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Science 24 October 1997:
Vol. 278. no. 5338, pp. 695 - 698
DOI: 10.1126/science.278.5338.695
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Reports

Inhibition of HIV-1 Infection by the beta -Chemokine MDC

Ranajit Pal, Alfredo Garzino-Demo, Phillip D. Markham, Jennifer Burns, Michelle Brown, Robert C. Gallo, * Anthony L. DeVico

CD8+ T lymphocytes from individuals infected with human immunodeficiency virus-type 1 (HIV-1) secrete a soluble activity that suppresses infection by HIV-1. A protein associated with this activity was purified from the culture supernatant of an immortalized CD8+ T cell clone and identified as the beta -chemokine macrophage-derived chemokine (MDC). MDC suppressed infection of CD8+ cell-depleted peripheral blood mononuclear cells by primary non-syncytium-inducing and syncytium-inducing isolates of HIV-1 and the T cell line-adapted isolate HIV-1IIIB. MDC was expressed in activated, but not resting, peripheral blood mononuclear cells and binds a receptor on activated primary T cells. These observations indicate that beta -chemokines are responsible for a major proportion of HIV-1-specific suppressor activity produced by primary T cells.

R. Pal, P. D. Markham, M. Brown, Advanced BioScience Laboratories, 5510 Nicholson Lane, Kensington, MD 20895, USA.
A. Garzino-Demo, R. C. Gallo, A. L. DeVico, Institute of Human Virology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201-1192, USA.
J. Burns, Institute of Human Virology and Department of Microbiology and Immunology, University of Maryland School of Medicine, University of Maryland at Baltimore, 655 West Baltimore Street, Baltimore, MD 21201-1192, USA.
*   To whom correspondence should be addressed.

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