Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 30 January 1998:
Vol. 279. no. 5351, pp. 696 - 700
DOI: 10.1126/science.279.5351.696
Prev | Table of Contents | Next

Reports

Role of Vesicle-Associated Syntaxin 5 in the Assembly of Pre-Golgi Intermediates

Tony Rowe, * Christiane Dascher, * Sergei Bannykh, Helen Plutner, William E. Balch dagger

Syntaxins are thought to function during vesicular transport as receptors on the target membrane and to contribute to the specificity of membrane docking and fusion by interacting with vesicle-associated receptors. Here, syntaxin 5 (Syn5) was shown to be an integral component of endoplasmic reticulum-derived transport vesicles. This pool, but not the target, Golgi-associated Syn5 pool, was essential for the assembly of vesicular-tubular pre-Golgi intermediates and the delivery of cargo to the Golgi. The requirement for vesicle-associated Syn5 in transport suggests a reevaluation of the basis for operation of the early secretory pathway.

Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: webalch{at}scripps.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Syntaxin 5 regulates the endoplasmic reticulum channel-release properties of polycystin-2.
L. Geng, W. Boehmerle, Y. Maeda, D. Y. Okuhara, X. Tian, Z. Yu, C.-u. Choe, G. I. Anyatonwu, B. E. Ehrlich, and S. Somlo (2008)
PNAS 105, 15920-15925
   Abstract »    Full Text »    PDF »
Erv26p Directs Pro-Alkaline Phosphatase into Endoplasmic Reticulum-derived Coat Protein Complex II Transport Vesicles.
C. A. Bue, C. M. Bentivoglio, and C. Barlowe (2006)
Mol. Biol. Cell 17, 4780-4789
   Abstract »    Full Text »    PDF »
Structure-based Functional Analysis Reveals a Role for the SM Protein Sly1p in Retrograde Transport to the Endoplasmic Reticulum.
Y. Li, D. Gallwitz, and R. Peng (2005)
Mol. Biol. Cell 16, 3951-3962
   Abstract »    Full Text »    PDF »
Reconstitution of COPII vesicle fusion to generate a pre-Golgi intermediate compartment.
D. Xu and J. C. Hay (2004)
J. Cell Biol. 167, 997-1003
   Abstract »    Full Text »    PDF »
Proteomics of Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC) Membranes from Brefeldin A-treated HepG2 Cells Identifies ERGIC-32, a New Cycling Protein That Interacts with Human Erv46.
L. Breuza, R. Halbeisen, P. Jeno, S. Otte, C. Barlowe, W. Hong, and H.-P. Hauri (2004)
J. Biol. Chem. 279, 47242-47253
   Abstract »    Full Text »    PDF »
Participation of the Syntaxin 5/Ykt6/GS28/GS15 SNARE Complex in Transport from the Early/Recycling Endosome to the Trans-Golgi Network.
G. Tai, L. Lu, T. L. Wang, B. L. Tang, B. Goud, L. Johannes, and W. Hong (2004)
Mol. Biol. Cell 15, 4011-4022
   Abstract »    Full Text »    PDF »
Sec22p Export from the Endoplasmic Reticulum Is Independent of SNARE Pairing.
Y. Liu, J. J. Flanagan, and C. Barlowe (2004)
J. Biol. Chem. 279, 27225-27232
   Abstract »    Full Text »    PDF »
Dynamics and inheritance of the endoplasmic reticulum.
Y. Du, S. Ferro-Novick, and P. Novick (2004)
J. Cell Sci. 117, 2871-2878
   Abstract »    Full Text »    PDF »
Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack.
A. Volchuk, M. Ravazzola, A. Perrelet, W. S. Eng, M. Di Liberto, O. Varlamov, M. Fukasawa, T. Engel, T. H. Sollner, J. E. Rothman, et al. (2004)
Mol. Biol. Cell 15, 1506-1518
   Abstract »    Full Text »    PDF »
p97, a protein coping with multiple identities.
P. G. Woodman (2003)
J. Cell Sci. 116, 4283-4290
   Abstract »    Full Text »    PDF »
Evidence for endocytosis of ROMK potassium channel via clathrin-coated vesicles.
W.-Z. Zeng, V. Babich, B. Ortega, R. Quigley, S. J. White, P. A. Welling, and C.-L. Huang (2002)
Am J Physiol Renal Physiol 283, F630-F639
   Abstract »    Full Text »    PDF »
GS15 Forms a SNARE Complex with Syntaxin 5, GS28, and Ykt6 and Is Implicated in Traffic in the Early Cisternae of the Golgi Apparatus.
Y. Xu, S. Martin, D. E. James, and W. Hong (2002)
Mol. Biol. Cell 13, 3493-3507
   Abstract »    Full Text »    PDF »
