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Science 3 January 1997:
Vol. 275. no. 5296, pp. 70 - 73
DOI: 10.1126/science.275.5296.70
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Reports

Microtubule Architecture Specified by a beta -Tubulin Isoform

Elizabeth C. Raff, * James D. Fackenthal, dagger Jeffrey A. Hutchens, Henry D. Hoyle, F. Rudolf Turner

In Drosophila melanogaster, a testis-specific beta -tubulin (beta 2) is required for spermatogenesis. A sequence motif was identified in carboxyl termini of axonemal beta -tubulins in diverse taxa. As a test of whether orthologous beta -tubulins from different species are functionally equivalent, the moth Heliothis virescens beta 2 homolog was expressed in Drosophila testes. When coexpressed with beta 2, the moth isoform imposed the 16-protofilament structure characteristic of that found in the moth on the corresponding subset of Drosophila microtubules, which normally contain only 13-protofilament microtubules. Thus, the architecture of the microtubule cytoskeleton can be directed by a component beta -tubulin.

Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, IN 47405, USA.
*   To whom correspondence should be addressed. E-mail: eraff{at}bio.indiana.edu

dagger    Present address: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.

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