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Science 23 May 1997:
Vol. 276. no. 5316, pp. 1255 - 1258
DOI: 10.1126/science.276.5316.1255
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Reports

t-SNARE Activation Through Transient Interaction with a Rab-Like Guanosine Triphosphatase

Vladimir V. Lupashin and M. Gerard Waters *

Intracellular vesicle targeting involves the interaction of vesicle proteins, termed v-SNAREs, with target membrane proteins, termed t-SNAREs. Assembly of v-SNARE-t-SNARE targeting complexes is modulated by members of the Sec1-Sly1 protein family, and by small guanosine triphosphatases termed Rabs. The interactions of these proteins during assembly of the endoplasmic reticulum-to-Golgi targeting complex in Saccharomyces cerevisiae were studied. The data suggest that the Rab protein Ypt1p transiently interacts with the t-SNARE Sed5p and results in displacement of the negative regulator Sly1p, allowing subsequent formation of the v-SNARE-t-SNARE targeting complex.

Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
*   To whom correspondence should be addressed. E-mail: gwaters{at}molbiol.princeton.edu

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