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Science 12 September 1997:
Vol. 277. no. 5332, pp. 1672 - 1676
DOI: 10.1126/science.277.5332.1672
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Reports

Proteolysis and DNA Replication: The CDC34 Requirement in the Xenopus Egg Cell Cycle

P. Renee Yew, Marc W. Kirschner *

The cell division cycle gene, CDC34, is required for ubiquitin-mediated degradation of G1 regulators and cell cycle progression through the transition from G1 to S phase in budding yeast. A CDC34 requirement for S phase onset in higher eukaryotes has not been established. Studies of the simple embryonic cell cycle of Xenopus laevis eggs demonstrated that Cdc34p in a large molecular size complex was required in the initiation of DNA replication. Cdc34p appears to regulate the initiation function of Cdk2-cyclin E, perhaps through the degradation of the Xenopus cdk inhibitor, Xic1.

Department of Cell Biology, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.
*   To whom correspondence should be addressed. E-mail: marchms.harvard.edu

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