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Science 27 June 1997:
Vol. 276. no. 5321, pp. 2039 - 2042
DOI: 10.1126/science.276.5321.2039
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Reports

Exchange of Protein Molecules Through Connections Between Higher Plant Plastids

Rainer H. Köhler, Jun Cao, Warren R. Zipfel, Watt W. Webb, Maureen R. Hanson *

Individual plastids of vascular plants have generally been considered to be discrete autonomous entities that do not directly communicate with each other. However, in transgenic plants in which the plastid stroma was labeled with green fluorescent protein (GFP), thin tubular projections emanated from individual plastids and sometimes connected to other plastids. Flow of GFP between interconnected plastids could be observed when a single plastid or an interconnecting plastid tubule was photobleached and the loss of green fluorescence by both plastids was seen. These tubules allow the exchange of molecules within an interplastid communication system, which may facilitate the coordination of plastid activities.

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R. H. Köhler, J. Cao, M. R. Hanson, Section of Genetics and Development, Cornell University, Biotechnology Building, Ithaca, NY 14853-2703, USA.
W. R. Zipfel and W. W. Webb, School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853-2703, USA.
*   To whom correspondence should be addressed. E-mail: mrh5{at}cornell.edu

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