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Science 6 February 1998:
Vol. 279. no. 5352, pp. 873 - 876
DOI: 10.1126/science.279.5352.873
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Reports

Conjugative Transfer by the Virulence System of Legionella pneumophila

Joseph. P. Vogel, Helene L. Andrews, Swee Kee Wong, Ralph R. Isberg *

Legionella pneumophila, the causative agent of Legionnaires' pneumonia, replicates within alveolar macrophages by preventing phagosome-lysosome fusion. Here, a large number of mutants called dot (defective for organelle trafficking) that were unable to replicate intracellularly because of an inability of the bacteria to alter the endocytic pathway of macrophages were isolated. The dot virulence genes encoded a large putative membrane complex that functioned as a secretion system that was able to transfer plasmid DNA from one cell to another.

J. P. Vogel, H. L. Andrews, S. K. Wong, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
R. R. Isberg, Department of Molecular Biology and Microbiology and Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, MA 02111, USA.
*   To whom correspondence should be addressed. E-mail: risberg{at}opal.tufts.edu

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J. Bacteriol. 184, 2123-2130
   Abstract »    Full Text »
Characterization and Transcriptional Analysis of Gene Clusters for a Type IV Secretion Machinery in Human Granulocytic and Monocytic Ehrlichiosis Agents.
N. Ohashi, N. Zhi, Q. Lin, and Y. Rikihisa (2002)
Infect. Immun. 70, 2128-2138
   Abstract »    Full Text »    PDF »
Genetic and Phenotypic Differences between Legionella pneumophila Strains.
M. M. Samrakandi, S. L. G. Cirillo, D. A. Ridenour, L. E. Bermudez, and J. D. Cirillo (2002)
J. Clin. Microbiol. 40, 1352-1362
   Abstract »    Full Text »    PDF »
How the parasitic bacterium Legionella pneumophila modifies its phagosome and transforms it into rough ER: implications for conversion of plasma membrane to the ER membrane.
L. G. Tilney, O. S. Harb, P. S. Connelly, C. G. Robinson, and C. R. Roy (2002)
J. Cell Sci. 114, 4637-4650
   Abstract »    Full Text »    PDF »
The Dot/Icm Type IV Secretion System of Legionella pneumophila Is Essential for the Induction of Apoptosis in Human Macrophages.
S. D. Zink, L. Pedersen, N. P. Cianciotto, and Y. Abu Kwaik (2002)
Infect. Immun. 70, 1657-1663
   Abstract »    Full Text »    PDF »
The Brucella suis virB operon is induced intracellularly in macrophages.
M. L. Boschiroli, S. Ouahrani-Bettache, V. Foulongne, S. Michaux-Charachon, G. Bourg, A. Allardet-Servent, C. Cazevieille, J. P. Liautard, M. Ramuz, and D. O'Callaghan (2002)
PNAS 99, 1544-1549
   Abstract »    Full Text »    PDF »
A Bacterial Guanine Nucleotide Exchange Factor Activates ARF on Legionella Phagosomes.
H. Nagai, J. C. Kagan, X. Zhu, R. A. Kahn, and C. R. Roy (2002)
Science 295, 679-682
   Abstract »    Full Text »    PDF »
icmT Is Essential for Pore Formation-Mediated Egress of Legionella pneumophila from Mammalian and Protozoan Cells.
M. Molmeret, O. A. T. Alli, S. Zink, A. Flieger, N. P. Cianciotto, and Y. A. Kwaik (2002)
Infect. Immun. 70, 69-78
   Abstract »    Full Text »    PDF »
Characterization of a Legionella pneumophila relA Insertion Mutant and Roles of RelA and RpoS in Virulence Gene Expression.
T. Zusman, O. Gal-Mor, and G. Segal (2002)
J. Bacteriol. 184, 67-75
   Abstract »    Full Text »    PDF »


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