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Science 25 July 1997:
Vol. 277. no. 5325, pp. 556 - 558
DOI: 10.1126/science.277.5325.556
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Reports

A Di-Acidic Signal Required for Selective Export from the Endoplasmic Reticulum

Noriyuki Nishimura, William E. Balch *

Transport of membrane proteins between intracellular compartments requires specific sequences in the protein cytoplasmic domain to direct packaging into vesicle shuttles. A sequence that mediates export from the endoplasmic reticulum (ER) has proved elusive. A di-acidic signal (Asp-X-Glu, where X represents any amino acid) on the cytoplasmic tail of vesicular stomatitis virus glycoprotein (VSV-G) and other cargo molecules was required for efficient recruitment to vesicles mediating export from the ER in baby hamster kidney cells. The existence of such a signal provides evidence that export from the ER occurs through a selective mechanism.

Departments of Molecular and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
*   To whom correspondence should be addressed. E-Mail: webalch{at}scripps.edu

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   Abstract »    PDF »
ERGIC-53 and traffic in the secretory pathway.
H. Hauri, F Kappeler, H Andersson, and C Appenzeller (2000)
J. Cell Sci. 113, 587-596
   Abstract »    PDF »
Efficient Export of the Vesicular Stomatitis Virus G Protein from the Endoplasmic Reticulum Requires a Signal in the Cytoplasmic Tail That Includes Both Tyrosine-based and Di-acidic Motifs.
C. S. Sevier, O. A. Weisz, M. Davis, and C. E. Machamer (2000)
Mol. Biol. Cell 11, 13-22
   Abstract »    Full Text »
Mannose-dependent Endoplasmic Reticulum (ER)-Golgi Intermediate Compartment-53-mediated ER to Golgi Trafficking of Coagulation Factors V and VIII.
M. Moussalli, S. W. Pipe, H.-P. Hauri, W. C. Nichols, D. Ginsburg, and R. J. Kaufman (1999)
J. Biol. Chem. 274, 32539-32542
   Abstract »    Full Text »    PDF »
Saturation of the Endoplasmic Reticulum Retention Machinery Reveals Anterograde Bulk Flow.
A. J. Crofts, N. Leborgne-Castel, S. Hillmer, D. G. Robinson, B. Phillipson, L. E. Carlsson, D. A. Ashford, and J. Denecke (1999)
PLANT CELL 11, 2233-2248
   Abstract »    Full Text »
A Di-acidic (DXE) Code Directs Concentration of Cargo during Export from the Endoplasmic Reticulum.
N. Nishimura, S. Bannykh, S. Slabough, J. Matteson, Y. Altschuler, K. Hahn, and W. E. Balch (1999)
J. Biol. Chem. 274, 15937-15946
   Abstract »    Full Text »    PDF »
Protein Targeting to Endoplasmic Reticulum by Dilysine Signals Involves Direct Retention in Addition to Retrieval.
H. Andersson, F. Kappeler, and H.-P. Hauri (1999)
J. Biol. Chem. 274, 15080-15084
   Abstract »    Full Text »    PDF »
The Transmembrane Domain of Hepatitis C Virus Glycoprotein E1 Is a Signal for Static Retention in the Endoplasmic Reticulum.
L. Cocquerel, S. Duvet, J.-C. Meunier, A. Pillez, R. Cacan, C. Wychowski, and J. Dubuisson (1999)
J. Virol. 73, 2641-2649
   Abstract »    Full Text »    PDF »
Mutation of the YXXL Endocytosis Motif in the Cytoplasmic Tail of Pseudorabies Virus gE.
R. S. Tirabassi and L. W. Enquist (1999)
J. Virol. 73, 2717-2728
   Abstract »    Full Text »    PDF »
The Endoplasmic Reticulum—Gateway of the Secretory Pathway.
A. Vitale and J. Denecke (1999)
PLANT CELL 11, 615-628
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Identification of Domains Mediating Transcriptional Activation and Cytoplasmic Export in the Caudal Homeobox Protein Cdx-3.
K. Y. Trinh, T. Jin, and D. J. Drucker (1999)
J. Biol. Chem. 274, 6011-6019
   Abstract »    Full Text »    PDF »


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