Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 30 May 1997:
Vol. 276. no. 5317, pp. 1415 - 1418
DOI: 10.1126/science.276.5317.1415
Prev | Table of Contents | Next

Reports

Induction of Leaf Primordia by the Cell Wall Protein Expansin

Andrew J. Fleming, * Simon McQueen-Mason, Therese Mandel, Cris Kuhlemeier dagger

Expansins are extracellular proteins that increase plant cell wall extensibility in vitro. Beads loaded with purified expansin induced bulging on the leaf-generating organ, the apical meristem, of tomato plants. Some of these bulges underwent morphogenesis to produce leaflike structures, resulting in a reversal of the direction of phyllotaxis. Thus, expansin can induce tissue expansion in vivo, and localized control of tissue expansion may be sufficient to induce leaf formation. These results suggest a role for biophysical forces in the regulation of plant development.

A. J. Fleming, T. Mandel, C. Kuhlemeier, Institute of Plant Physiology, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.
S. McQueen-Mason, Department of Biology, The Plant Laboratory, University of York, York YO1 5YW, UK.
*   Present address: Institute of Plant Sciences, Eidgenössische Technische Hochschule-Zürich, CH-8092 Zürich, Switzerland.

dagger    To whom correspondence should be addressed. E-mail: kuhlemeier{at}pfp.unibe.ch

