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Science 11 July 1997:
Vol. 277. no. 5323, pp. 221 - 225
DOI: 10.1126/science.277.5323.221
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Reports

Inducible Expression and Phosphorylation of Coactivator BOB.1/OBF.1 in T Cells

Stefan Zwilling, *dagger Andreas Dieckmann, dagger Petra Pfisterer, dagger Peter Angel, Thomas Wirth ddagger

BOB.1/OBF.1 is a transcriptional coactivator that is constitutively expressed in B cells and interacts with the Oct1 and Oct2 transcription factors. Upon activation of Jurkat T cells and primary murine thymocytes with phorbol esters and ionomycin, BOB.1/OBF.1 expression and transactivation function were induced. BOB.1/OBF.1 was phosphorylated at Ser184 both in vivo and in vitro, and this modification was required for inducible activation. Mutation of Ser184 also diminished transactivation function in B cells, suggesting that the activating phosphorylation that is inducible in T cells is constitutively present in B cells. Thus, BOB.1/OBF.1 is a transcriptional coactivator that is critically regulated by posttranslational modifications to mediate cell type-specific gene expression.

S. Zwilling, P. Pfisterer, T. Wirth, MSZ, Institut für Medizinische Strahlenkunde und Zellforschung, Universität Würzburg, Versbacher Straße 5, 97078 Würzburg, Germany.
A. Dieckmann and P. Angel, DKFZ, Deutsches Krebsforschungszentrum, Abteilung Signaltransduktion und Wachstumskontrolle, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
*   Present address: Novartis Pharma, Preclinical Research, Basel, Switzerland.

dagger    These authors contributed equally to this work.

ddagger    To whom correspondence should be addressed.

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