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Science 24 April 1998:
Vol. 280. no. 5363, pp. 605 - 608
DOI: 10.1126/science.280.5363.605
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Reports

A Distinctive Class of Integron in the Vibrio cholerae Genome

Didier Mazel, * Broderick Dychinco, Vera A. Webb, Julian Davies dagger

The ability of bacteria to acquire and disseminate heterologous genes has been a major factor in the development of multiple drug resistance. A gene, intI4, was identified that encodes a previously unknown integrase that is associated with a "gene-VCR" organization (VCRs are Vibrio cholerae repeated sequences), similar to that of the well-characterized antibiotic resistance integrons. The similarity was confirmed by IntI1-mediated recombination of a gene-VCR cassette into a class 1 integron. VCR cassettes are found in a number of Vibrio species including a strain of V. metschnikovii isolated in 1888, suggesting that this mechanism of heterologous gene acquisition predated the antibiotic era.

Department of Microbiology and Immunology, University of British Columbia, 6174 University Boulevard, Vancouver, B.C., V6T 1Z3, Canada.
*   Present address: Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris Cedex 15, France.

dagger    To whom correspondence should be addressed.

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