Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 11 July 1997:
Vol. 277. no. 5323, pp. 251 - 254
DOI: 10.1126/science.277.5323.251
Prev | Table of Contents | Next

Reports

Dictyostelium Development in the Absence of cAMP

Bin Wang, Adam Kuspa *

Adenosine 3',5'-monophosphate (cAMP) and cAMP-dependent protein kinase (PKA) are regulators of development in many organisms. Dictyostelium uses cAMP as an extracellular chemoattractant and as an intracellular signal for differentiation. Cells that are mutant in adenylyl cyclase do not develop. Moderate expression of the catalytic subunit of PKA in adenylyl cyclase-null cells led to near-normal development without detectable accumulation of cAMP. These results suggest that all intracellular cAMP signaling is effected through PKA and that signals other than extracellular cAMP coordinate morphogenesis in Dictyostelium.

Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA.
*   To whom correspondence should be addressed. E-mail: akuspa{at}bcm.tmc.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Loss of SMEK, a Novel, Conserved Protein, Suppresses mek1 Null Cell Polarity, Chemotaxis, and Gene Expression Defects.
M. C. Mendoza, F. Du, N. Iranfar, N. Tang, H. Ma, W. F. Loomis, and R. A. Firtel (2005)
Mol. Cell. Biol. 25, 7839-7853
   Abstract »    Full Text »    PDF »
Intracellular Role of Adenylyl Cyclase in Regulation of Lateral Pseudopod Formation during Dictyostelium Chemotaxis.
V. Stepanovic, D. Wessels, K. Daniels, W. F. Loomis, and D. R. Soll (2005)
Eukaryot. Cell 4, 775-786
   Abstract »    Full Text »    PDF »
CbfA, the C-Module DNA-Binding Factor, Plays an Essential Role in the Initiation of Dictyostelium discoideum Development.
T. Winckler, N. Iranfar, P. Beck, I. Jennes, O. Siol, U. Baik, W. F. Loomis, and T. Dingermann (2004)
Eukaryot. Cell 3, 1349-1358
   Abstract »    Full Text »    PDF »
Periodic Signaling Controlled by an Oscillatory Circuit That Includes Protein Kinases ERK2 and PKA.
M. Maeda, S. Lu, G. Shaulsky, Y. Miyazaki, H. Kuwayama, Y. Tanaka, A. Kuspa, and W. F. Loomis (2004)
Science 304, 875-878
   Abstract »    Full Text »    PDF »
A bZIP/bRLZ transcription factor required for DIF signaling in Dictyostelium.
C. R. L. Thompson, Q. Fu, C. Buhay, R. R. Kay, and G. Shaulsky (2004)
Development 131, 513-523
   Abstract »    Full Text »    PDF »
Genome-Wide Expression Analyses of Gene Regulation during Early Development of Dictyostelium discoideum.
N. Iranfar, D. Fuller, and W. F. Loomis (2003)
Eukaryot. Cell 2, 664-670
   Abstract »    Full Text »    PDF »
CulB, a Putative Ubiquitin Ligase Subunit, Regulates Prestalk Cell Differentiation and Morphogenesis in Dictyostelium spp..
B. Wang and A. Kuspa (2002)
Eukaryot. Cell 1, 126-136
   Abstract »    Full Text »    PDF »
Requirements for the adenylyl cyclases in the development of Dictyostelium.
C. Anjard, F. Soderbom, and W. F. Loomis (2001)
Development 128, 3649-3654
   Abstract »    Full Text »    PDF »
Regulated protein degradation controls PKA function and cell-type differentiation in Dictyostelium.
S. Mohanty, S. Lee, N. Yadava, M. J. Dealy, R. S. Johnson, and R. A. Firtel (2001)
Genes & Dev. 15, 1435-1448
   Abstract »    Full Text »    PDF »
From the Cover: How amoeboids self-organize into a fruiting body: Multicellular coordination in Dictyostelium discoideum.
A. F. M. Maree and P. Hogeweg (2001)
PNAS 98, 3879-3883
   Abstract »    Full Text »    PDF »
Cyclic Nucleotide Signaling in Toxoplasma gondii Bradyzoite Differentiation.
L. A. Kirkman, L. M. Weiss, and K. Kim (2001)
Infect. Immun. 69, 148-153
   Abstract »    Full Text »    PDF »
The Internal Phosphodiesterase RegA Is Essential for the Suppression of Lateral Pseudopods during Dictyostelium Chemotaxis.
D. J. Wessels, H. Zhang, J. Reynolds, K. Daniels, P. Heid, S. Lu, A. Kuspa, G. Shaulsky, W. F. Loomis, and D. R. Soll (2000)
Mol. Biol. Cell 11, 2803-2820
   Abstract »    Full Text »
A Novel Ras-interacting Protein Required for Chemotaxis and Cyclic Adenosine Monophosphate Signal Relay in Dictyostelium.
S. Lee, C. A. Parent, R. Insall, and R. A. Firtel (1999)
Mol. Biol. Cell 10, 2829-2845
   Abstract »    Full Text »
Cell-sorting in aggregates of Dictyostelium discoideum.
A Nicol, W Rappel, H Levine, and W. Loomis (1999)
J. Cell Sci. 112, 3923-3929
   Abstract »    PDF »
Role of PKA in the Timing of Developmental Events in Dictyostelium Cells.
W. F. Loomis (1998)
Microbiol. Mol. Biol. Rev. 62, 684-694
   Abstract »    Full Text »    PDF »
Non-autonomous regulation of a graded, PKA-mediated transcriptional activation signal for cell patterning.
P Balint-Kurti, G. Ginsburg, J Liu, and A. Kimmel (1998)
Development 125, 3947-3954
   Abstract »    PDF »
A novel Dictyostelium cell surface protein important for both cell adhesion and cell sorting.
R. Ginger, L Drury, C Baader, N. Zhukovskaya, and J. Williams (1998)
Development 125, 3343-3352
   Abstract »    PDF »
YakA, a protein kinase required for the transition from growth to development in Dictyostelium.
G. Souza, S Lu, and A Kuspa (1998)
Development 125, 2291-2302
   Abstract »    PDF »
A cAMP-phosphodiesterase controls PKA-dependent differentiation.
G Shaulsky, D Fuller, and W. Loomis (1998)
Development 125, 691-699
   Abstract »    PDF »
Cyclic AMP Inhibits Akt Activity by Blocking the Membrane Localization of PDK1.
S. Kim, K. Jee, D. Kim, H. Koh, and J. Chung (2001)
J. Biol. Chem. 276, 12864-12870
   Abstract »    Full Text »    PDF »
Light affects cAMP signaling and cell movement activity in Dictyostelium discoideum.
K. Miura and F. Siegert (2000)
PNAS 97, 2111-2116
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)