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Science 18 July 1997:
Vol. 277. no. 5324, pp. 381 - 383
DOI: 10.1126/science.277.5324.381
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Reports

In Vitro Propagation of the Prion-Like State of Yeast Sup35 Protein

Sergey V. Paushkin, Vitaly V. Kushnirov, Vladimir N. Smirnov, Michael D. Ter-Avanesyan *

The yeast cytoplasmically inherited genetic determinant [PSI+] is presumed to be a manifestation of the prion-like properties of the Sup35 protein (Sup35p). Here, cell-free conversion of Sup35p from [psi-] cells (Sup35ppsi-) to the prion-like [PSI+]-specific form (Sup35pPSI+) was observed. The conversion reaction could be repeated for several consecutive cycles, thus modeling in vitro continuous [PSI+] propagation. Size fractionation of lysates of [PSI+] cells demonstrated that the converting activity was associated solely with Sup35pPSI+ aggregates, which agrees with the nucleation model for [PSI+] propagation. Sup35pPSI+ was purified and showed high conversion activity, thus confirming the prion hypothesis for Sup35p.

Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, Moscow 121552, Russia.
*   To whom correspondence should be addressed. E-mail: vita{at}excard.msk.su

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