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Science 8 August 1997:
Vol. 277. no. 5327, pp. 834 - 836
DOI: 10.1126/science.277.5327.834
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Reports

Natural Behavior Polymorphism Due to a cGMP-Dependent Protein Kinase of Drosophila

K. A. Osborne, A. Robichon, E. Burgess, S. Butland, * R. A. Shaw, A. Coulthard, H. S. Pereira, dagger R. J. Greenspan, ddagger M. B. Sokolowski §

Naturally occuring polymorphisms in behavior are difficult to map genetically and thus are refractory to molecular characterization. An exception is the foraging gene (for), a gene that has two naturally occurring variants in Drosophila melanogaster food-search behavior: rover and sitter. Molecular mapping placed for mutations in the dg2 gene, which encodes a cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG). Rovers had higher PKG activity than sitters, and transgenic sitters expressing a dg2 complementary DNA from rover showed transformation of behavior to rover. Thus, PKG levels affected food-search behavior, and natural variation in PKG activity accounted for a behavioral polymorphism.

K. A. Osborne, E. Burgess, S. Butland, R. A. Shaw, A. Coulthard, H. S. Pereira, M. B. Sokolowski, Department of Biology, York University, 4700 Keele Street, North York, Toronto, Ontario, Canada, M3J 1P3.
A. Robichon and R. J. Greenspan, Department of Biology and Center for Neural Science, 1009 Main Building, New York University, New York, NY 10003, USA.
*   Present address: Department of Plant Science, University of British Columbia, 2357 Main Mall, Vancouver, British Columbia, Canada, V6T 1Z4.

dagger    Present address: Institute of Histology and Embryology, Faculty of Medicine, University of Lisbon, Avenue Professor, Egas Moniz, 1699 Lisboa Codex, Portugal.

ddagger    Present address: The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, CA 92121, USA.

§   To whom correspondence should be addressed. E-mail: mbsoko{at}yorku.ca

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