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Science 8 November 1996:
Vol. 274. no. 5289, pp. 998 - 1001
DOI: 10.1126/science.274.5289.998
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Reports

Laser Capture Microdissection

Michael R. Emmert-Buck, Robert F. Bonner, Paul D. Smith, Rodrigo F. Chuaqui, Zhengping Zhuang, Seth R. Goldstein, Rhonda A. Weiss, Lance A. Liotta *

Laser capture microdissection (LCM) under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section of complex, heterogeneous tissue. In this technique, a transparent thermoplastic film (ethylene vinyl acetate polymer) is applied to the surface of the tissue section on a standard glass histopathology slide; a carbon dioxide laser pulse then specifically activates the film above the cells of interest. Strong focal adhesion allows selective procurement of the targeted cells. Multiple examples of LCM transfer and tissue analysis, including polymerase chain reaction amplification of DNA and RNA, and enzyme recovery from transferred tissue are demonstrated.

M. R. Emmert-Buck, R. F. Chuaqui, Z. Zhuang, R. A. Weiss, L. A. Liotta, Laboratory of Pathology, National Cancer Institute, Room 2A33, Building 10, 9000 Rockville Pike, Bethesda, MD 20892, USA.
R. F. Bonner, P. D. Smith, S. R. Goldstein, Biomedical Engineering and Instrumentation Program, National Center for Research Resources, National Institutes of Health, Bethesda, MD 20892, USA.
*   To whom correspondence should be addressed.

