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Science 31 January 1997:
Vol. 275. no. 5300, pp. 678 - 683
DOI: 10.1126/science.275.5300.678
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Reports

Positive Selection of T Cells Induced by Viral Delivery of Neopeptides to the Thymus

Naoko Nakano, Ronald Rooke, Christophe Benoist, Diane Mathis

The relation between an antigenic peptide that can stimulate a mature T cell and the natural peptide that promoted selection of this cell in the thymus is still unknown. An experimental system was devised to address this issue in vivo---mice expressing neopeptides in thymic stromal cells after adenovirus-mediated delivery of invariant chain-peptide fusion proteins. In this system, selection of T cells capable of responding to a given antigenic peptide could be promoted by the peptide itself, by closely related analogs lacking agonist and antagonist activity, or by ostensibly unrelated peptides. However, the precise repertoire of T cells selected was dictated by the particular neopeptide expressed.

Institut de Génétique et de Biologie Moléculaire et Cellulaire (INSERM, CNRS, Université Louis Pasteur), 1 rue Laurent Fries, 67404 Illkirch, C.U. de Strasbourg, France.

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Molecular Mimicry and T Cell-mediated Autoimmune Disease.
V. Barnaba and F. Sinigaglia (1997)
J. Exp. Med. 185, 1529-1532
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Paradoxical intrathymic positive selection in mice with only a covalently presented agonist peptide.
C. Viret, X. He, and C. A. Janeway Jr. (2001)
PNAS 98, 9243-9248
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Diversity of T cell repertoire shaped by a single peptide ligand is critically affected by its amino acid residue at a T cell receptor contact.
Y. Fukui, T. Oono, J.-P. Cabaniols, K. Nakao, K. Hirokawa, A. Inayoshi, T. Sanui, J. Kanellopoulos, E. Iwata, M. Noda, et al. (2000)
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