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Science 20 February 1998:
Vol. 279. no. 5354, pp. 1216 - 1219
DOI: 10.1126/science.279.5354.1216
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Reports

Mass Spectrometric Analysis of the Anaphase-Promoting Complex from Yeast: Identification of a Subunit Related to Cullins

Wolfgang Zachariae, Andrej Shevchenko, Paul D. Andrews, Rafael Ciosk, Marta Galova, Michael J. R. Stark, Matthias Mann, * Kim Nasmyth *

Entry into anaphase and exit from mitosis depend on a ubiquitin-protein ligase complex called the anaphase-promoting complex (APC) or cyclosome. At least 12 different subunits were detected in the purified particle from budding yeast, including the previously identified proteins Apc1p, Cdc16p, Cdc23p, Cdc26p, and Cdc27p. Five additional subunits purified in low nanogram amounts were identified by tandem mass spectrometric sequencing. Apc2p, Apc5p, and the RING-finger protein Apc11p are conserved from yeast to humans. Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication.

W. Zachariae, R. Ciosk, M. Galova, K. Nasmyth, Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
A. Shevchenko and M. Mann, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69012 Heidelberg, Germany.
P. D. Andrews and M. J. R. Stark, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, UK.
*   To whom correspondence should be addressed.

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