Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Invitrogen

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 27 March 1998:
Vol. 279. no. 5359, pp. 2113 - 2115
DOI: 10.1126/science.279.5359.2113
Prev | Table of Contents | Next

Reports

Transcriptional and Posttranscriptional Plant Gene Silencing in Response to a Pathogen

Nadia S. Al-Kaff, * Simon N. Covey, Maria M. Kreike, Anthony M. Page, Rachel Pinder, Philip J. Dale

Plants are able to respond to pathogen attack to restrain development of a systemic infection. The response of Brassica napus (oilseed rape) to systemic infection with the DNA virus cauliflower mosaic virus was shown to result in enhancement and subsequent suppression of viral gene expression in parallel with changes in symptom expression. Transgenes with homology to viral sequences were also affected. This phenomenon, which was shown to be mediated by both transcriptional and posttranscriptional mechanisms, might be related to regulation of highly expressed genetic elements.

John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
*   To whom correspondence should be addressed. E-mail: alkaff{at}bbsrc.ac.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Effects of viral silencing suppressors on tobacco ringspot virus infection in two Nicotiana species.
S. A. Siddiqui, C. Sarmiento, M. Kiisma, S. Koivumaki, A. Lemmetty, E. Truve, and K. Lehto (2008)
J. Gen. Virol. 89, 1502-1508
   Abstract »    Full Text »    PDF »
Antiviral RNA Silencing Is Restricted to the Marginal Region of the Dark Green Tissue in the Mosaic Leaves of Tomato Mosaic Virus-Infected Tobacco Plants.
K. Hirai, K. Kubota, T. Mochizuki, S. Tsuda, and T. Meshi (2008)
J. Virol. 82, 3250-3260
   Abstract »    Full Text »    PDF »
Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing.
A. J. Love, J. Laird, J. Holt, A. J. Hamilton, A. Sadanandom, and J. J. Milner (2007)
J. Gen. Virol. 88, 3439-3444
   Abstract »    Full Text »    PDF »
Tomato Chlorotic Mottle Virus Is a Target of RNA Silencing but the Presence of Specific Short Interfering RNAs Does Not Guarantee Resistance in Transgenic Plants.
S. G. Ribeiro, H. Lohuis, R. Goldbach, and M. Prins (2007)
J. Virol. 81, 1563-1573
   Abstract »    Full Text »    PDF »
RNA silencing of host transcripts by cauliflower mosaic virus requires coordinated action of the four Arabidopsis Dicer-like proteins.
G. Moissiard and O. Voinnet (2006)
PNAS 103, 19593-19598
   Abstract »    Full Text »    PDF »
The Dominant Inhibitory Chalcone Synthase Allele C2-Idf (Inhibitor diffuse) From Zea mays (L.) Acts via an Endogenous RNA Silencing Mechanism.
C. B. Della Vedova, R. Lorbiecke, H. Kirsch, M. B. Schulte, K. Scheets, L. M. Borchert, B. E. Scheffler, U. Wienand, K. C. Cone, and J. A. Birchler (2005)
Genetics 170, 1989-2002
   Abstract »    Full Text »    PDF »
Aureusvirus P14 Is an Efficient RNA Silencing Suppressor That Binds Double-Stranded RNAs without Size Specificity.
Z. Merai, Z. Kerenyi, A. Molnar, E. Barta, A. Valoczi, G. Bisztray, Z. Havelda, J. Burgyan, and D. Silhavy (2005)
J. Virol. 79, 7217-7226
   Abstract »    Full Text »    PDF »
Argonaute protein PIWI controls mobilization of retrotransposons in the Drosophila male germline.
A. I. Kalmykova, M. S. Klenov, and V. A. Gvozdev (2005)
Nucleic Acids Res. 33, 2052-2059
   Abstract »    Full Text »    PDF »
La Crosse Virus Nonstructural Protein NSs Counteracts the Effects of Short Interfering RNA.
S. S. Soldan, M. L. Plassmeyer, M. K. Matukonis, and F. Gonzalez-Scarano (2005)
J. Virol. 79, 234-244
   Abstract »    Full Text »    PDF »
Short Interfering RNA Accumulation Correlates with Host Recovery in DNA Virus-Infected Hosts, and Gene Silencing Targets Specific Viral Sequences.
P. Chellappan, R. Vanitharani, and C. M. Fauquet (2004)
J. Virol. 78, 7465-7477
   Abstract »    Full Text »    PDF »
Viral Virulence Protein Suppresses RNA Silencing-Mediated Defense but Upregulates the Role of MicroRNA in Host Gene Expression.
J. Chen, W. X. Li, D. Xie, J. R. Peng, and S. W. Ding (2004)
PLANT CELL 16, 1302-1313
