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Science 8 August 1997:
Vol. 277. no. 5327, pp. 825 - 828
DOI: 10.1126/science.277.5327.825
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Reports

Drosophila Mitotic Domain Boundaries as Cell Fate Boundaries

Sidney B. Cambridge, Robert L. Davis, Jonathan S. Minden *

Fate determination in Drosophila embryos is evidenced by the appearance of mitotic domains. To identify fate or fates of cells, individual cells in mitotic domains 2, 8, and 15 were marked and monitored through development. Comparison of the different fates indicated that domain boundaries are cell fate boundaries. Cells were marked by expression of GAL4-dependent transgenes after photoactivation of a caged GAL4VP16 analog that had its DNA binding activity inhibited with a photolabile blocking reagent. Caged GAL4VP16 was also used to induce gene expression in Xenopus embryos. Thus, photoactivated gene expression is a versatile tool for spatiotemporal control of gene expression.

S. B. Cambridge and J. S. Minden, Department of Biological Sciences and Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
R. L. Davis, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
*   To whom correspondence should be addressed. E-mail:minden{at}andrew.cmu.edu

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