Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 24 January 1997:
Vol. 275. no. 5299, pp. 530 - 532
DOI: 10.1126/science.275.5299.530
Prev | Table of Contents | Next

Reports

Measuring Serotonin Distribution in Live Cells with Three-Photon Excitation

S. Maiti, Jason B. Shear, * R. M. Williams, W. R. Zipfel, Watt W. Webb dagger

Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration (sim 50 mM) and content (up to sim 5 × 108 molecules) of individual secretory granules.

School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
All authors contributed equally to this work.
*   Present address: Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA.

dagger    To whom correspondence should be addressed.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Serotonin in pre-implantation mouse embryos is localized to the mitochondria and can modulate mitochondrial potential.
B. Basu, R. Desai, J Balaji, R. Chaerkady, V Sriram, S. Maiti, and M. M Panicker (2008)
Reproduction 135, 657-669
   Abstract »    Full Text »    PDF »
Principles of Multiphoton-Excitation Fluorescence Microscopy.
W. Denk (2007)
CSH Protocols 2007, pdb.top23
   Abstract »    Full Text »
Dynamic Multiphoton Imaging: A Live View from Cells to Systems.
G. E. Stutzmann and I. Parker (2005)
Physiology 20, 15-21
   Abstract »    Full Text »    PDF »
Flavoprotein Autofluorescence Imaging of Neuronal Activation in the Cerebellar Cortex In Vivo.
K. C. Reinert, R. L. Dunbar, W. Gao, G. Chen, and T. J. Ebner (2004)
J Neurophysiol 92, 199-211
   Abstract »    Full Text »    PDF »
Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.
W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb (2003)
PNAS 100, 7075-7080
   Abstract »    Full Text »    PDF »
Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis.
K. Wolf, I. Mazo, H. Leung, K. Engelke, U. H. von Andrian, E. I. Deryugina, A. Y. Strongin, E.-B. Brocker, and P. Friedl (2003)
J. Cell Biol. 160, 267-277
   Abstract »    Full Text »    PDF »
From the Cover: Nondiffusive mechanisms enhance protein uptake rates in ion exchange particles.
S. R. Dziennik, E. B. Belcher, G. A. Barker, M. J. DeBergalis, S. E. Fernandez, and A. M. Lenhoff (2003)
PNAS 100, 420-425
   Abstract »    Full Text »    PDF »
Two-photon excitation fluorescence imaging of the living juxtaglomerular apparatus.
J. Peti-Peterdi, S. Morishima, P. D. Bell, and Y. Okada (2002)
Am J Physiol Renal Physiol 283, F197-F201
   Abstract »    Full Text »    PDF »
High Density Lipoprotein-mediated Cholesterol Uptake and Targeting to Lipid Droplets in Intact L-cell Fibroblasts. A SINGLE- AND MULTIPHOTON FLUORESCENCE APPROACH.
A. Frolov, A. Petrescu, B. P. Atshaves, P. T. C. So, E. Gratton, G. Serrero, and F. Schroeder (2000)
J. Biol. Chem. 275, 12769-12780
   Abstract »    Full Text »    PDF »
Single granule pH cycling in antigen-induced mast cell secretion.
R. Williams and W. Webb (2000)
J. Cell Sci. 113, 3839-3850
   Abstract »    PDF »
Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes.
J. A. Lundqvist, F. Sahlin, M. A. I. Aberg, A. Stromberg, P. S. Eriksson, and O. Orwar (1998)
PNAS 95, 10356-10360
   Abstract »    Full Text »    PDF »
Assaying for peptides in individual Aplysia neurons with mass spectrometry.
D. T. Chiu and R. N. Zare (1998)
PNAS 95, 3338-3340
   Full Text »    PDF »
Stimulated release of fluorescently labeled IgE fragments that efficiently accumulate in secretory granules after endocytosis in RBL-2H3 mast cells.
K Xu, R. Williams, D Holowka, and B Baird (1998)
J. Cell Sci. 111, 2385-2396
   Abstract »    PDF »
Fluorescence correlation spectroscopy: Diagnostics for sparse molecules.
S. Maiti, U. Haupts, and W. W. Webb (1997)
PNAS 94, 11753-11757
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)