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Science 15 November 1996:
Vol. 274. no. 5290, pp. 1201 - 1204
DOI: 10.1126/science.274.5290.1201
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Reports

Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae

Wolfgang Zachariae, * Tae Ho Shin, *dagger Marta Galova, Brigitte Obermaier, Kim Nasmyth ddagger

Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans. A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.

W. Zachariae, T. H. Shin, M. Galova, K. Nasmyth, Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
B. Obermaier, MediGene GmbH, Lochhamer Str. 11, 82152 Martinsried, Germany.
*   These authors contributed equally to this work.

dagger    Present address: University of Massachusetts, Cancer Center, 373 Plantation Street, Worcester, MA 01605, USA.

ddagger    To whom correspondence should be addressed.

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