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Science 20 December 1996:
Vol. 274. no. 5295, pp. 2092 - 2094
DOI: 10.1126/science.274.5295.2092
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Reports

Reexpression of RAG-1 and RAG-2 Genes in Activated Mature Mouse B cells

Masaki Hikida, Masaharu Mori, Toshiyuki Takai, Ken-ichi Tomochika, Kiyohiro Hamatani, Hitoshi Ohmori *

Recombination activating genes (RAG-1 and RAG-2), involved in V(D)J rearrangement of immunoglobulin genes, have been thought to be expressed only in immature stages of B-cell development. However, RAG-1 and RAG-2 transcripts were found to be reexpressed in mature mouse B cells after culture with interleukin-4 in association with several different co-stimuli. Reexpression was also detected in draining lymph nodes from immunized mice. RAG-1 and RAG-2 proteins could be detected by immunofluorescence microscopy in the nuclei of B cells cultured in vitro and in the germinal centers of draining lymph nodes. These findings suggest that RAG gene products play a heretofore unsuspected role in mature B cells.

M. Hikida, T. Takai, H. Ohmori, Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700, Japan.
M. Mori, Faculty of Health and Welfare Sciences, Okayama Prefectural University, Soja 719-11, Japan.
K. Tomochika, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700, Japan.
K. Hamatani, Radiation Effects Research Foundation (Hiroshima), Hiroshima 732, Japan.
*   To whom correspondence should be addressed. E-mail: hit2224{at}cc.okayama-u.ac.jp

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Science. ISSN 0036-8075 (print), 1095-9203 (online)