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Science 24 January 1997:
Vol. 275. no. 5299, pp. 543 - 547
DOI: 10.1126/science.275.5299.543
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Reports

Still life, a Protein in Synaptic Terminals of Drosophila Homologous to GDP-GTP Exchangers

Masaki Sone, Mikio Hoshino, * Emiko Suzuki, Shinya Kuroda, Kozo Kaibuchi, Hideki Nakagoshi, Kaoru Saigo, Yo-ichi Nabeshima, Chihiro Hama dagger

The morphology of axon terminals changes with differentiation into mature synapses. A molecule that might regulate this process was identified by a screen of Drosophila mutants for abnormal motor activities. The still life (sif) gene encodes a protein homologous to guanine nucleotide exchange factors, which convert Rho-like guanosine triphosphatases (GTPases) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The SIF proteins are found adjacent to the plasma membrane of synaptic terminals. Expression of a truncated SIF protein resulted in defects in neuronal morphology and induced membrane ruffling with altered actin localization in human KB cells. Thus, SIF proteins may regulate synaptic differentiation through the organization of the actin cytoskeleton by activating Rho-like GTPases.

M. Sone, Department of Molecular Genetics, National Institute of Neuroscience (NIN), National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, and Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.
M. Hoshino, H. Nakagoshi, Y.-i. Nabeshima, C. Hama, Department of Molecular Genetics, NIN, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187, Japan.
E. Suzuki, Department of Fine Morphology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan.
S. Kuroda and K. Kaibuchi, Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-01, Japan.
K. Saigo, Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.
*   Present address: Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305-5401, USA.

dagger    To whom correspondence should be addressed.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)