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Science 14 March 1997:
Vol. 275. no. 5306, pp. 1643 - 1648
DOI: 10.1126/science.275.5306.1643
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Reports

Spatially and Functionally Distinct Ca2+ Stores in Sarcoplasmic and Endoplasmic Reticulum

Vera A. Golovina and Mordecai P. Blaustein *

The organization of calcium (Ca2+) stores in the sarcoplasmic and endoplasmic reticulum (S-ER) is poorly understood. The dynamics of the storage and release of calcium in the S-ER of intact, cultured astrocytes and arterial myocytes were studied with high-resolution imaging methods. The S-ER appeared to be a continuous tubular network; nevertheless, calcium stores in the S-ER were organized into small, spatially distinct compartments that functioned as discrete units. Cyclopiazonic acid (an inhibitor of the calcium pump in the S-ER membrane) and caffeine or ryanodine unloaded different, spatially separate compartments. Heterogeneity of calcium stores was also revealed in cells activated by physiological agonists. These results suggest that cells can generate spatially and temporally distinct calcium signals to control individual calcium-dependent processes.

Department of Physiology and Center for Vascular Biology and Hypertension, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
*   To whom correspondence should be addressed.

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Y. Imaizumi, Y. Torii, Y. Ohi, N. Nagano, K. Atsuki, H. Yamamura, K. Muraki, M. Watanabe, and T. B Bolton (1998)
J. Physiol. 510, 705-719
   Abstract »    Full Text »    PDF »
AMPA and NMDA Receptors Expressed by Differentiating Xenopus Spinal Neurons.
E. L. Gleason and N. C. Spitzer (1998)
J Neurophysiol 79, 2986-2998
   Abstract »    Full Text »    PDF »
Hypoxia and smooth muscle function: key regulatory events during metabolic stress.
M. J Taggart and S. Wray (1998)
J. Physiol. 509, 315-325
   Abstract »    Full Text »    PDF »
Calcium-dependent Clustering of Inositol 1,4,5-Trisphosphate Receptors.
B. S. Wilson, J. R. Pfeiffer, A. J. Smith, J. M. Oliver, J. A. Oberdorf, and R. J.H. Wojcikiewicz (1998)
Mol. Biol. Cell 9, 1465-1478
   Abstract »    Full Text »
Aggrecan Synthesis and Secretion. A PARADIGM FOR MOLECULAR AND CELLULAR COORDINATION OF MULTIGLOBULAR PROTEIN FOLDING AND INTRACELLULAR TRAFFICKING.
J. Zheng, W. Luo, and M. L. Tanzer (1998)
J. Biol. Chem. 273, 12999-13006
   Abstract »    Full Text »    PDF »
A continuum of InsP3-mediated elementary Ca2+ signalling events in Xenopus oocytes.
X.-P. Sun, N. Callamaras, J. S Marchant, and I. Parker (1998)
J. Physiol. 509, 67-80
   Abstract »    Full Text »    PDF »
Variability in spontaneous subcellular calcium release in guinea-pig ileum smooth muscle cells.
D V Gordienko, T B Bolton, and M B Cannell (1998)
J. Physiol. 507, 707-720
   Abstract »    Full Text »    PDF »


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