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Science 23 January 1998:
Vol. 279. no. 5350, pp. 573 - 577
DOI: 10.1126/science.279.5350.573
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Reports

Role of Dynamin in the Formation of Transport Vesicles from the Trans-Golgi Network

Steven M. Jones, Kathryn E. Howell, John R. Henley, Hong Cao, Mark A. McNiven *

Dynamin guanosine triphosphatases support the scission of clathrin-coated vesicles from the plasmalemma during endocytosis. By fluorescence microscopy of cultured rat hepatocytes, a green fluorescent protein-dynamin II fusion protein localized with clathrin-coated vesicles at the Golgi complex. A cell-free assay was utilized to demonstrate the role of dynamin in vesicle formation at the trans-Golgi. Addition of peptide-specific anti-dynamin antibodies to the assay mixture inhibited both constitutive exocytic and clathrin-coated vesicle formation. Immunodepletion of dynamin proteins also inhibited vesicle formation, and budding efficiency was restored upon readdition of purified dynamin. These data suggest that dynamin participates in the formation of distinct transport vesicles from the trans-Golgi network.

J. R. Henley, H. Cao, M. A. McNiven, Center for Basic Research in Digestive Diseases, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
S. M. Jones, K. E. Howell, Department of Cellular & Structural Biology, University of Colorado School of Medicine, 4200 East 9th Avenue, Denver, CO 80262, USA.
*   To whom correspondence should be addressed. E-mail: mcniven.mark{at}mayo.edu

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H Boleti, A Benmerah, D. Ojcius, N Cerf-Bensussan, and A Dautry-Varsat (1999)
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Redundant and Distinct Functions for Dynamin-1 and Dynamin-2 Isoforms.
Y. Altschuler, S. M. Barbas, L. J. Terlecky, K. Tang, S. Hardy, K. E. Mostov, and S. L. Schmid (1998)
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A. T. Fullerton, M.-Y. Bau, P. A. Conrad, and G. S. Bloom (1998)
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J.-P. Simon, T. Morimoto, V. A. Bankaitis, T. A. Gottlieb, I. E. Ivanov, M. Adesnik, and D. D. Sabatini (1998)
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Differential Distribution of Dynamin Isoforms in Mammalian Cells.
H. Cao, F. Garcia, and M. A. McNiven (1998)
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J. R. Henley, E. W.A. Krueger, B. J. Oswald, and M. A. McNiven (1998)
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J. Cell Sci. 111, 3451-3458
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Overexpression of Frequenin, a Modulator of Phosphatidylinositol 4-Kinase, Inhibits Biosynthetic Delivery of an Apical Protein in Polarized Madin-Darby Canine Kidney Cells.
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M. C. Hu, L. Fan, L. A. Crowder, Z. Karim-Jimenez, H. Murer, and O. W. Moe (2001)
J. Biol. Chem. 276, 26906-26915
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E. Harsay and R. Schekman (2002)
J. Cell Biol. 156, 271-286
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Dynamin-dependent Transferrin Receptor Recycling by Endosome-derived Clathrin-coated Vesicles.
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