Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 12 December 1997:
Vol. 278. no. 5345, pp. 1954 - 1957
DOI: 10.1126/science.278.5345.1954
Prev | Table of Contents | Next

Reports

Protein Disulfide Isomerase as a Regulator of Chloroplast Translational Activation

Jungmook Kim, Stephen P. Mayfield

Light-regulated translation of chloroplast messenger RNAs (mRNAs) requires trans-acting factors that interact with the 5' untranslated region (UTR) of these mRNAs. Chloroplast polyadenylate-binding protein (cPABP) specifically binds to the 5'-UTR of the psbA mRNA and is essential for translation of this mRNA. A protein disulfide isomerase that is localized to the chloroplast and copurifies with cPABP was shown to modulate the binding of cPABP to the 5'-UTR of the psbA mRNA by reversibly changing the redox status of cPABP through redox potential or adenosine 5'-diphosphate-dependent phosphorylation. This mechanism allows for a simple reversible switch regulating gene expression in the chloroplast.

Department of Cell Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Potential regulation of gene expression in photosynthetic cells by redox and energy state: approaches towards better understanding.
T. Pfannschmidt, K. Brautigam, R. Wagner, L. Dietzel, Y. Schroter, S. Steiner, and A. Nykytenko (2009)
Ann. Bot. 103, 599-607
   Abstract »    Full Text »    PDF »
Arabidopsis Protein Disulfide Isomerase-5 Inhibits Cysteine Proteases during Trafficking to Vacuoles before Programmed Cell Death of the Endothelium in Developing Seeds.
C. Andeme Ondzighi, D. A. Christopher, E. J. Cho, S.-C. Chang, and L. A. Staehelin (2008)
PLANT CELL 20, 2205-2220
   Abstract »    Full Text »    PDF »
Synthesis of the D2 Protein of Photosystem II in Chlamydomonas Is Controlled by a High Molecular Mass Complex Containing the RNA Stabilization Factor Nac2 and the Translational Activator RBP40.
C. Schwarz, I. Elles, J. Kortmann, M. Piotrowski, and J. Nickelsen (2007)
PLANT CELL 19, 3627-3639
   Abstract »    Full Text »    PDF »
Arabidopsis Cotyledon-Specific Chloroplast Biogenesis Factor CYO1 Is a Protein Disulfide Isomerase.
H. Shimada, M. Mochizuki, K. Ogura, J. E. Froehlich, K. W. Osteryoung, Y. Shirano, D. Shibata, S. Masuda, K. Mori, and K.-i. Takamiya (2007)
PLANT CELL 19, 3157-3169
   Abstract »    Full Text »    PDF »
The Chloroplast Protein Disulfide Isomerase RB60 Reacts with a Regulatory Disulfide of the RNA-binding Protein RB47.
T. Alergand, H. Peled-Zehavi, Y. Katz, and A. Danon (2006)
Plant Cell Physiol. 47, 540-548
   Abstract »    Full Text »    PDF »
Dual targeting of the protein disulfide isomerase RB60 to the chloroplast and the endoplasmic reticulum.
A. Levitan, T. Trebitsh, V. Kiss, Y. Pereg, I. Dangoor, and A. Danon (2005)
PNAS 102, 6225-6230
   Abstract »    Full Text »    PDF »
The Circadian Clock in Chlamydomonas reinhardtii. What Is It For? What Is It Similar To?.
M. Mittag, S. Kiaulehn, and C. H. Johnson (2005)
Plant Physiology 137, 399-409
   Full Text »    PDF »
Phylogenetic Analyses Identify 10 Classes of the Protein Disulfide Isomerase Family in Plants, Including Single-Domain Protein Disulfide Isomerase-Related Proteins.
N. L. Houston, C. Fan, Q.-Y. Xiang, J.-M. Schulze, R. Jung, and R. S. Boston (2005)
Plant Physiology 137, 762-778
   Abstract »    Full Text »    PDF »
RNA Binding Activity of the Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit from Chlamydomonas reinhardtii.
I. Yosef, V. Irihimovitch, J. A. Knopf, I. Cohen, I. Orr-Dahan, E. Nahum, C. Keasar, and M. Shapira (2004)
J. Biol. Chem. 279, 10148-10156
   Abstract »    Full Text »    PDF »
Light Control of Nuclear Gene mRNA Abundance and Translation in Tobacco.
L. Tang, S. Bhat, and M. E. Petracek (2003)
Plant Physiology 133, 1979-1990
   Abstract »    Full Text »
Decreased Content of Leaf Ferredoxin Changes Electron Distribution and Limits Photosynthesis in Transgenic Potato Plants.
