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Science 12 September 1997:
Vol. 277. no. 5332, pp. 1676 - 1681
DOI: 10.1126/science.277.5332.1676
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Reports

X-ray Structure of Bacteriorhodopsin at 2.5 Angstroms from Microcrystals Grown in Lipidic Cubic Phases

Eva Pebay-Peyroula, Gabriele Rummel, Jurg P. Rosenbusch, Ehud M. Landau

Lipidic cubic phases provide a continuous three-dimensional bilayer matrix that facilitates nucleation and growth of bacteriorhodopsin microcrystals. The crystals diffract x-rays isotropically to 2.0 angstroms. The structure of this light-driven proton pump was solved at a resolution of 2.5 angstroms by molecular replacement, using previous results from electron crystallographic studies as a model. The earlier structure was generally confirmed, but several differences were found, including loop conformations and side chain residues. Eight water molecules are now identified experimentally in the proton pathway. These findings reveal the constituents of the proton translocation pathway in the ground state.

E. Pebay-Peyroula, Institut de Biologie Structurale/CEA-CNRS/Université Joseph Fourier, 41 Avenue des Martyrs, F-38027 Grenoble Cedex 1, France.
G. Rummel, J. P. Rosenbusch, E. M. Landau, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.

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