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Science 27 June 1997:
Vol. 276. no. 5321, pp. 2024 - 2027
DOI: 10.1126/science.276.5321.2024
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Reports

The Role of Antibody Concentration and Avidity in Antiviral Protection

M. F. Bachmann, * U. Kalinke, A. Althage, G. Freer, dagger C. Burkhart, ddagger H.-P. Roost, M. Aguet, H. Hengartner, R. M. Zinkernagel §

Neutralizing antibodies are necessary and sufficient for protection against infection with vesicular stomatitis virus (VSV). The in vitro neutralization capacities and in vivo protective capacities of a panel of immunoglobulin G monoclonal antibodies to the glycoprotein of VSV were evaluated. In vitro, neutralizing activity correlated with avidity and with neutralization rate constant, a measure of on-rate. However, in vivo, protection was independent of immunoglobulin subclass, avidity, neutralization rate constant, and in vitro neutralizing activity; above a minimal avidity threshold, protection depended simply on a minimum serum concentration. These two biologically defined thresholds of antibody specificity offer hope for the development of adoptive therapy with neutralizing antibodies.

M. F. Bachmann, U. Kalinke, A. Althage, G. Freer, C. Burkhart, H.-P. Roost, H. Hengartner, R. M. Zinkernagel, Institute of Experimental Immunology, Department of Pathology, University Hospital Zürich, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland.
M. Aguet, Swiss Cancer Institute, Chemin Des Boveresses 155, 1066 Epalinges, Switzerland.
*   Present address: Department for Medical Biophysics, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada.

dagger    Present address: Department of Biomedicine, University of Pisa, I-S6127 Pisa, Italy.

ddagger    Present address: Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, UK.

§   To whom correspondence should be addressed. E-mail: RZI{at}pty.smtp.usz.ch

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