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Science 8 November 1996:
Vol. 274. no. 5289, pp. 1005 - 1008
DOI: 10.1126/science.274.5289.1005
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Reports

U1-Mediated Exon Definition Interactions Between AT-AC and GT-AG Introns

Qiang Wu and Adrian R. Krainer *

A minor class of metazoan introns has well-conserved splice sites with 5'-AU-AC-3' boundaries, compared to the 5'-GU-AG-3' boundaries and degenerate splice sites of conventional introns. Splicing of the AT-AC intron 2 of a sodium channel (SCN4A) precursor messenger RNA in vitro did not require inhibition of conventional splicing and required adenosine triphosphate, magnesium, and U12 small nuclear RNA (snRNA). When exon 3 was followed by the 5' splice site from the downstream conventional intron, splicing of intron 2 was greatly stimulated. This effect was U1 snRNA-dependent, unlike the basal AT-AC splicing reaction. Therefore, U1-mediated exon definition interactions can coordinate the activities of major and minor spliceosomes.

Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724-2208, USA.
*   To whom correspondence should be addressed. E-mail: krainer{at}cshl.org

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