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Science 30 May 1997:
Vol. 276. no. 5317, pp. 1412 - 1415
DOI: 10.1126/science.276.5317.1412
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Reports

A Cellular Cofactor for the Constitutive Transport Element of Type D Retrovirus

Hengli Tang, Guido M. Gaietta, Wolfgang H. Fischer, Mark H. Ellisman, Flossie Wong-Staal *

A human nuclear protein that specifically interacts with the constitutive transport element (CTE) of simian retrovirus was identified as adenosine 5'-triphosphate-dependent RNA helicase A. This protein could bind to functional CTE but not to inactive CTE mutants. The interaction of helicase A with CTE was distinct from previously described helicase activity of this protein. Helicase A shuttled from the nucleus to the cytoplasm in the presence of a transcription inhibitor or in cells transiently overexpressing CTE-containing RNA. In vivo colocalization of helicase A and CTE was observed in experiments that combined in situ hybridization and immunostaining. These results suggest that helicase A plays a role in the nuclear export of CTE-containing RNA.

H. Tang, Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.
G. Gaietta and M. H. Ellisman, Department of Neurosciences, and San Diego Microscopy Imaging Resource, University of California, San Diego, La Jolla, CA 92093, USA.
W. H. Fischer, Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, CA 92097, USA.
F. Wong-Staal, Department of Medicine and Biology, University of California, San Diego, La Jolla, CA 92093-0665, USA.
*   To whom correspondence should be addressed.

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