoid zygotes.
F Corellou, S. Bisgrove, D. Kropf, L Meijer, B Kloareg, and F. Bouget (2000)
Development 127, 1651-1660
   Abstract »    PDF »
A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints.
D Griffiths, M Uchiyama, P Nurse, and T. Wang (2000)
J. Cell Sci. 113, 1075-1088
   Abstract »    PDF »
Establishment of and Recovery from Damage Checkpoint Requires Sequential Interactions of Crb2 with Protein Kinases Rad3, Chk1, and Cdc2.
F. ESASHI, S. MOCHIDA, T. MATSUSAKA, T. OBARA, A. OGAWA, K. TAMAI, and M. YANAGIDA (2000)
Cold Spring Harb Symp Quant Biol 65, 443-450
   Abstract »    PDF »
The DNA Damage Checkpoint and Human Cancer.
L.B. SCHULTZ, N.H. CHEHAB, A. MALIKZAY, R.A. DITULLIO, E.S. STAVRIDI, and T.D. HALAZONETIS (2000)
Cold Spring Harb Symp Quant Biol 65, 489-498
   Abstract »    PDF »
The Function of BRCA1 in DNA Damage Response.
J.-S. LEE, K. COLLINS, A. BROWN, C.-H. LEE, and J.H. CHUNG (2000)
Cold Spring Harb Symp Quant Biol 65, 547-552
   Abstract »    PDF »
Regulation of Mitotic Inhibitor Mik1 Helps to Enforce the DNA Damage Checkpoint.
B. A. Baber-Furnari, N. Rhind, M. N. Boddy, P. Shanahan, A. Lopez-Girona, and P. Russell (2000)
Mol. Biol. Cell 11, 1-11
   Abstract »    Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)