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Science 1 November 1996:
Vol. 274. no. 5288, pp. 780 - 782
DOI: 10.1126/science.274.5288.780
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Reports

A Role for Phosphoinositide 3-Kinase in Bacterial Invasion

Keith Ireton, Bernard Payrastre, Hugues Chap, Wataru Ogawa, Hiroshi Sakaue, Masato Kasuga, Pascale Cossart *

Listeria monocytogenes is a bacterial pathogen that invades cultured nonphagocytic cells. Inhibitors and a dominant negative mutation were used to demonstrate that efficient entry requires the phosphoinositide (PI) 3-kinase p85alpha -p110. Infection with L. monocytogenes caused rapid increases in cellular amounts of PI(3,4)P2 and PI(3,4,5)P3, indicating that invading bacteria stimulated PI 3-kinase activity. This stimulation required the bacterial protein InlB, host cell tyrosine phosphorylation, and association of p85alpha with one or more tyrosine-phosphorylated proteins. This role for PI 3-kinase in bacterial entry may have parallels in some endocytic events.

K. Ireton and P. Cossart, Unité des Interactions Bactéries-Cellules, Institut Pasteur, 75724 Paris Cedex 15, France.
B. Payrastre and H. Chap, INSERM Unité 326, Hôpital Purpan, 31059 Toulouse, France.
W. Ogawa, H. Sakaue, M. Kasuga, Second Department of Internal Medicine, Kobe University School of Medicine, Chuo-ku, Kobe 650, Japan.
*   To whom correspondence should be addressed.

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