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Science 13 February 1998:
Vol. 279. no. 5353, pp. 1045 - 1047
DOI: 10.1126/science.279.5353.1045
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Reports

Fission Yeast Slp1: An Effector of the Mad2-Dependent Spindle Checkpoint

Sang Hoon Kim, Diana P. Lin, Sanae Matsumoto, Ana Kitazono, Tomohiro Matsumoto *

Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.

Departments of Radiation Oncology and Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
*   To whom correspondence should be addressed.

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Fission Yeast Bub1 Is a Mitotic Centromere Protein Essential for the Spindle Checkpoint and the Preservation of Correct Ploidy through Mitosis.
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K. Nabeshima, T. Nakagawa, A. F. Straight, A. Murray, Y. Chikashige, Y. M. Yamashita, Y. Hiraoka, and M. Yanagida (1998)
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Mad2 transiently associates with an APC/p55Cdc complex during mitosis.
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   Abstract »    Full Text »    PDF »
Budding Yeast Cdc20: A Target of the Spindle Checkpoint.
L. H. Hwang, L. F. Lau, D. L. Smith, C. A. Mistrot, K. G. Hardwick, E. S. Hwang, A. Amon, and A. W. Murray (1998)
Science 279, 1041-1044
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Science. ISSN 0036-8075 (print), 1095-9203 (online)