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Integration of transport-based models for phyllotaxis and midvein formation.
E. M. Bayer, R. S. Smith, T. Mandel, N. Nakayama, M. Sauer, P. Prusinkiewicz, and C. Kuhlemeier (2009)
Genes & Dev. 23, 373-384
   Abstract »    Full Text »    PDF »
Stress-driven buckling patterns in spheroidal core/shell structures.
J. Yin, Z. Cao, C. Li, I. Sheinman, and X. Chen (2008)
PNAS 105, 19132-19135
   Abstract »    Full Text »    PDF »
Pattern Selection in Plants: Coupling Chemical Dynamics to Surface Growth in Three Dimensions.
D. M. Holloway and L. G. Harrison (2008)
Ann. Bot. 101, 361-374
   Abstract »    Full Text »    PDF »
PLASTOCHRON2 Regulates Leaf Initiation and Maturation in Rice.
T. Kawakatsu, J.-I. Itoh, K. Miyoshi, N. Kurata, N. Alvarez, B. Veit, and Y. Nagato (2006)
PLANT CELL 18, 612-625
   Abstract »    Full Text »    PDF »
Lectin Receptor Kinases Participate in Protein-Protein Interactions to Mediate Plasma Membrane-Cell Wall Adhesions in Arabidopsis.
A. Gouget, V. Senchou, F. Govers, A. Sanson, A. Barre, P. Rouge, R. Pont-Lezica, and H. Canut (2006)
Plant Physiology 140, 81-90
   Abstract »    Full Text »    PDF »
Changes in expansin activity and gene expression during ethylene-promoted leaflet abscission in Sambucus nigra.
E. J. Belfield, B. Ruperti, J. A. Roberts, and S. McQueen-Mason (2005)
J. Exp. Bot. 56, 817-823
   Abstract »    Full Text »    PDF »
Microsurgical and laser ablation analysis of leaf positioning and dorsoventral patterning in tomato.
D. Reinhardt, M. Frenz, T. Mandel, and C. Kuhlemeier (2005)
Development 132, 15-26
   Abstract »    Full Text »    PDF »
Toward a Systems Approach to Understanding Plant Cell Walls.
C. Somerville, S. Bauer, G. Brininstool, M. Facette, T. Hamann, J. Milne, E. Osborne, A. Paredez, S. Persson, T. Raab, et al. (2004)
Science 306, 2206-2211
   Abstract »    Full Text »    PDF »
Structural integration at the shoot apical meristem: models, measurements, and experiments.
D. Kwiatkowska (2004)
Am. J. Botany 91, 1277-1293
   Abstract »    Full Text »    PDF »
Downregulation of the Petunia hybrida {alpha}-Expansin Gene PhEXP1 Reduces the Amount of Crystalline Cellulose in Cell Walls and Leads to Phenotypic Changes in Petal Limbs.
S. Zenoni, L. Reale, G. B. Tornielli, L. Lanfaloni, A. Porceddu, A. Ferrarini, C. Moretti, A. Zamboni, A. Speghini, F. Ferranti, et al. (2004)
PLANT CELL 16, 295-308
   Abstract »    Full Text »    PDF »
PLASTOCHRON1, a timekeeper of leaf initiation in rice, encodes cytochrome P450.
K. Miyoshi, B.-O. Ahn, T. Kawakatsu, Y. Ito, J.-I. Itoh, Y. Nagato, and N. Kurata (2004)
PNAS 101, 875-880
   Abstract »    Full Text »    PDF »
In Vivo Analysis of Cell Division, Cell Growth, and Differentiation at the Shoot Apical Meristem in Arabidopsis.
O. Grandjean, T. Vernoux, P. Laufs, K. Belcram, Y. Mizukami, and J. Traas (2004)
PLANT CELL 16, 74-87
   Abstract »    Full Text »    PDF »
Phyllotactic pattern and stem cell fate are determined by the Arabidopsis homeobox gene BELLRINGER.
M. E. Byrne, A. T. Groover, J. R. Fontana, and R. A. Martienssen (2003)
Development 130, 3941-3950
   Abstract »    Full Text »    PDF »
Microsurgical and laser ablation analysis of interactions between the zones and layers of the tomato shoot apical meristem.
D. Reinhardt, M. Frenz, T. Mandel, and C. Kuhlemeier (2003)
Development 130, 4073-4083
   Abstract »    Full Text »    PDF »
Regulation of Expansin Gene Expression Affects Growth and Development in Transgenic Rice Plants.
D. Choi, Y. Lee, H.-T. Cho, and H. Kende (2003)
PLANT CELL 15, 1386-1398
   Abstract »    Full Text »
Manipulation of leaf shape by modulation of cell division.
J. Wyrzykowska, S. Pien, W. H. Shen, and A. J. Fleming (2003)
Development 129, 957-964
   Abstract »    Full Text »    PDF »
Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean.
D.-K. Lee, J. H. Ahn, S.-K. Song, Y. D. Choi, and J. S. Lee (2003)
Plant Physiology 131, 985-997
   Abstract »    Full Text »    PDF »
The Growing World of Expansins.
D. J. Cosgrove, L. C. Li, H.-T. Cho, S. Hoffmann-Benning, R. C. Moore, and D. Blecker (2002)
Plant Cell Physiol. 43, 1436-1444
   Abstract »    Full Text »    PDF »
Signalling in Plant Lateral Organ Development.
J. F. Golz and A. Hudson (2002)
PLANT CELL 14, S277-288
   Full Text »    PDF »
Dancing Together. Social Controls in Parasitic Plant Development.
W. J. Keyes, J. V. Taylor, R. P. Apkarian, and D. G. Lynn (2001)
Plant Physiology 127, 1508-1512
   Full Text »    PDF »
Two Tomato Expansin Genes Show Divergent Expression and Localization in Embryos during Seed Development and Germination.
F. Chen, P. Dahal, and K. J. Bradford (2001)
Plant Physiology 127, 928-936
   Abstract »    Full Text »    PDF »
Extracellular Calmodulin Stimulates Light-Independent RbcS-GUS Expression in Suspension-Cultured Cells of Transgenic Tobacco.
J. Zhou, L. Ma, S. Zhang, Y. Zhu, and D. Sun (2001)
Plant Cell Physiol. 42, 1049-1055
   Abstract »    Full Text »    PDF »