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Estrogen-Responsive Finger Protein as a New Potential Biomarker for Breast Cancer.
T. Suzuki, T. Urano, T. Tsukui, K. Horie-Inoue, T. Moriya, T. Ishida, M. Muramatsu, Y. Ouchi, H. Sasano, and S. Inoue (2005)
Clin. Cancer Res. 11, 6148-6154
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Genetic Classification of Lung Adenocarcinoma Based on Array-Based Comparative Genomic Hybridization Analysis: Its Association with Clinicopathologic Features.
T. Shibata, S. Uryu, A. Kokubu, F. Hosoda, M. Ohki, T. Sakiyama, Y. Matsuno, R. Tsuchiya, Y. Kanai, T. Kondo, et al. (2005)
Clin. Cancer Res. 11, 6177-6185
   Abstract »    Full Text »    PDF »
Effect of mechanical stretch on HIF-1{alpha} and MMP-2 expression in capillaries isolated from overloaded skeletal muscles: laser capture microdissection study.
M. Milkiewicz and T. L. Haas (2005)
Am J Physiol Heart Circ Physiol 289, H1315-H1320
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Proteomic Analysis of Apoptotic Pathways Reveals Prognostic Factors in Follicular Lymphoma.
C. Gulmann, V. Espina, E. Petricoin III, D. L. Longo, M. Santi, T. Knutsen, M. Raffeld, E. S. Jaffe, L. A. Liotta, and A. L. Feldman (2005)
Clin. Cancer Res. 11, 5847-5855
   Abstract »    Full Text »    PDF »
A guide to issues in microarray analysis: application to endometrial biology.
C. A White and L. A Salamonsen (2005)
Reproduction 130, 1-13
   Abstract »    Full Text »    PDF »
High-Throughput RNA Isolation Technologies. New Tools for High-Resolution Gene Expression Profiling in Plant Systems.
J.-Y. Lee, M. Levesque, and P. N. Benfey (2005)
Plant Physiology 138, 585-590
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Use of Laser-Capture Microdissection for the Identification of Marker Genes for the Ventromedial Hypothalamic Nucleus.
J. P. Segal, N. R. Stallings, C. E. Lee, L. Zhao, N. Socci, A. Viale, T. M. Harris, M. B. Soares, G. Childs, J. K. Elmquist, et al. (2005)
J. Neurosci. 25, 4181-4188
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Use of Reverse Phase Protein Microarrays and Reference Standard Development for Molecular Network Analysis of Metastatic Ovarian Carcinoma.
K. M. Sheehan, V. S. Calvert, E. W. Kay, Y. Lu, D. Fishman, V. Espina, J. Aquino, R. Speer, R. Araujo, G. B. Mills, et al. (2005)
Mol. Cell. Proteomics 4, 346-355
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Expression of Protein Kinases C {beta}I, {beta}II, and VEGF during the Differentiation of Enamel Epithelium in Tooth Development.
M. Aida, T. Irie, T. Aida, and T. Tachikawa (2005)
Journal of Dental Research 84, 234-239
   Abstract »    Full Text »    PDF »
An Overview of Lung Cancer Genomics and Proteomics.
C. A. Granville and P. A. Dennis (2005)
Am. J. Respir. Cell Mol. Biol. 32, 169-176
   Abstract »    Full Text »    PDF »
In Situ Diversification of the Antibody Repertoire in Chronic Lyme Arthritis Synovium.
S. Ghosh, A. C. Steere, B. D. Stollar, and B. T. Huber (2005)
J. Immunol. 174, 2860-2869
   Abstract »    Full Text »    PDF »
Osteoclast-like cells express receptor activity modifying protein 2: application of laser capture microdissection.
M Nakamura, S Morimoto, Q Yang, T Hisamatsu, N Hanai, Y Nakamura, I Mori, and K Kakudo (2005)
J. Mol. Endocrinol. 34, 257-261
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Reliability and Reproducibility of Gene Expression Measurements Using Amplified RNA from Laser-Microdissected Primary Breast Tissue with Oligonucleotide Arrays.
C. King, N. Guo, G. M. Frampton, N. P. Gerry, M. E. Lenburg, and C. L. Rosenberg (2005)
J. Mol. Diagn. 7, 57-64
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Applications of Laser Capture Microdissection in the Study of Neurodegenerative Disease.
D. G. Standaert (2005)
Arch Neurol 62, 203-205
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Epithelial Expression of Profibrotic Mediators in a Model of Allergen-Induced Airway Remodeling.
M. M. Kelly, R. Leigh, P. Bonniaud, R. Ellis, J. Wattie, M. J. Smith, G. Martin, M. Panju, M. D. Inman, and J. Gauldie (2005)
Am. J. Respir. Cell Mol. Biol. 32, 99-107
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Interactions between Prostaglandin E2, Liver Receptor Homologue-1, and Aromatase in Breast Cancer.
J. Zhou, T. Suzuki, A. Kovacic, R. Saito, Y. Miki, T. Ishida, T. Moriya, E. R. Simpson, H. Sasano, and C. D. Clyne (2005)
Cancer Res. 65, 657-663
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Tumoral Drug Metabolism: Overview and Its Implications for Cancer Therapy.
M. Michael and M.M. Doherty (2005)
J. Clin. Oncol. 23, 205-229
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Modeling of Protein Signaling Networks in Clinical Proteomics.
D.H. GEHO, E.F. PETRICOIN, L.A. LIOTTA, and R.P. ARAUJO (2005)
Cold Spring Harb Symp Quant Biol 70, 517-524
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Proteomic analysis for the early detection and rational treatment of cancer--realistic hope?.
E. M. Posadas, F. Simpkins, L. A. Liotta, C. MacDonald, and E. C. Kohn (2005)
Ann. Onc. 16, 16-22
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Intramyocyte Detection of Epstein-Barr Virus Genome by Laser Capture Microdissection in Patients With Inflammatory Cardiomyopathy.
C. Chimenti, A. Russo, M. Pieroni, F. Calabrese, R. Verardo, G. Thiene, M. A. Russo, A. Maseri, and A. Frustaci (2004)
Circulation 110, 3534-3539
   Abstract »    Full Text »    PDF »
Identification and validation of cell surface antigens for antibody targeting in oncology.
P. Carter, L. Smith, and M. Ryan (2004)
Endocr. Relat. Cancer 11, 659-687
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Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens.
M. A. Perlmutter, C. J.M. Best, J. W. Gillespie, Y. Gathright, S. Gonzalez, A. Velasco, W. M. Linehan, M. R. Emmert-Buck, and R. F. Chuaqui (2004)
J. Mol. Diagn. 6, 371-377
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