Analysis of Sec22p in Endoplasmic Reticulum/Golgi Transport Reveals Cellular Redundancy in SNARE Protein Function.
Y. Liu and C. Barlowe (2002)
Mol. Biol. Cell 13, 3314-3324
   Abstract »    Full Text »    PDF »
Non-conventional Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator through the Early Secretory Pathway.
J.-S. Yoo, B. D. Moyer, S. Bannykh, H.-M. Yoo, J. R. Riordan, and W. E. Balch (2002)
J. Biol. Chem. 277, 11401-11409
   Abstract »    Full Text »    PDF »
Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform.
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes (2002)
J. Cell Biol. 156, 653-664
   Abstract »    Full Text »    PDF »
Syntaxin 3 is required for cAMP-induced acid secretion: streptolysin O-permeabilized gastric gland model.
D. A. Ammar, R. Zhou, J. G. Forte, and X. Yao (2002)
Am J Physiol Gastrointest Liver Physiol 282, G23-G33
   Abstract »    Full Text »    PDF »
The Organizing Potential of Sphingolipids in Intracellular Membrane Transport.
J. C. M. Holthuis, T. Pomorski, R. J. Raggers, H. Sprong, and G. Van Meer (2001)
Physiol Rev 81, 1689-1723
   Abstract »    Full Text »    PDF »
The Golgi-Associated Hook3 Protein Is a Member of a Novel Family of Microtubule-Binding Proteins.
J. H. Walenta, A. J. Didier, X. Liu, and H. Kramer (2001)
J. Cell Biol. 152, 923-934
   Abstract »    Full Text »    PDF »
The Sar1 Gtpase Coordinates Biosynthetic Cargo Selection with Endoplasmic Reticulum Export Site Assembly.
M. Aridor, K. N. Fish, S. Bannykh, J. Weissman, T. H. Roberts, J. Lippincott-Schwartz, and W. E. Balch (2001)
J. Cell Biol. 152, 213-230
   Abstract »    Full Text »    PDF »
Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late Endosome-Lysosome Fusion.
B. M. Mullock, C. W. Smith, G. Ihrke, N. A. Bright, M. Lindsay, E. J. Parkinson, D. A. Brooks, R. G. Parton, D. E. James, J. P. Luzio, et al. (2000)
Mol. Biol. Cell 11, 3137-3153
   Abstract »    Full Text »
Role of p97 and Syntaxin 5 in the Assembly of Transitional Endoplasmic Reticulum.
L. Roy, J. J.M. Bergeron, C. Lavoie, R. Hendriks, J. Gushue, A. Fazel, A. Pelletier, D. J. Morré, V. N. Subramaniam, W. Hong, et al. (2000)
Mol. Biol. Cell 11, 2529-2542
   Abstract »    Full Text »
Rab1 Recruitment of p115 into a cis-SNARE Complex: Programming Budding COPII Vesicles for Fusion.
B. B. Allan, B. D. Moyer, and W. E. Balch (2000)
Science 289, 444-448
   Abstract »    Full Text »
Syntaxin 18, a SNAP Receptor That Functions in the Endoplasmic Reticulum, Intermediate Compartment, and cis-Golgi Vesicle Trafficking.
K. Hatsuzawa, H. Hirose, K. Tani, A. Yamamoto, R. H. Scheller, and M. Tagaya (2000)
J. Biol. Chem. 275, 13713-13720
   Abstract »    Full Text »    PDF »
Evidence for Overlapping and Distinct Functions in Protein Transport of Coat Protein Sec24p Family Members.
R. Peng, A. De Antoni, and D. Gallwitz (2000)
J. Biol. Chem. 275, 11521-11528
   Abstract »    Full Text »    PDF »
Asymmetric Requirements for a Rab Gtpase and Snare Proteins in Fusion of Copii Vesicles with Acceptor Membranes.
X. Cao and C. Barlowe (2000)
J. Cell Biol. 149, 55-66
   Abstract »    Full Text »    PDF »
The p58-positive pre-golgi intermediates consist of distinct subpopulations of particles that show differential binding of COPI and COPII coats and contain vacuolar H(+)-ATPase.
M Ying, T Flatmark, and J Saraste (2000)
J. Cell Sci. 113, 3623-3638
   Abstract »    PDF »
ER to Golgi Transport: Requirement for P115 at a Pre-Golgi Vtc Stage.
C. Alvarez, H. Fujita, A. Hubbard, and E. Sztul (1999)
J. Cell Biol. 147, 1205-1222
   Abstract »    Full Text »    PDF »
Protein Sorting by Directed Maturation of Golgi Compartments.
B. B. Allan and W. E. Balch (1999)
Science 285, 63-66
   Abstract »    Full Text »
A Role for Tlg1p in the Transport of Proteins within the Golgi Apparatus of Saccharomyces cerevisiae.
J. G. S. Coe, A. C. B. Lim, J. Xu, and W. Hong (1999)
Mol. Biol. Cell 10, 2407-2423
   Abstract »    Full Text »
A Di-acidic (DXE) Code Directs Concentration of Cargo during Export from the Endoplasmic Reticulum.
