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Science 25 October 1996:
Vol. 274. no. 5287, pp. 624 - 627
DOI: 10.1126/science.274.5287.624
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Reports

Kap104p: A Karyopherin Involved in the Nuclear Transport of Messenger RNA Binding Proteins

John D. Aitchison, Günter Blobel, * Michael P. Rout

A cytosolic yeast karyopherin, Kap104p, was isolated and shown to function in the nuclear import of a specific class of proteins. The protein bound directly to repeat-containing nucleoporins and to a cytosolic pool of two nuclear messenger RNA (mRNA) binding proteins, Nab2p and Nab4p. Depletion of Kap104p resulted in a rapid shift of Nab2p from the nucleus to the cytoplasm without affecting the localization of other nuclear proteins tested. This finding suggests that the major function of Kap104p lies in returning mRNA binding proteins to the nucleus after mRNA export.

Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
*   To whom correspondence should be addressed. E-mail: blobel{at}rockvax.rockefeller.edu

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The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export.
J. L. Watkins, R. Murphy, J. L. T. Emtage, and S. R. Wente (1998)
PNAS 95, 6779-6784
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Identification and Functional Characterization of a Novel Nuclear Localization Signal Present in the Yeast Nab2 Poly(A)+ RNA Binding Protein.
R. Truant, R. A. Fridell, R. E. Benson, H. Bogerd, and B. R. Cullen (1998)
Mol. Cell. Biol. 18, 1449-1458
   Abstract »    Full Text »
HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection.
M. A. Vodicka, D. M. Koepp, P. A. Silver, and M. Emerman (1998)
Genes & Dev. 12, 175-185
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Active nuclear pore complexes in Chironomus: visualization of transporter configurations related to mRNP export.
E Kiseleva, M. Goldberg, T. Allen, and C. Akey (1998)
J. Cell Sci. 111, 223-236
   Abstract »    PDF »
A Distinct and Parallel Pathway for the Nuclear Import of an mRNA-binding Protein.
L. F. Pemberton, J. S. Rosenblum, and G. Blobel (1997)
J. Cell Biol. 139, 1645-1653
   Abstract »    Full Text »    PDF »
A Nuclear Import Pathway for a Protein Involved in tRNA Maturation.
J. S. Rosenblum, L. F. Pemberton, and G. Blobel (1997)
J. Cell Biol. 139, 1655-1661
   Abstract »    Full Text »    PDF »
Evolutionary specialization of the nuclear targeting apparatus.
H. S. Malik, T. H. Eickbush, and D. S. Goldfarb (1997)
PNAS 94, 13738-13742
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Ran-unassisted Nuclear Migration of a 97-kD Component of Nuclear Pore-targeting Complex.
S. Kose, N. Imamoto, T. Tachibana, T. Shimamoto, and Y. Yoneda (1997)
J. Cell Biol. 139, 841-849
   Abstract »    Full Text »    PDF »


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