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Science 24 April 1998:
Vol. 280. no. 5363, pp. 593 - 596
DOI: 10.1126/science.280.5363.593
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Reports

Formation of a Preinitiation Complex by S-phase Cyclin CDK-Dependent Loading of Cdc45p onto Chromatin

Lee Zou, * Bruce Stillman dagger

Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G1 and during the S phase of the cell cycle. Binding of Cdc45p to chromatin depends on Clb-Cdc28 kinase activity as well as functional Cdc6p and Mcm2p, which suggests that Cdc45p associates with the prereplication complex after activation of S-phase cyclin-dependent kinases (CDKs). As indicated by the timing and the CDK dependence, binding of Cdc45p to chromatin is crucial for commitment to initiation of DNA replication. During S phase, Cdc45p physically interacts with minichromosome maintenance (MCM) proteins on chromatin; however, dissociation of Cdc45p from chromatin is slower than that of MCMs, which indicates that the proteins are released by different mechanisms.

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
*   Is also a student in the Graduate Program in Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.

dagger    To whom correspondence should be addressed. E-mail: stillman{at}cshl.org

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