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Science 9 May 1997:
Vol. 276. no. 5314, pp. 930 - 932
DOI: 10.1126/science.276.5314.930
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Reports

Polarization-Enhanced NMR Spectroscopy of Biomolecules in Frozen Solution

Dennis A. Hall, Douglas C. Maus, Gary J. Gerfen, Souheil J. Inati, Lino R. Becerra, * Frederick W. Dahlquist, Robert G. Griffin dagger

Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

D. A. Hall, D. C. Maus, G. J. Gerfen, L. R. Becerra, R. G. Griffin, Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
S. J. Inati, Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
F. W. Dahlquist, Department of Chemistry, University of Oregon, Eugene, OR 97403, USA.
*   Present address: Massachusetts General Hospital-NMR Center, Massachusetts General Hospital, Charlestown, MA 02129, USA.

dagger    To whom correspondence should be addressed. E-mail: griffin{at}ccnmr.mit.edu

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