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Science 10 April 1998:
Vol. 280. no. 5361, pp. 298 - 301
DOI: 10.1126/science.280.5361.298
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Reports

Coupling Termination of Transcription to Messenger RNA Maturation in Yeast

Charles E. Birse, * Lionel Minvielle-Sebastia, Barbara A. Lee, Walter Keller, Nick J. Proudfoot dagger

The direct association between messenger RNA (mRNA) 3'-end processing and the termination of transcription was established for the CYC1 gene of Saccharomyces cerevisiae. The mutation of factors involved in the initial cleavage of the primary transcript at the poly(A) site (RNA14, RNA15, and PCF11) disrupted transcription termination at the 3' end of the CYC1 gene. In contrast, the mutation of factors involved in the subsequent polyadenylation step (PAP1, FIP1, and YTH1) had little effect. Thus, cleavage factors link transcription termination of RNA polymerase II with pre-mRNA 3'-end processing.

C. E. Birse, B. A. Lee, N. J. Proudfoot, Sir William Dunn School of Pathology, South Parks Road, University of Oxford, Oxford OX1 3RE, UK.
L. Minvielle-Sebastia and W. Keller, Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
*   Present address: Department of Biological Chemistry, 240 D Med Sci I, College of Medicine, University of California, Irvine, CA 92697, USA.

dagger    To whom correspondence should be addressed. E-mail: nicholas.proudfoot{at}path.ox.ac.uk

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Science. ISSN 0036-8075 (print), 1095-9203 (online)