S. Holtgrefe, K. P. Bader, P. Horton, R. Scheibe, A. von Schaewen, and J. E. Backhausen (2003)
Plant Physiology 133, 1768-1778
   Abstract »    Full Text »
Differential Regulation of Glucose-6-Phosphate Dehydrogenase Isoenzyme Activities in Potato.
R. Hauschild and A. von Schaewen (2003)
Plant Physiology 133, 47-62
   Abstract »    Full Text »    PDF »
A Mechanism for Light-Induced Translation of the rbcL mRNA Encoding the Large Subunit of Ribulose-1,5-bisphosphate Carboxylase in Barley Chloroplasts.
J. Kim and J. E. Mullet (2003)
Plant Cell Physiol. 44, 491-499
   Abstract »    Full Text »    PDF »
Human RNase H1 Activity Is Regulated by a Unique Redox Switch Formed between Adjacent Cysteines.
W. F. Lima, H. Wu, J. G. Nichols, S. M. Manalili, J. J. Drader, S. A. Hofstadler, and S. T. Crooke (2003)
J. Biol. Chem. 278, 14906-14912
   Abstract »    Full Text »    PDF »
DNA Microarray Analysis of Redox-Responsive Genes in the Genome of the Cyanobacterium Synechocystis sp. Strain PCC 6803.
Y. Hihara, K. Sonoike, M. Kanehisa, and M. Ikeuchi (2003)
J. Bacteriol. 185, 1719-1725
   Abstract »    Full Text »    PDF »
Identification of Mutants of Arabidopsis Defective in Acclimation of Photosynthesis to the Light Environment.
R. G. Walters, F. Shephard, J. J.M. Rogers, S. A. Rolfe, and P. Horton (2003)
Plant Physiology 131, 472-481
   Abstract »    Full Text »    PDF »
Expression and assembly of a fully active antibody in algae.
S. P. Mayfield, S. E. Franklin, and R. A. Lerner (2003)
PNAS 100, 438-442
   Abstract »    Full Text »    PDF »
The Active Site of the Thioredoxin-Like Domain of Chloroplast Protein Disulfide Isomerase, RB60, Catalyzes the Redox-Regulated Binding of Chloroplast Poly(A)-Binding Protein, RB47, to the 5' Untranslated Region of psbA mRNA.
J. Kim and S. P. Mayfield (2002)
Plant Cell Physiol. 43, 1238-1243
   Abstract »    Full Text »    PDF »
Characterization of Tbc2, a nucleus-encoded factor specifically required for translation of the chloroplast psbC mRNA in Chlamydomonas reinhardtii.
A. H. Auchincloss, W. Zerges, K. Perron, J. Girard-Bascou, and J.-D. Rochaix (2002)
J. Cell Biol. 157, 953-962
   Abstract »    Full Text »    PDF »
The 3'-Untranslated Region of Chloroplast psbA mRNA Stabilizes Binding of Regulatory Proteins to the Leader of the Message.
Y. S. Katz and A. Danon (2002)
J. Biol. Chem. 277, 18665-18669
   Abstract »    Full Text »    PDF »
Central Functions of the Lumenal and Peripheral Thylakoid Proteome of Arabidopsis Determined by Experimentation and Genome-Wide Prediction.
J.-B. Peltier, O. Emanuelsson, D. E. Kalume, J. Ytterberg, G. Friso, A. Rudella, D. A. Liberles, L. Soderberg, P. Roepstorff, G. von Heijne, et al. (2002)
PLANT CELL 14, 211-236
   Abstract »    Full Text »    PDF »
Cryptochrome 1, Cryptochrome 2, and Phytochrome A Co-Activate the Chloroplast psbD Blue Light-Responsive Promoter.
K. E. Thum, M. Kim, D. A. Christopher, and J. E. Mullet (2001)
PLANT CELL 13, 2747-2760
   Abstract »    Full Text »    PDF »
Adjacent cysteine residues as a redox switch.
C. Park and R. T. Raines (2001)
Protein Eng. Des. Sel. 14, 939-942
   Abstract »    Full Text »    PDF »
RNA Binding-Proteins Interact Specifically with the Arabidopsis Chloroplast psbA mRNA 5' Untranslated Region in a Redox-Dependent Manner.
Y. Shen, A. Danon, and D. A. Christopher (2001)
Plant Cell Physiol. 42, 1071-1078
   Abstract »    Full Text »    PDF »
Translation of chloroplast psbA mRNA is regulated by signals initiated by both photosystems II and I.
T. Trebitsh and A. Danon (2001)
PNAS
   Abstract »    Full Text »    PDF »
cis- and trans-Acting Determinants for Translation of psbD mRNA in Chlamydomonas reinhardtii.
F. Ossenbühl and J. Nickelsen (2000)
Mol. Cell. Biol. 20, 8134-8142
   Abstract »    Full Text »
