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Science 24 April 1998:
Vol. 280. no. 5363, pp. 602 - 605
DOI: 10.1126/science.280.5363.602
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Reports

Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System

Tomoko Kubori, * Yukiyasu Matsushima, Dai Nakamura, Jaimol Uralil, Maria Lara-Tejero, Anand Sukhan, Jorge E. Galán, dagger Shin-Ichi Aizawa

The type III secretion system of Salmonella typhimurium directs the translocation of proteins into host cells. Evolutionarily related to the flagellar assembly machinery, this system is also present in other pathogenic bacteria, but its organization is unknown. Electron microscopy revealed supramolecular structures spanning the inner and outer membranes of flagellated and nonflagellated strains; such structures were not detected in strains carrying null mutations in components of the type III apparatus. Isolated structures were found to contain at least three proteins of this secretion system. Thus, the type III apparatus of S. typhimurium, and presumably other bacteria, exists as a supramolecular structure in the bacterial envelope.

T. Kubori, Y. Matsushima, D. Nakamura, S.-I. Aizawa, Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320, Japan.
J. Uralil, M. Lara-Tejero, A. Sukhan, J. E. Galán, Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York, Stony Brook, NY 11794, USA.
*   Present address: Structural Biology Center, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.

dagger    To whom correspondence should be addressed. E-mail: galan{at}asterix.bio.sunysb.edu

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D. M. Anderson, K. S. Ramamurthi, C. Tam, and O. Schneewind (2002)
J. Bacteriol. 184, 1287-1295
   Abstract »    Full Text »    PDF »
YplA Is Exported by the Ysc, Ysa, and Flagellar Type III Secretion Systems of Yersinia enterocolitica.
B. M. Young and G. M. Young (2002)
J. Bacteriol. 184, 1324-1334
   Abstract »    Full Text »    PDF »
Genes Required for Plasmid R64 Thin-Pilus Biogenesis: Identification and Localization of Products of the pilK, pilM, pilO, pilP, pilR, and pilT Genes.
D. Sakai and T. Komano (2002)
J. Bacteriol. 184, 444-451
   Abstract »    Full Text »    PDF »
MxiM and MxiJ, Base Elements of the Mxi-Spa Type III Secretion System of Shigella, Interact with and Stabilize the MxiD Secretin in the Cell Envelope.
R. Schuch and A. T. Maurelli (2001)
J. Bacteriol. 183, 6991-6998
   Abstract »    Full Text »    PDF »
The HilA Box and Sequences outside It Determine the Magnitude of HilA-Dependent Activation of PprgH from Salmonella Pathogenicity Island 1.
C. P. Lostroh and C. A. Lee (2001)
J. Bacteriol. 183, 4876-4885
   Abstract »    Full Text »    PDF »
Protein secretion and the pathogenesis of bacterial infections.
V. T. Lee and O. Schneewind (2001)
Genes & Dev. 15, 1725-1752
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Spa33, a Cell Surface-Associated Subunit of the Mxi-Spa Type III Secretory Pathway of Shigella flexneri, Regulates Ipa Protein Traffic.
R. Schuch and A. T. Maurelli (2001)
Infect. Immun. 69, 2180-2189
   Abstract »    Full Text »    PDF »
Resistance of Pseudomonas aeruginosa Isolates to Hydrogel Contact Lens Disinfection Correlates with Cytotoxic Activity.
C. Lakkis and S. M. J. Fleiszig (2001)
J. Clin. Microbiol. 39, 1477-1486
   Abstract »    Full Text »    PDF »
Genetic Analysis of Assembly of the Salmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle Complex.
A. Sukhan, T. Kubori, J. Wilson, and J. E. Galan (2001)
J. Bacteriol. 183, 1159-1167
   Abstract »    Full Text »    PDF »
Subset of Hybrid Eukaryotic Proteins Is Exported by the Type I Secretion System of Erwinia chrysanthemi.
J. L. Palacios, I. Zaror, P. Martinez, F. Uribe, P. Opazo, T. Socias, M. Gidekel, and A. Venegas (2001)
J. Bacteriol. 183, 1346-1358
   Abstract »    Full Text »    PDF »
Type III secretion systems and pathogenicity islands.
C. WINSTANLEY and C. A. HART (2001)
J. Med. Microbiol. 50, 116-126
   Abstract »    Full Text »    PDF »
Coupling of Flagellar Gene Expression to Flagellar Assembly in Salmonella enterica Serovar Typhimurium and Escherichia coli.
G. S. Chilcott and K. T. Hughes (2000)
Microbiol. Mol. Biol. Rev. 64, 694-708
   Abstract »    Full Text »    PDF »
Regulation of Salmonella enterica Serovar Typhimurium Invasion Genes by csrA.
C. Altier, M. Suyemoto, and S. D. Lawhon (2000)
Infect. Immun. 68, 6790-6797
   Abstract »    Full Text »    PDF »
InvB Is a Type III Secretion Chaperone Specific for SspA.
P. A. Bronstein, E. A. Miao, and S. I. Miller (2000)
J. Bacteriol. 182, 6638-6644
   Abstract »    Full Text »    PDF »


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