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Science 20 February 1998:
Vol. 279. no. 5354, pp. 1219 - 1222
DOI: 10.1126/science.279.5354.1219
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Reports

Identification of a Cullin Homology Region in a Subunit of the Anaphase-Promoting Complex

Hongtao Yu, Jan-Michael Peters, * Randall W. King, Andrew M. Page, Philip Hieter, Marc W. Kirschner dagger

The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratrico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G1 phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.

H. Yu, J.-M. Peters, R. W. King, M. W. Kirschner, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
A. M. Page and P. Hieter, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, BC, V6R2X8, Canada.
*   Present address: Research Institute for Molecular Pathology, Dr. Bohr-Gasse #7, A-1030 Vienna, Austria.

dagger    To whom correspondence should be addressed. E-mail: marc{at}hms.harvard.edu

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   Abstract »    Full Text »    PDF »
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S. L. Jaspersen, J. F. Charles, R. L. Tinker-Kulberg, and D. O. Morgan (1998)
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D. Patra and W. G. Dunphy (1998)
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T. Potuschak, S. Stary, P. Schlogelhofer, F. Becker, V. Nejinskaia, and A. Bachmair (1998)
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C. Lies, J Cheng, S. James, N. Morris, M. O'Connell, and P. Mirabito (1998)
J. Cell Sci. 111, 1453-1465
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Membrane Topology and Function of Der3/Hrd1p as a Ubiquitin-Protein Ligase (E3) Involved in Endoplasmic Reticulum Degradation.
P. M. Deak and D. H. Wolf (2001)
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Y.-Z. ZHANG, K. L. GOULD, R. L. DUNBRACK JR., H. CHENG, H. RODER, and E. A. GOLEMIS (1999)
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