N. Nishimura, S. Bannykh, S. Slabough, J. Matteson, Y. Altschuler, K. Hahn, and W. E. Balch (1999)
J. Biol. Chem. 274, 15937-15946
   Abstract »    Full Text »    PDF »
Golgi Structure Correlates with Transitional Endoplasmic Reticulum Organization in Pichia pastoris and Saccharomyces cerevisiae.
O. W. Rossanese, J. Soderholm, B. J. Bevis, I. B. Sears, J. O'Connor, E. K. Williamson, and B. S. Glick (1999)
J. Cell Biol. 145, 69-81
   Abstract »    Full Text »    PDF »
The Specificity of Vesicle Trafficking: Coat Proteins and SNAREs.
A. A. Sanderfoot and N. V. Raikhel (1999)
PLANT CELL 11, 629-642
   Full Text »
Specific interaction of the yeast cis-Golgi syntaxin Sed5p and the coat protein complex II component Sec24p of endoplasmic reticulum-derived transport vesicles.
R. Peng, R. Grabowski, A. De Antoni, and D. Gallwitz (1999)
PNAS 96, 3751-3756
   Abstract »    Full Text »    PDF »
Morphological and Functional Association of Sec22b/ERS-24 with the pre-Golgi Intermediate Compartment.
T. Zhang, S. H. Wong, B. L. Tang, Y. Xu, and W. Hong (1999)
Mol. Biol. Cell 10, 435-453
   Abstract »    Full Text »
Membrane flow through the Golgi apparatus: specific disassembly of the cis-Golgi network by ATP depletion.
M del Valle, Y Robledo, and I. Sandoval (1999)
J. Cell Sci. 112, 4017-4029
   Abstract »    PDF »
Yeast ER-Golgi v-SNAREs Bos1p and Bet1p differ in steady-state localization and targeting.
D Ossipov, S Schroder-Kohne, and H. Schmitt (1999)
J. Cell Sci. 112, 4135-4142
   Abstract »    PDF »
Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network.
A. Valdez, J. Cabaniols, M. Brown, and P. Roche (1999)
J. Cell Sci. 112, 845-854
   Abstract »    PDF »
GS32, a Novel Golgi SNARE of 32 kDa, Interacts Preferentially with Syntaxin 6.
S. H. Wong, Y. Xu, T. Zhang, G. Griffiths, S. L. Lowe, V. N. Subramaniam, K. T. Seow, and W. Hong (1999)
Mol. Biol. Cell 10, 119-134
   Abstract »    Full Text »
Syntaxin 1A inhibits CFTR chloride channels by means of domain-specific protein-protein interactions.
A. P. Naren, M. W. Quick, J. F. Collawn, D. J. Nelson, and K. L. Kirk (1998)
PNAS 95, 10972-10977
   Abstract »    Full Text »    PDF »
The Dynamics of Golgi Protein Traffic Visualized in Living Yeast Cells.
S. Wooding and H. R.B. Pelham (1998)
Mol. Biol. Cell 9, 2667-2680
   Abstract »    Full Text »
Functional Reconstitution of Ypt7p GTPase and a Purified Vacuole SNARE Complex.
K. Sato and W. Wickner (1998)
Science 281, 700-702
   Abstract »    Full Text »
Localization, Dynamics, and Protein Interactions Reveal Distinct Roles for ER and Golgi SNAREs.
J. C. Hay, J. Klumperman, V. Oorschot, M. Steegmaier, C. S. Kuo, and R. H. Scheller (1998)
J. Cell Biol. 141, 1489-1502
   Abstract »    Full Text »    PDF »
Protein transport from the endoplasmic reticulum to the Golgi apparatus.
W Hong (1998)
J. Cell Sci. 111, 2831-2839
   Abstract »    PDF »
Kinase Signaling Initiates Coat Complex II (COPII) Recruitment and Export from the Mammalian Endoplasmic Reticulum.
M. Aridor and W. E. Balch (2000)
J. Biol. Chem. 275, 35673-35676
   Abstract »    Full Text »    PDF »
Subunit Structure of a Mammalian ER/Golgi SNARE Complex.
D. Xu, A. P. Joglekar, A. L. Williams, and J. C. Hay (2000)
J. Biol. Chem. 275, 39631-39639
   Abstract »    Full Text »    PDF »
Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28, and Bet1 and Participates in a Late Stage in Endoplasmic Reticulum-Golgi Transport.
T. Zhang and W. Hong (2001)
J. Biol. Chem. 276, 27480-27487
   Abstract »    Full Text »    PDF »
Tyrosine phosphorylation of p97 regulates transitional endoplasmic reticulum assembly in vitro.
C. Lavoie, E. Chevet, L. Roy, N. K. Tonks, A. Fazel, B. I. Posner, J. Paiement, and J. J. M. Bergeron (2000)
PNAS 97, 13637-13642
   Abstract »    Full Text »    PDF »
Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform.
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes (2002)
J. Cell Biol. 156, 653-664
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)