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Science 13 February 1998:
Vol. 279. no. 5353, pp. 1041 - 1044
DOI: 10.1126/science.279.5353.1041
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Budding Yeast Cdc20: A Target of the Spindle Checkpoint

Lena H. Hwang, Lucius F. Lau, Dana L. Smith, Cathy A. Mistrot, Kevin G. Hardwick, * Ellen S. Hwang, Angelika Amon, Andrew W. Murray dagger

The spindle checkpoint regulates the cell division cycle by keeping cells with defective spindles from leaving mitosis. In the two-hybrid system, three proteins that are components of the checkpoint, Mad1, Mad2, and Mad3, were shown to interact with Cdc20, a protein required for exit from mitosis. Mad2 and Mad3 coprecipitated with Cdc20 at all stages of the cell cycle. The binding of Mad2 depended on Mad1 and that of Mad3 on Mad1 and Mad2. Overexpression of Cdc20 allowed cells with a depolymerized spindle or damaged DNA to leave mitosis but did not overcome the arrest caused by unreplicated DNA. Mutants in Cdc20 that were resistant to the spindle checkpoint no longer bound Mad proteins, suggesting that Cdc20 is the target of the spindle checkpoint.

L. H. Hwang, D. L. Smith, C. A. Mistrot, K. G. Hardwick, A. W. Murray, Departments of Physiology and Biochemistry, University of California at San Francisco, San Francisco, CA 94143-0444, USA.
L. F. Lau, E. S. Hwang, A. Amon, Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
*   Present address: Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR, UK.

dagger    To whom correspondence should be addressed. E-mail: amurray{at}socrates.ucsf.edu

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K. G. Hardwick, R. C. Johnston, D. L. Smith, and A. W. Murray (2000)
J. Cell Biol. 148, 871-882
   Abstract »    Full Text »    PDF »
High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.
H. Yamada, S Matsumoto, and T Matsumoto (2000)
J. Cell Sci. 113, 3989-3999
   Abstract »    PDF »
Mitotic Checkpoints, Genetic Instability, and Cancer.
M. DOBLES and P.K. SORGER (2000)
Cold Spring Harb Symp Quant Biol 65, 361-368
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)