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Science 3 October 1997:
Vol. 278. no. 5335, pp. 106 - 109
DOI: 10.1126/science.278.5335.106
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Reports

Negative Regulation by HLA-DO of MHC Class II-Restricted Antigen Processing

Lisa K. Denzin, Derek B. Sant'Angelo, Craig Hammond, Michael J. Surman, Peter Cresswell *

HLA-DM is a major histocompatibility complex (MHC) class II-like molecule that facilitates antigen processing by catalyzing the exchange of invariant chain-derived peptides (CLIP) from class II molecules for antigenic peptides. HLA-DO is a second class II-like molecule that physically associates with HLA-DM in B cells. HLA-DO was shown to block HLA-DM function. Purified HLA-DM-DO complexes could not promote peptide exchange in vitro. Expression of HLA-DO in a class II+ and DM+, DO- human T cell line caused the accumulation of class II-CLIP complexes, indicating that HLA-DO blocked DM function in vivo and suggesting that HLA-DO is an important modulator of class II-restricted antigen processing.

Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, 310 Cedar Street, New Haven, CT 06510, USA.
*   To whom correspondence should be addressed. E-mail: peter.cresswell{at}qm.yale.edu

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