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Science 28 February 1997:
Vol. 275. no. 5304, pp. 1311 - 1314
DOI: 10.1126/science.275.5304.1311
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Reports

APC-Mediated Proteolysis of Ase1 and the Morphogenesis of the Mitotic Spindle

Yue-Li Juang, James Huang, Jan-Michael Peters, * Margaret E. McLaughlin, Chin-Yin Tai, David Pellman dagger

The molecular mechanisms that link cell-cycle controls to the mitotic apparatus are poorly understood. A component of the Saccharomyces cerevisiae spindle, Ase1, was observed to undergo cell cycle-specific degradation mediated by the cyclosome, or anaphase promoting complex (APC). Ase1 was degraded when cells exited from mitosis and entered G1. Inappropriate expression of stable Ase1 during G1 produced a spindle defect that is sensed by the spindle assembly checkpoint. In addition, loss of ASE1 function destabilized telophase spindles, and expression of a nondegradable Ase1 mutant delayed spindle disassembly. APC-mediated proteolysis therefore appears to regulate both spindle assembly and disassembly.

Y.-L. Juang, J. Huang, M. E. McLaughlin, C.-Y. Tai, D. Pellman, Department of Pediatric Oncology, The Dana-Farber Cancer Institute, and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
J.-M. Peters, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
*   Present address: Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.

dagger    To whom correspondence should be addressed. E-mail: david_pellman{at}dfci.harvard.edu

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