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Science 14 February 1997:
Vol. 275. no. 5302, pp. 980 - 983
DOI: 10.1126/science.275.5302.980
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Reports

Structural and Functional Conservation of the Caenorhabditis elegans Timing Gene clk-1

Jonathan J. Ewbank, Thomas M. Barnes, Bernard Lakowski, Marc Lussier, Howard Bussey, Siegfried Hekimi *

Mutations in the Caenorhabditis elegans gene clk-1 affect biological timing and extend longevity. The gene clk-1 was identified, and the cloned gene complemented the clk-1 phenotypes and restored normal longevity. The CLK-1 protein was found to be conserved among eukaryotes, including humans, and structurally similar to the yeast metabolic regulator Cat5p (also called Coq7p). These proteins contain a tandem duplication of a core 82-residue domain. clk-1 complemented the phenotype of cat5/coq7 null mutants, demonstrating that clk-1 and CAT5/COQ7 share biochemical function and that clk-1 acts at the level of cellular physiology.

Department of Biology, McGill University, 1205 Avenue Docteur Penfield, Montréal, Québec, Canada H3A 1B1.
*   To whom correspondence should be addressed. E-mail: hekimi{at}notung.biol.mcgill.ca

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Science. ISSN 0036-8075 (print), 1095-9203 (online)