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Science 25 July 1997:
Vol. 277. no. 5325, pp. 574 - 578
DOI: 10.1126/science.277.5325.574
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Reports

Mitosis in Living Budding Yeast: Anaphase A But No Metaphase Plate

Aaron F. Straight, * Wallace F. Marshall, John W. Sedat, Andrew W. Murray

Chromosome movements and spindle dynamics were visualized in living cells of the budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by expression of a protein fusion between green fluorescent protein (GFP) and the Lac repressor, which bound to an array of Lac operator binding sites integrated into the chromosome. Spindle microtubules were detected by expression of a protein fusion between GFP and Tub1, the major alpha tubulin. Spindle elongation and chromosome separation exhibited biphasic kinetics, and centromeres separated before telomeres. Budding yeast did not exhibit a conventional metaphase chromosome alignment but did show anaphase A, movement of the chromosomes to the poles.

A. F. Straight and A. W. Murray, Department of Physiology, Box 0444, School of Medicine, University of California at San Francisco, San Francisco, CA 94143-0444, USA.
W. F. Marshall and J. W. Sedat, Department of Biochemistry, Box 0448, School of Medicine, University of California at San Francisco, San Francisco, CA 94143-0448, USA.
*   To whom correspondence should be addressed.

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