   Abstract »    Full Text »    PDF »
RNA Interference: Biology, Mechanism, and Applications.
N. Agrawal, P. V. N. Dasaradhi, A. Mohmmed, P. Malhotra, R. K. Bhatnagar, and S. K. Mukherjee (2003)
Microbiol. Mol. Biol. Rev. 67, 657-685
   Abstract »    Full Text »    PDF »
Nucleic Acid-Based Immune System: the Antiviral Potential of Mammalian RNA Silencing.
L. Gitlin and R. Andino (2003)
J. Virol. 77, 7159-7165
   Full Text »    PDF »
Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs.
G. Randall, A. Grakoui, and C. M. Rice (2003)
PNAS 100, 235-240
   Abstract »    Full Text »    PDF »
Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions.
E. Bucher, T. Sijen, P. de Haan, R. Goldbach, and M. Prins (2002)
J. Virol. 77, 1329-1336
   Abstract »    Full Text »    PDF »
Silencing of a viral RNA silencing suppressor in transgenic plants.
E. I. Savenkov and J. P. T. Valkonen (2002)
J. Gen. Virol. 83, 2325-2335
   Abstract »    Full Text »    PDF »
Spreading of RNA Targeting and DNA Methylation in RNA Silencing Requires Transcription of the Target Gene and a Putative RNA-Dependent RNA Polymerase.
F. E. Vaistij, L. Jones, and D. C. Baulcombe (2002)
PLANT CELL 14, 857-867
   Abstract »    Full Text »    PDF »
Transcriptional cosuppression of yeast Ty1 retrotransposons.
Y. W. Jiang (2002)
Genes & Dev. 16, 467-478
   Abstract »    Full Text »    PDF »
Post-transcriptional gene silencing in plants.
H. Vaucheret, C. Beclin, and M. Fagard (2001)
J. Cell Sci. 114, 3083-3091
   Abstract »    Full Text »    PDF »
Transient expression of a GUS reporter gene from cauliflower mosaic virus replacement vectors in the presence and absence of helper virus.
R. Viaplana, D. S. Turner, and S. N. Covey (2001)
J. Gen. Virol. 82, 59-65
   Abstract »    Full Text »
Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway.
C. Llave, K. D. Kasschau, and J. C. Carrington (2000)
PNAS
   Abstract »    Full Text »
Arabidopsis RTM2 Gene Is Necessary for Specific Restriction of Tobacco Etch Virus and Encodes an Unusual Small Heat Shock-like Protein.
S. A. Whitham, R. J. Anderberg, S. T. Chisholm, and J. C. Carrington (2000)
PLANT CELL 12, 569-582
   Abstract »    Full Text »
Silencing of Retrotransposons in Arabidopsis and Reactivation by the ddm1 Mutation.
H. Hirochika, H. Okamoto, and T. Kakutani (2000)
PLANT CELL 12, 357-369
   Abstract »    Full Text »
Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus.
S. T. Chisholm, S. K. Mahajan, S. A. Whitham, M. L. Yamamoto, and J. C. Carrington (2000)
PNAS 97, 489-494
   Abstract »    Full Text »    PDF »
Subcellular distribution analysis of the cucumber mosaic virus 2b protein.
C. N. Mayers, P. Palukaitis, and J. P. Carr (2000)
J. Gen. Virol. 81, 219-226
   Abstract »    Full Text »
Gene Silencing without DNA: RNA-Mediated Cross-Protection between Viruses.
F. G. Ratcliff, S. A. MacFarlane, and D. C. Baulcombe (1999)
PLANT CELL 11, 1207-1216
   Abstract »    Full Text »
Posttranscriptional Gene Silencing in Transgenic Sugarcane. Dissection of Homology-Dependent Virus Resistance in a Monocot That Has a Complex Polyploid Genome.
I. L. Ingelbrecht, J. E. Irvine, and T. E. Mirkov (1999)
Plant Physiology 119, 1187-1198
   Abstract »    Full Text »
Selectable viruses and altered susceptibility mutants in Arabidopsis thaliana.
S. A. Whitham, M. L. Yamamoto, and J. C. Carrington (1999)
PNAS 96, 772-777
   Abstract »    Full Text »    PDF »
Plant gene silencing regularized.
G. Bruening (1998)
PNAS 95, 13349-13351
   Full Text »    PDF »
A viral suppressor of gene silencing in plants.
R. Anandalakshmi, G. J. Pruss, X. Ge, R. Marathe, A. C. Mallory, T. H. Smith, and V. B. Vance (1998)
PNAS 95, 13079-13084
   Abstract »    Full Text »    PDF »
An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense.
Z. Xie, B. Fan, C. Chen, and Z. Chen (2001)
PNAS 98, 6516-6521
   Abstract »    Full Text »    PDF »
Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway.
C. Llave, K. D. Kasschau, and J. C. Carrington (2000)
PNAS 97, 13401-13406
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)