Photosynthetic Electron Flow Regulates Transcription of the psaB Gene in Pea (Pisum sativum L.) Chloroplasts Through the Redox State of the Plastoquinone Pool.
A. Tullberg, K. Alexciev, T. Pfannschmidt, and J. F. Allen (2000)
Plant Cell Physiol. 41, 1045-1054
   Abstract »    Full Text »    PDF »
Biogenesis of the Chloroplast-Encoded D1 Protein: Regulation of Translation Elongation, Insertion, and Assembly into Photosystem II.
L. Zhang, V. Paakkarinen, K. J. van Wijk, and E.-M. Aro (2000)
PLANT CELL 12, 1769-1782
   Abstract »    Full Text »
Glutathione Redox Potential Modulated by Reactive Oxygen Species Regulates Translation of Rubisco Large Subunit in the Chloroplast.
V. Irihimovitch and M. Shapira (2000)
J. Biol. Chem. 275, 16289-16295
   Abstract »    Full Text »    PDF »
Sulfhydryl Regulation of L-Selectin Shedding: Phenylarsine Oxide Promotes Activation-Independent L-Selectin Shedding from Leukocytes.
T. A. Bennett, B. S. Edwards, L. A. Sklar, and S. Rogelj (2000)
J. Immunol. 164, 4120-4129
   Abstract »    Full Text »    PDF »
Disulfide Bond Formation between RNA Binding Domains Is Used to Regulate mRNA Binding Activity of the Chloroplast Poly(A)-binding Protein.
C. L. Fong, A. Lentz, and S. P. Mayfield (2000)
J. Biol. Chem. 275, 8275-8278
   Abstract »    Full Text »    PDF »
Translation of Chloroplast psbA mRNA Is Modulated in the Light by Counteracting Oxidizing and Reducing Activities.
T. Trebitsh, A. Levitan, A. Sofer, and A. Danon (2000)
Mol. Cell. Biol. 20, 1116-1123
   Abstract »    Full Text »
The Redox State Regulates RNA Degradation in the Chloroplast of Chlamydomonas reinhardtii.
M. L. Salvador and U. Klein (1999)
Plant Physiology 121, 1367-1374
   Abstract »    Full Text »
Two Isoforms of Protein Disulfide Isomerase Alter the Dimerization Status of E2A Proteins by a Redox Mechanism.
M. Markus and R. Benezra (1999)
J. Biol. Chem. 274, 1040-1049
   Abstract »    Full Text »    PDF »
A response regulator of cyanobacteria integrates diverse environmental signals and is critical for survival under extreme conditions.
R. Schwarz and A. R. Grossman (1998)
PNAS 95, 11008-11013
   Abstract »    Full Text »    PDF »
Light-dependent Formation of the Photosynthetic Proton Gradient Regulates Translation Elongation in Chloroplasts.
S. K. Muhlbauer and L. A. Eichacker (1998)
J. Biol. Chem. 273, 20935-20940
   Abstract »    Full Text »    PDF »
Translation of the Chloroplast psbA mRNA Requires the Nuclear-encoded Poly(A)-binding Protein, RB47.
C. B. Yohn, A. Cohen, C. Rosch, M. R. Kuchka, and S. P. Mayfield (1998)
J. Cell Biol. 142, 435-442
   Abstract »    Full Text »    PDF »
A poly(A) binding protein functions in the chloroplast as a message-specific translation factor.
C. B. Yohn, A. Cohen, A. Danon, and S. P. Mayfield (1998)
PNAS 95, 2238-2243
   Abstract »    Full Text »    PDF »
The Protein Disulfide Isomerase-like RB60 Is Partitioned between Stroma and Thylakoids in Chlamydomonas reinhardtii Chloroplasts.
T. Trebitsh, E. Meiri, O. Ostersetzer, Z. Adam, and A. Danon (2001)
J. Biol. Chem. 276, 4564-4569
   Abstract »    Full Text »    PDF »
Light-associated and Processing-dependent Protein Binding to 5' Regions of rbcL mRNA in the Chloroplasts of a C4 Plant.
D. J. McCormac, H. Litz, J. Wang, P. D. Gollnick, and J. O. Berry (2001)
J. Biol. Chem. 276, 3476-3483
   Abstract »    Full Text »    PDF »
A Novel Sugar-stimulated Covalent Switch in a Sugar Sensor.
Q. Chen, A. Nussbaum-Shochat, and O. Amster-Choder (2001)
J. Biol. Chem. 276, 44751-44756
   Abstract »    Full Text »    PDF »
Tracking the Light Environment by Cyanobacteria and the Dynamic Nature of Light Harvesting.
A. R. Grossman, D. Bhaya, and Q. He (2001)
J. Biol. Chem. 276, 11449-11452
   Full Text »    PDF »
Translation of chloroplast psbA mRNA is regulated by signals initiated by both photosystems II and I.
T. Trebitsh and A. Danon (2001)
PNAS 98, 12289-12294
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)