Unlocking the mysteries of leaf primordia formation.
R. E. Cleland (2001)
PNAS 98, 10981-10982
   Full Text »    PDF »
Local expression of expansin induces the entire process of leaf development and modifies leaf shape.
S. Pien, J. Wyrzykowska, S. McQueen-Mason, C. Smart, and A. Fleming (2001)
PNAS
   Abstract »    Full Text »    PDF »
Expression of six expansin genes in relation to extension activity in developing strawberry fruit.
E. P. Harrison, S. J. McQueen-Mason, and K. Manning (2001)
J. Exp. Bot. 52, 1437-1446
   Abstract »    Full Text »    PDF »
Antisense Expression of a Cell Wall-Associated Protein Kinase, WAK4, Inhibits Cell Elongation and Alters Morphology.
D. Lally, P. Ingmire, H.-Y. Tong, and Z.-H. He (2001)
PLANT CELL 13, 1317-1332
   Abstract »    Full Text »    PDF »
Wall-Associated Kinases Are Expressed throughout Plant Development and Are Required for Cell Expansion.
T. A. Wagner and B. D. Kohorn (2001)
PLANT CELL 13, 303-318
   Abstract »    Full Text »
Expression of an Expansin Is Associated with Endosperm Weakening during Tomato Seed Germination.
F. Chen and K. J. Bradford (2000)
Plant Physiology 124, 1265-1274
   Abstract »    Full Text »
Adaptation of roots to low water potentials by changes in cell wall extensibility and cell wall proteins.
Y. Wu and D. J. Cosgrove (2000)
J. Exp. Bot. 51, 1543-1553
   Abstract »    Full Text »    PDF »
Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana.
H.-T. Cho and D. J. Cosgrove (2000)
PNAS
   Abstract »    Full Text »
Expansin Message Regulation in Parasitic Angiosperms: Marking Time in Development.
R. C. O'Malley and D. G. Lynn (2000)
PLANT CELL 12, 1455-1466
   Abstract »    Full Text »
Limited Correlation between Expansin Gene Expression and Elongation Growth Rate.
D. Caderas, M. Muster, H. Vogler, T. Mandel, J. K.C. Rose, S. McQueen-Mason, and C. Kuhlemeier (2000)
Plant Physiology 123, 1399-1414
   Abstract »    Full Text »
Subcellular Localization of Expansin mRNA in Xylem Cells.
K.-H. Im, D. J. Cosgrove, and A. M. Jones (2000)
Plant Physiology 123, 463-470
   Abstract »    Full Text »
A glycoconjugate from corms of saffron plant (Crocus sativus L.) inhibits root growth and affects in vitro cell viability.
J. A. Fernandez, J. Escribano, A. Piqueras, and J. Medina (2000)
J. Exp. Bot. 51, 731-737
   Abstract »    Full Text »    PDF »
Auxin Regulates the Initiation and Radial Position of Plant Lateral Organs.
D. Reinhardt, T. Mandel, and C. Kuhlemeier (2000)
PLANT CELL 12, 507-518
   Abstract »    Full Text »
An Expansin Gene Expressed in Ripening Strawberry Fruit.
P. M. Civello, A. L.T. Powell, A. Sabehat, and A. B. Bennett (1999)
Plant Physiology 121, 1273-1279
   Abstract »    Full Text »
Expression of pattern in plants: combining molecular and calculus-based biophysical paradigms.
P. B Green (1999)
Am. J. Botany 86, 1059-1076
   Abstract »    Full Text »    PDF »
Members of the YABBY gene family specify abaxial cell fate in Arabidopsis.
K. Siegfried, Y Eshed, S. Baum, D Otsuga, G. Drews, and J. Bowman (1999)
Development 126, 4117-4128
   Abstract »    PDF »
Control of phyllotaxy in maize by the abphyl1 gene.
D Jackson and S Hake (1999)
Development 126, 315-323
   Abstract »    PDF »
Cell Wall Loosening by Expansins.
D. J. Cosgrove (1998)
Plant Physiology 118, 333-339
   Full Text »
Leaf Initiation: New Developments in an Expanding Field.
B. Veit (1998)
PLANT CELL 10, 1407-1412
   Full Text »
Localized Upregulation of a New Expansin Gene Predicts the Site of Leaf Formation in the Tomato Meristem.
D. Reinhardt, F. Wittwer, T. Mandel, and C. Kuhlemeier (1998)
PLANT CELL 10, 1427-1438
   Abstract »    Full Text »
Spatial and Temporal Analyses of Expansion and Cell Cycle in Sunflower Leaves . A Common Pattern of Development for All Zones of a Leaf and Different Leaves of a Plant.
C. Granier and F. Tardieu (1998)
Plant Physiology 116, 991-1001
   Abstract »    Full Text »
Localized changes in apoplastic and cytoplasmic pH are associated with root hair development in Arabidopsis thaliana.
T. Bibikova, T Jacob, I Dahse, and S Gilroy (1998)
Development 125, 2925-2934
   Abstract »    PDF »
Symplasmic fields in the tunica of the shoot apical meristem coordinate morphogenetic events.
P. Rinne and C van der Schoot (1998)
Development 125, 1477-1485
   Abstract »    PDF »
From the Cover: Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana.
H.-T. Cho and D. J. Cosgrove (2000)
PNAS 97, 9783-9788
   Abstract »    Full Text »    PDF »
From the Cover: Local expression of expansin induces the entire process of leaf development and modifies leaf shape.
S. Pien, J. Wyrzykowska, S. McQueen-Mason, C. Smart, and A. Fleming (2001)
PNAS 98, 11812-11817
   Abstract »    Full Text »    PDF »
Plant Expansins Are a Complex Multigene Family with an Ancient Evolutionary Origin.
Y. Li, C. P. Darley, V. Ongaro, A. Fleming, O. Schipper, S. L. Baldauf, and S. J. McQueen-Mason (2002)
Plant Physiology 128, 854-864
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)