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Science 8 May 1998:
Vol. 280. no. 5365, pp. 905 - 908
DOI: 10.1126/science.280.5365.905
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Reports

Thymocyte Development in the Absence of Pre-T Cell Receptor Extracellular Immunoglobulin Domains

Bryan A. Irving, Frederick W. Alt, Nigel Killeen *

Immature thymocytes express a pre-T cell receptor (pre-TCR) composed of the TCRbeta chain paired with pre-Talpha . Signals from this receptor are essential for passage of thymocytes through a key developmental checkpoint in the thymus. These signals were efficiently delivered in vivo by a truncated form of the murine pre-TCR that lacked all of its extracellular immunoglobulin domains. De novo expression of the truncated pre-TCR or an intact alpha beta TCR was sufficient to activate characteristic TCR signaling pathways in a T cell line. These findings support the view that recognition of an extracellular ligand is not required for pre-TCR function.

B. A. Irving and N. Killeen, Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0414, USA.
F. W. Alt, Howard Hughes Medical Institute and Department of Genetics and Pediatrics, Children's Hospital and Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA.
*   To whom correspondence should be addressed. E-mail: nigel{at}itsa.ucsf.edu

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