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This article has been retracted

Science 18 April 1997:
Vol. 276. no. 5311, pp. 407 - 409
DOI: 10.1126/science.276.5311.407
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Reports

Endosomal Targeting by the Cytoplasmic Tail of Membrane Immunoglobulin

Peter Weiser, *dagger Ralph Müller, * Uschi Braun, Michael Reth ddagger

Membrane-bound immunoglobulin (mIg) of the IgG, IgA, and IgE classes have conserved cytoplasmic tails. To investigate the function of these tails, a B cell line was transfected with truncated or mutated gamma 2a heavy chains. Transport to the endosomal compartment of antigen bound by the B cell antigen receptor did not occur in the absence of the cytoplasmic tail; and one or two mutations, respectively, in the Tyr-X-X-Met motif of the tail partially or completely interrupted the process. Experiments with chimeric antigen receptors confirmed these findings. Thus, a role for the cytoplasmic tail of mIg heavy chains in endosomal targeting of antigen is revealed.

Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-79108 Freiburg, Germany.
*   These authors contributed equally to this work.

dagger    Present address: Institut Curie, 12 rue Lhomond, 75005 Paris, France.

ddagger    To whom correspondence should be addressed. E-mail: reth{at}immunbio.mpg.de

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